diff --git a/cocos/scene-graph/node.ts b/cocos/scene-graph/node.ts
index 294c8d242fa..ccac05857fd 100644
--- a/cocos/scene-graph/node.ts
+++ b/cocos/scene-graph/node.ts
@@ -1525,7 +1525,7 @@ export class Node extends CCObject implements ISchedulable, CustomSerializable {
     @serializable
     protected _euler = new Vec3();
 
-    protected _transformFlags = TransformBit.NONE; // does the world transform need to update?
+    protected _transformFlags = TransformBit.TRS; // does the world transform need to update?
     protected _eulerDirty = false;
 
     protected _flagChangeVersion = 0;
diff --git a/native/cocos/core/scene-graph/Node.h b/native/cocos/core/scene-graph/Node.h
index d08e12fde20..38baeed0324 100644
--- a/native/cocos/core/scene-graph/Node.h
+++ b/native/cocos/core/scene-graph/Node.h
@@ -657,7 +657,7 @@ class Node : public CCObject {
     // NOTE: TypeArray created in node.jsb.ts _ctor should have the same memory layout
     uint32_t _eventMask{0};                                             // Uint32: 0
     uint32_t _layer{static_cast<uint32_t>(Layers::LayerList::DEFAULT)}; // Uint32: 1
-    uint32_t _transformFlags{0};                                        // Uint32: 2
+    uint32_t _transformFlags{static_cast<uint32_t>(TransformBit::TRS)}; // Uint32: 2
     index_t _siblingIndex{0};                                           // Int32: 0
     uint8_t _activeInHierarchy{0};                                      // Uint8: 0
     uint8_t _active{1};                                                 // Uint8: 1
diff --git a/tests/scene-graph/scene.test.ts b/tests/scene-graph/scene.test.ts
new file mode 100644
index 00000000000..76e1cae9e20
--- /dev/null
+++ b/tests/scene-graph/scene.test.ts
@@ -0,0 +1,26 @@
+import { deserialize } from "../../cocos/serialization";
+import '../utils/matchers/value-type-asymmetric-matchers';
+import { v3 } from "../../cocos/core";
+import { Scene } from "../../cocos/scene-graph";
+
+test(`Bugfix cocos/cocos-engine#16352: deserialized scene should have node transforms invalidated`, () => {
+    const archive = [{
+        __type__: 'cc.Scene',
+        _children: [{
+            __type__: 'cc.Node',
+            _name: 'Node',
+            _lscale: {
+                __type__: 'cc.Vec3',
+                x: 2,
+                y: 1,
+                z: 1,
+            },
+        }],
+    }];
+
+    const scene = deserialize(archive) as Scene;
+
+    const node = scene.getChildByPath('Node')!;
+    expect(node).not.toBeNull();
+    expect(node.worldScale).toBeCloseToVec3(v3(2, 1, 1));
+});
\ No newline at end of file