samtools parameter not available

[lmanchon]

samtools.1.14

failed with:
samtools view: invalid option -- 'N'

Usage: samtools view [options] <in.bam>|<in.sam>|<in.cram> [region ...]
samtools view: failed to open "3:139527479-139527599" for reading: No such file or directory
Use of uninitialized value $sample in join or string at /save/user/Seq2C/seq2cov.pl line 138.
Use of uninitialized value $sample in join or string at /save/user/Seq2C/seq2cov.pl line 145.
samtools view: failed to open "9:21816468-21816588" for reading: No such file or directory
Use of uninitialized value $sample in join or string at /save/user/Seq2C/seq2cov.pl line 138.
samtools view: failed to open "9:21920286-21920406" for reading: No such file or directory