README.md

pyroptosis_signature_data_analysis

Analysis Repository for the Interstellar project. For image analysis and image-based profiling for this project, please see pyroptosis_signature_image_profiling

Workflow:

Cells were incubated for 1h in the inhibitors, and then incubated for 6h in each inducer. For more information on plate layout and experimental design please see pyroptosis_signature_image_profiling.

Treatments of Interest (All in this table are with DMSO 0.025% inhibitor)

Inducer	Number of Doses	Dose Concentration(s)	Function
DMSO	1	0.025%	Vehicle Control
Thapsigargin	2	1uM 10uM	ER Stress Induced Apoptosis
H202	2	100nM 100uM	Cell Death
Flagellin	2	0.1ug/mL 1.0ug/mL	Induce Pyroptosis
LPS	3	0.01ug/mL 1.0ug/mL 100ug/mL	Induce Pyroptosis
LPS + Nigericin	1 * 3 = (3)	1ug/mL + 1uM 1ug/mL + 3uM 1ug/mL + 10uM	Induce Pyroptosis

Other Treatments and combinations

Inducers	# of Doses	Dose Concentration(s)	Inhibitors	# of Doses	Dose Concentration(s)	Function
Media	1	0	Media	1	0	Control
DMSO	1	0.0250%	DMSO	2	0.025% 1.0%	Vehicle Control
DMSO	1	0.0250%	Z-VAD-FMK	2	30uM 100 uM	Inhibit Inflamasome
Thapsigargin	2	1uM 10uM	DMSO	1	0.025%	ER Stress Induced Apoptosis
H202	2	100nM 100uM	DMSO	1	0.025%	Cell Death
H202	2	100nM 100uM	Z-VAD-FMK	1	100uM	Inhibit Inflamasome
H202	2	100nM 100uM	Disulfiram	1	1uM	Cell Death
Flagellin	2	0.1ug/mL 1ug/mL	DMSO	1	0.025%	Induce Pyroptosis
Flagellin	2	0.1ug/mL 1ug/mL	Disulfiram	1	1uM	Induce Pyroptosis Inhibit Inflamasome
LPS	5	0.01ug/mL 0.1ug/mL 1.0ug/mL 10.0ug/mL 100.0 ug/mL	DMSO	1	0.025%	Induce Pyroptosis
LPS	1	10.0ug/mL	Z-VAD-FMK	1	100uM	Induce Pyroptosis / Inhibit Inflamasome
LPS	1	10.0ug/mL	Disulfiram	3	0.1uM 1uM 2.5uM	Induce Pyroptosis / Inhibit Inflamasome
LPS + Nigercin	2 * 3 = (6)	1ug/mL+1ug/mL 1ug/mL+3ug/mL 1ug/mL+10ug/mL 100ug/mL+1ug/mL 100ug/mL+3ug/mL 100ug/mL+10ug/mL	DMSO	1	0.025%	Induce Pyroptosis
LPS + Nigercin	1 * 1 = (1)	1ug/mL+10ug/mL	Z-VAD-FMK	1	100uM	Induce Pyroptosis Inhibit Inflamasome
LPS + Nigercin	1 * 1 = (1)	1ug/mL+10ug/mL	Disulfiram	1	1uM	Induce Pyroptosis Inhibit Inflamasome
Disulfiram	3	0.1uM 1uM 2.5uM	DMSO	1	0.025%	Pyroptosis Inhibitor