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Default samplesheet could be included in ./assets, and the --lp_* parameters could be used to modify it.
Requirements:
add parameters to set (and overwrite) the pcr_primers and target_gene fields in the loci_params sheet (probably use --pcr_primers and --target_gene parameters)
support for lists
parse_inputs.R needs to use above parameters to edit the loci_params sheet (imported from default in ./assets) and then parse the replacement parameters
change channel logic so default .csv is used if --loci_params is unset
pipeline schema needs to be updated to allow --loci_params to be unset if --pcr_primers and --target_gene are set
Reason to do this is to eliminate requirement of creating a loci_params.csv in addition in to the samplesheet, for flexibility. But overall, .csv files probably preferred for complex experimental designs of three or more multiplexed loci, as lists of parameters get difficult
Note: single values passed to --lp_* overwrite all rows of the sheet, which is convenient for the parameters that are likely to be the same for all primers
The text was updated successfully, but these errors were encountered:
Default samplesheet could be included in
./assets
, and the--lp_*
parameters could be used to modify it.Requirements:
pcr_primers
andtarget_gene
fields in theloci_params
sheet (probably use--pcr_primers
and--target_gene
parameters)parse_inputs.R
needs to use above parameters to edit theloci_params
sheet (imported from default in./assets
) and then parse the replacement parameters--loci_params
is unset--loci_params
to be unset if--pcr_primers
and--target_gene
are setReason to do this is to eliminate requirement of creating a
loci_params.csv
in addition in to the samplesheet, for flexibility. But overall, .csv files probably preferred for complex experimental designs of three or more multiplexed loci, as lists of parameters get difficultNote: single values passed to
--lp_*
overwrite all rows of the sheet, which is convenient for the parameters that are likely to be the same for all primersThe text was updated successfully, but these errors were encountered: