Creating h5ad file for SEVtras.ESAI_calculator

[fusca9]

Hello respected author. I apologize in advance for the inconvenience. I have the following question:
I have to use SEVtras.ESAI_calculator, one of the input files comes from SEVtras recognizer and the other one should be an h5ad file that has barcodes as indexes and a batch column. I know that i have to use the filtered_feature_bc_matrix.H5 file, i have to convert it in an h5ad file and also add the batch column. But i have 18 samples and so 18 filtered_feature_bc_matrix.H5 files and i think that i have to concatenate the converted files into one single file h5ad. how can i do this? am I on the right track? and how can i eventually manage duplicates indexes during the concatenation? because there are duplicates indexes between the samples.
i want to thank you in advance for your availability.

[RuiqiaoHe]

Sorry for the delay because I was at the Chinese New Year vocation.
Yes, you are on the right track. You can concatenate the 18 samples with the following code:
import anndata as ad
adata_com = ad.concat([a, b, c, d, e ...., f, i, j, k], label="batch")
adata_com.obs_names_make_unique()
Here, "a, b, c ... i, j, k" are the names of the 18 samples that you have read.