diff --git a/backend/audit/fixtures/workbooks/should_fail/federal-awards/has-bad-aln-prefixes.xlsx b/backend/audit/fixtures/workbooks/should_fail/federal-awards/has-bad-aln-prefixes.xlsx
new file mode 100644
index 0000000000..19223f11d4
Binary files /dev/null and b/backend/audit/fixtures/workbooks/should_fail/federal-awards/has-bad-aln-prefixes.xlsx differ
diff --git a/backend/audit/fixtures/workbooks/should_pass/aln_read_as_decimal_values/has-aln-prefix-returned-as-decimal-numbers.xlsx b/backend/audit/fixtures/workbooks/should_pass/aln_read_as_decimal_values/has-aln-prefix-returned-as-decimal-numbers.xlsx
new file mode 100644
index 0000000000..bc8c0e6c6f
Binary files /dev/null and b/backend/audit/fixtures/workbooks/should_pass/aln_read_as_decimal_values/has-aln-prefix-returned-as-decimal-numbers.xlsx differ
diff --git a/backend/audit/intakelib/checks/check_aln_prefix_pattern.py b/backend/audit/intakelib/checks/check_aln_prefix_pattern.py
new file mode 100644
index 0000000000..18d63120ed
--- /dev/null
+++ b/backend/audit/intakelib/checks/check_aln_prefix_pattern.py
@@ -0,0 +1,33 @@
+import logging
+import re
+from django.conf import settings
+
+from django.core.exceptions import ValidationError
+from ..intermediate_representation import (
+    get_range_by_name,
+)
+from ..common import get_message, build_cell_error_tuple
+
+logger = logging.getLogger(__name__)
+
+
+# DESCRIPTION
+# The ALN prefix represents a two-digit number ##
+# TESTED BY has_bad_aln_prefix.xlsx
+def aln_agency_prefix(ir):
+    prefixes = get_range_by_name(ir, "federal_agency_prefix")
+    errors = []
+    for index, prefix in enumerate(prefixes["values"]):
+        if not re.match(settings.REGEX_ALN_PREFIX, str(prefix)):
+            errors.append(
+                build_cell_error_tuple(
+                    ir,
+                    prefixes,
+                    index,
+                    get_message("check_aln_prefix_invalid"),
+                )
+            )
+
+    if len(errors) > 0:
+        logger.info("Raising a validation error.")
+        raise ValidationError(errors)
diff --git a/backend/audit/intakelib/checks/check_finding_award_references_pattern.py b/backend/audit/intakelib/checks/check_finding_award_references_pattern.py
index e265211253..838d54e7c4 100644
--- a/backend/audit/intakelib/checks/check_finding_award_references_pattern.py
+++ b/backend/audit/intakelib/checks/check_finding_award_references_pattern.py
@@ -11,7 +11,7 @@
 
 logger = logging.getLogger(__name__)
 
-# A version of these regexes also exists in Base.libsonnet
+# A version of this regex also exists in Base.libsonnet
 AWARD_REFERENCES_REGEX = r"^AWARD-(?!0000)[0-9]{4}$"
 
 
diff --git a/backend/audit/intakelib/checks/runners.py b/backend/audit/intakelib/checks/runners.py
index 4ef792a63a..f3a5e2f487 100644
--- a/backend/audit/intakelib/checks/runners.py
+++ b/backend/audit/intakelib/checks/runners.py
@@ -50,6 +50,7 @@
 from .check_no_repeat_findings import no_repeat_findings
 from .check_findings_grid_validation import findings_grid_validation
 from .check_finding_prior_references_pattern import prior_references_pattern
+from .check_aln_prefix_pattern import aln_agency_prefix
 
 logger = logging.getLogger(__name__)
 
@@ -75,6 +76,7 @@
     no_major_program_no_type,
     all_unique_award_numbers,
     sequential_award_numbers,
+    aln_agency_prefix,
     aln_three_digit_extension,
     additional_award_identification,
     federal_program_total_is_correct,
diff --git a/backend/audit/intakelib/common/error_messages.py b/backend/audit/intakelib/common/error_messages.py
index f2bd641dd7..e5d0e2b33c 100644
--- a/backend/audit/intakelib/common/error_messages.py
+++ b/backend/audit/intakelib/common/error_messages.py
@@ -66,6 +66,7 @@
     "check_aln_three_digit_extension_invalid": "The three digit extension should follow one of these formats: ###, RD#, or U##, where # represents a number",
     "check_prior_references_invalid": "Prior references must be <b>N/A</b> or a comma-separated list of values in the format <b>20##-###</b>, for example, <b>2019-001, 2019-002</b>",
     "check_award_references_invalid": "Award references must be of the form <b>AWARD-####</b>, for example, <b>AWARD-0001</b>",
+    "check_aln_prefix_invalid": "The federal agency prefix should be a two-digit value, for example, <b>10</b>",
     "check_additional_award_identification_present": "Missing additional award identification",
     "check_federal_program_total": "Federal program total is {}, but should be {}",
     "check_cluster_total": "This cluster total is {}, but should be {}",
diff --git a/backend/audit/intakelib/transforms/runners.py b/backend/audit/intakelib/transforms/runners.py
index e68d333a96..bff49412bb 100644
--- a/backend/audit/intakelib/transforms/runners.py
+++ b/backend/audit/intakelib/transforms/runners.py
@@ -42,6 +42,7 @@
 from .xform_uniform_cluster_names import regenerate_uniform_cluster_names
 from .xform_reformat_prior_references import reformat_prior_references
 from .xform_reformat_award_references import reformat_award_reference
+from .xform_reformat_agency_prefix import reformat_federal_agency_prefix
 
 logger = logging.getLogger(__name__)
 
@@ -105,6 +106,7 @@ def run_all_secondary_auditors_transforms(ir):
     convert_number_of_findings_to_integers,
     convert_loan_balance_to_integers_or_na,
     regenerate_uniform_cluster_names,
+    reformat_federal_agency_prefix,
     generate_cfda_keys,
 ]
 
diff --git a/backend/audit/intakelib/transforms/xform_reformat_agency_prefix.py b/backend/audit/intakelib/transforms/xform_reformat_agency_prefix.py
new file mode 100644
index 0000000000..b0fc349285
--- /dev/null
+++ b/backend/audit/intakelib/transforms/xform_reformat_agency_prefix.py
@@ -0,0 +1,15 @@
+import logging
+from audit.intakelib.intermediate_representation import (
+    get_range_by_name,
+    replace_range_by_name,
+)
+
+logger = logging.getLogger(__name__)
+
+
+# Tested by has-aln-prefix-entered-as-numbers.xlsx
+def reformat_federal_agency_prefix(ir):
+    references = get_range_by_name(ir, "federal_agency_prefix")
+    new_values = list(map(lambda v: v.split(".")[0] if v else v, references["values"]))
+    new_ir = replace_range_by_name(ir, "federal_agency_prefix", new_values)
+    return new_ir
diff --git a/backend/audit/intakelib/transforms/xform_reformat_award_references.py b/backend/audit/intakelib/transforms/xform_reformat_award_references.py
index b9b7b4a683..9275fab191 100644
--- a/backend/audit/intakelib/transforms/xform_reformat_award_references.py
+++ b/backend/audit/intakelib/transforms/xform_reformat_award_references.py
@@ -7,7 +7,7 @@
 logger = logging.getLogger(__name__)
 
 
-# Tested by has_lowercase_award_reference.py
+# Tested by has_lowercase_award_reference.xlsx
 def reformat_award_reference(ir):
     references = get_range_by_name(ir, "award_reference")
     new_values = list(map(lambda v: v.upper() if v else v, references["values"]))
diff --git a/backend/schemas/source/base/Base.libsonnet b/backend/schemas/source/base/Base.libsonnet
index e713ac060e..eb7709ba34 100644
--- a/backend/schemas/source/base/Base.libsonnet
+++ b/backend/schemas/source/base/Base.libsonnet
@@ -67,8 +67,8 @@ local Meta = {
   },
 };
 
-local REGEX_ALN_PREFIX = '^([0-9]{2})$';
 # A python version of these regexes also exists in settings.py
+local REGEX_ALN_PREFIX = '^([0-9]{2})$';
 local REGEX_RD_EXTENSION = 'RD[0-9]?';
 local REGEX_THREE_DIGIT_EXTENSION = '[0-9]{3}[A-Za-z]{0,1}';
 local REGEX_U_EXTENSION = 'U[0-9]{2}';
diff --git a/backend/static/js/globals.js b/backend/static/js/globals.js
index 11eff355f0..401701c42d 100644
--- a/backend/static/js/globals.js
+++ b/backend/static/js/globals.js
@@ -1,4 +1,4 @@
-export const UPLOAD_TIMEOUT = 30000; // 30s
+export const UPLOAD_TIMEOUT = 60000; // 60s
 
 export const UPLOAD_URLS = {
   'federal-awards': 'federal-awards-expended',