Notes from Meeting 11/13/19

[gwaybio]

• The confluence of cells appear to be a big issue
  • The current molecular pipeline is to seed at uniform density
  • If we generate additional plates, seeded at different densities, we can test which densities are best for specific clones
  • I will also add cell count to the subcluster morpheus heatmap (see Add Cell Count to SubCluster Morpheus Heatmap #33)
• Next steps:
  • @bethac07 and team will run image analysis pipeline on new data
  • @mekelley and Adi (we need to get Adi on github! 😄 ) will generate new data with different seeding concentrations (see above)
  • I will send out list of top 10-15 features (based on certain metrics) to send to biologists for interpretation (@bethac07, @mekelley, Adi, and @AnneCarpenter) (see Compile list of top 10 or so morphology features indicative of resistance mechanism #34)