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Stitching Issues #137

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Tas-V opened this issue Mar 26, 2024 · 1 comment
Open

Stitching Issues #137

Tas-V opened this issue Mar 26, 2024 · 1 comment

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@Tas-V
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Tas-V commented Mar 26, 2024

I am trying to stitch a brain that had wobbly scanning but am not getting good results. I am facing two problems:

  1. Black tiles
  2. misaligned outer cortex at tile overlap in some tiles

I have included pictures of both issues; the second issue in particular is strange, as it seems that the wobbly stitcher is not moving tiles in x/y from the rigid alignment. It has determined that the misaligned area seems to be the best area. I have tried skipping the rigid stitching step but still face the same issue. Are the cells too dense for proper stitching to be done?

img1: rigid stitching preview
img2: wobbly stitch image (no rigid alignment), misaligned outer cortex and black tile

image
image

@noooo123456
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Hello!
Check the actual overlap in ImageJ in adjusted tiles.
for the black tiles my colleague found a solution: go to wobbly settings and reduce minimum slice range and valid range to 0 and slice pixel size to 100.

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