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panic: the protein FDR filter didn't reach the desired threshold, try a higher threshold using the --prot parameter #511

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FloorWur opened this issue Nov 20, 2024 · 0 comments

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@FloorWur
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Since I use fragpipe V22 it gives an error message: Process 'PhilosopherFilter' finished, exit code: 2 Process returned non-zero exit code, stopping.
With Fragpipe V19 I never had that error (with the same data set). I have tried different things like higher threshold & reinstall. I have tried running with a smaller dataset and then I don't get an error... But with V19 I could run with large data sets... Can you help me with this error?

Information:
DIA measurements
workflow: DIA-Speclib_quant
MS fragger: Closed Search default config
Quantification with DIA NN

log:
log_2024-11-18_08-40-32.txt

@fcyu fcyu transferred this issue from Nesvilab/FragPipe Nov 20, 2024
@fcyu fcyu changed the title error running Fragpipe V22: 'philosopherFilter'finished, exit code: 2 panic: the protein FDR filter didn't reach the desired threshold, try a higher threshold using the --prot parameter Nov 20, 2024
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