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[siamaera] Blast exited with error: -1 #127

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SC-Duan opened this issue May 3, 2018 · 0 comments
Open

[siamaera] Blast exited with error: -1 #127

SC-Duan opened this issue May 3, 2018 · 0 comments

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@SC-Duan
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SC-Duan commented May 3, 2018

Hi,
I got an error when using proovread, the error like this:
[01:24:59]/program/proovread/proovread/bin/SeqFilte
r-1.06
[01:24:59] --in: E/E.untrimmed.fq
[01:24:59] Detected FASTQ format, phred-offset 33
[01:24:59] --substr-file: E/E.chim.tsv
0 [ ]Can't exec "/bin/sh": Argument list too long at /program/proovread/proovread/bin/siamaera line 520, line 380.
[18-04-30 01:25:36] [siamaera] Blast exited with error: -1
#------------------------------------------------------------------------------#

[Mon Apr 30 01:25:36 2018] Converting to FASTA file
#------------------------------------------------------------------------------#
perl -I/program/proovread/proovread/bin/../lib/ /program/proovread/proovread/bin/SeqFilter --fasta --i
n E/E.trimmed.fq --out E/E.trimmed.fa --phred-offset 33
[01:25:37]/program/proovread/proovread/bin/SeqFilte
r-1.06
[01:25:37] --in: E/E.trimmed.fq
[01:25:37] Detected FASTQ format, phred-offset 33
^M 57.3k [=> ]^M 3.20M [=========================================================>] TTS 00:00:00
#------------------------------------------------------------------------------#
[01:25:37] Input
[01:25:37] Sequences 75 #
[01:25:37] Total 1597471 bp
[01:25:37] Longest 81322 bp
[01:25:37] Shortest 717 bp
[01:25:37] N50 42009 bp
[01:25:37] N90 12987 bp
#------------------------------------------------------------------------------#
[01:25:37] Filtered
[01:25:37] Sequences 75 #
[01:25:37] Total 1597471 bp
[01:25:37] Longest 81322 bp
[01:25:37] Shortest 717 bp
[01:25:37] N50 42009 bp
[01:25:37] N90 12987 bp
#------------------------------------------------------------------------------#

#------------------------------------------------------------------------------#
[Mon Apr 30 01:25:37 2018] Cleaning up
Mon Apr 30 01:26:24 2018] Total time spent 13:58:08
[Mon Apr 30 01:26:24 2018] Done

The genome size is about 2.5Gb. Can you give me some help?
Thanks a lot!

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