diff --git a/cocos/spine/lib/spine-core.d.ts b/cocos/spine/lib/spine-core.d.ts
index 8211706e3be..721a3908397 100644
--- a/cocos/spine/lib/spine-core.d.ts
+++ b/cocos/spine/lib/spine-core.d.ts
@@ -1191,6 +1191,7 @@ declare namespace spine {
         isDelete: boolean;
         enable: boolean;
         setTrackEntryListener: any;
+        destroy();
         initSkeleton(data: SkeletonData);
         getAnimationState();
         setAnimation(trackIndex: number, name: string, loop: boolean): spine.TrackEntry | null;
diff --git a/cocos/spine/skeleton-cache.ts b/cocos/spine/skeleton-cache.ts
index 4fd1b219b55..869137a5eaa 100644
--- a/cocos/spine/skeleton-cache.ts
+++ b/cocos/spine/skeleton-cache.ts
@@ -76,7 +76,7 @@ export interface AnimationFrame {
 }
 
 export class AnimationCache {
-    protected _instance: spine.SkeletonInstance = null!;
+    protected _instance: spine.SkeletonInstance | null = null;
     protected _state: spine.AnimationState = null!;
     protected _skeletonData: spine.SkeletonData = null!;
     protected _skeleton: spine.Skeleton = null!;
@@ -117,7 +117,7 @@ export class AnimationCache {
 
     public setSkin (skinName: string): void {
         if (this._skeleton) this._skeleton.setSkinByName(skinName);
-        this._instance.setSkin(skinName);
+        this._instance!.setSkin(skinName);
     }
 
     public setAnimation (animationName: string): void {
@@ -134,7 +134,7 @@ export class AnimationCache {
         }
         this._maxFrameIdex = Math.floor((animation as any).duration / FrameTime);
         if (this._maxFrameIdex <= 0) this._maxFrameIdex = 1;
-        this._instance.setAnimation(0, animationName, false);
+        this._instance!.setAnimation(0, animationName, false);
     }
 
     public updateToFrame (frameIdx: number): void {
@@ -145,8 +145,8 @@ export class AnimationCache {
             // Solid update frame rate 1/60.
             this._frameIdx++;
             this.totalTime += FrameTime;
-            this._instance.updateAnimation(FrameTime);
-            const model = this._instance.updateRenderData();
+            this._instance!.updateAnimation(FrameTime);
+            const model = this._instance!.updateRenderData();
             this.updateRenderData(this._frameIdx, model);
             if (this._frameIdx >= this._maxFrameIdex) {
                 this.isCompleted = true;
@@ -234,7 +234,7 @@ export class AnimationCache {
             }
         }
         const listener = skeletonInfo?.listener;
-        this._instance.setAnimation(0, this._animationName!, false);
+        this._instance!.setAnimation(0, this._animationName!, false);
         this.bind(listener!);
 
         // record cur animation cache
@@ -302,7 +302,10 @@ export class AnimationCache {
     }
 
     public destroy (): void {
-        spine.SkeletonSystem.destroySpineInstance(this._instance);
+        if (this._instance) {
+            this._instance.destroy();
+            this._instance = null;
+        }
     }
 }
 
diff --git a/cocos/spine/skeleton-system.ts b/cocos/spine/skeleton-system.ts
index 3d4a1e40606..109f6b7b3cd 100644
--- a/cocos/spine/skeleton-system.ts
+++ b/cocos/spine/skeleton-system.ts
@@ -78,7 +78,6 @@ export class SkeletonSystem extends System {
         if (!this._skeletons) {
             return;
         }
-        if (!EDITOR_NOT_IN_PREVIEW && !JSB) spine.SkeletonSystem.updateAnimation(dt);
         this._skeletons.forEach((skeleton) => {
             skeleton.updateAnimation(dt);
         });
diff --git a/cocos/spine/skeleton.ts b/cocos/spine/skeleton.ts
index 5f47a2cdee9..52ca9bd1f01 100644
--- a/cocos/spine/skeleton.ts
+++ b/cocos/spine/skeleton.ts
@@ -237,7 +237,7 @@ export class Skeleton extends UIRenderer {
 
     protected _runtimeData: spine.SkeletonData | null = null;
     public _skeleton: spine.Skeleton = null!;
-    protected _instance: spine.SkeletonInstance = null!;
+    protected _instance: spine.SkeletonInstance | null = null;
     protected _state: spine.AnimationState = null!;
     protected _textures: Texture2D[] = [];
     private _skeletonInfo: SkeletonCacheItemInfo | null = null;
@@ -491,7 +491,7 @@ export class Skeleton extends UIRenderer {
     set premultipliedAlpha (v: boolean) {
         if (v !== this._premultipliedAlpha) {
             this._premultipliedAlpha = v;
-            this._instance.setPremultipliedAlpha(v);
+            this._instance!.setPremultipliedAlpha(v);
             this.markForUpdateRenderData();
         }
     }
@@ -710,8 +710,9 @@ export class Skeleton extends UIRenderer {
         //if (this._cacheMode == AnimationCacheMode.PRIVATE_CACHE) this._animCache?.destroy();
         this._animCache = null;
         SkeletonSystem.getInstance().remove(this);
-        if (!JSB) {
-            spine.SkeletonSystem.destroySpineInstance(this._instance);
+        if (!JSB && this._instance) {
+            this._instance.destroy();
+            this._instance = null;
         }
         this._destroySkeletonInfo(this._skeletonCache);
         this._skeletonCache = null;
@@ -812,9 +813,9 @@ export class Skeleton extends UIRenderer {
                 this._skeleton = this._skeletonInfo.skeleton!;
             }
         } else {
-            this._skeleton = this._instance.initSkeleton(skeletonData);
-            this._state = this._instance.getAnimationState();
-            this._instance.setPremultipliedAlpha(this._premultipliedAlpha);
+            this._skeleton = this._instance!.initSkeleton(skeletonData);
+            this._state = this._instance!.getAnimationState();
+            this._instance!.setPremultipliedAlpha(this._premultipliedAlpha);
         }
         // Recreate render data and mark dirty
         this._flushAssembler();
@@ -929,7 +930,7 @@ export class Skeleton extends UIRenderer {
             }
         } else {
             this._animationName = name;
-            trackEntry = this._instance.setAnimation(trackIndex, name, loop);
+            trackEntry = this._instance!.setAnimation(trackIndex, name, loop);
         }
         this.markForUpdateRenderData();
         return trackEntry;
@@ -1007,7 +1008,7 @@ export class Skeleton extends UIRenderer {
      */
     public setSkin (name: string): void {
         if (this._skeleton) this._skeleton.setSkinByName(name);
-        this._instance.setSkin(name);
+        this._instance!.setSkin(name);
         if (this.isAnimationCached()) {
             if (this._animCache) {
                 this._animCache.setSkin(name);
@@ -1050,6 +1051,8 @@ export class Skeleton extends UIRenderer {
                 return;
             }
             this._updateCache(dt);
+        } else {
+            this._instance!.updateAnimation(dt);
         }
     }
 
@@ -1112,7 +1115,7 @@ export class Skeleton extends UIRenderer {
             const model = this._curFrame.model;
             return model;
         } else {
-            const model = this._instance.updateRenderData();
+            const model = this._instance!.updateRenderData();
             return model;
         }
     }
@@ -1444,7 +1447,7 @@ export class Skeleton extends UIRenderer {
             return;
         }
         if (this._state) {
-            this._instance.setMix(fromAnimation, toAnimation, duration);
+            this._instance!.setMix(fromAnimation, toAnimation, duration);
             //this._state.data.setMix(fromAnimation, toAnimation, duration);
         }
     }
@@ -1577,7 +1580,7 @@ export class Skeleton extends UIRenderer {
         this.destroyRenderData();
         if (!JSB) {
             if (!this.isAnimationCached()) {
-                this._instance.setUseTint(this._useTint);
+                this._instance!.setUseTint(this._useTint);
             }
         }
         if (this._assembler && this._skeleton) {
@@ -1607,7 +1610,7 @@ export class Skeleton extends UIRenderer {
             if (this.isAnimationCached()) {
                 warn('Debug bones or slots is invalid in cached mode');
             } else if (!JSB) {
-                this._instance.setDebugMode(true);
+                this._instance!.setDebugMode(true);
             }
         } else if (this._debugRenderer) {
             this._debugRenderer.node.destroy();
@@ -1655,7 +1658,7 @@ export class Skeleton extends UIRenderer {
         const r = this._color.r / 255.0;
         const g = this._color.g / 255.0;
         const b = this._color.b / 255.0;
-        this._instance.setColor(r, g, b, a);
+        this._instance!.setColor(r, g, b, a);
     }
 
     /**
@@ -1664,6 +1667,9 @@ export class Skeleton extends UIRenderer {
      * @param effectDelegate @en Vertex effect delegate. @zh 顶点特效代理。
      */
     public setVertexEffectDelegate (effectDelegate: VertexEffectDelegate | null | undefined): void {
+        if (!this._instance) {
+            return;
+        }
         if (!effectDelegate) {
             this._instance.clearEffect();
             return;
@@ -1692,7 +1698,7 @@ export class Skeleton extends UIRenderer {
     public setStartListener (listener: TrackListener): void {
         this._ensureListener();
         const listenerID = TrackEntryListeners.addListener(listener);
-        this._instance.setListener(listenerID, spine.EventType.start);
+        this._instance!.setListener(listenerID, spine.EventType.start);
         this._listener!.start = listener;
     }
 
@@ -1704,7 +1710,7 @@ export class Skeleton extends UIRenderer {
     public setInterruptListener (listener: TrackListener): void {
         this._ensureListener();
         const listenerID = TrackEntryListeners.addListener(listener);
-        this._instance.setListener(listenerID, spine.EventType.interrupt);
+        this._instance!.setListener(listenerID, spine.EventType.interrupt);
         this._listener!.interrupt = listener;
     }
 
@@ -1716,7 +1722,7 @@ export class Skeleton extends UIRenderer {
     public setEndListener (listener: TrackListener): void {
         this._ensureListener();
         const listenerID = TrackEntryListeners.addListener(listener);
-        this._instance.setListener(listenerID, spine.EventType.end);
+        this._instance!.setListener(listenerID, spine.EventType.end);
         this._listener!.end = listener;
     }
 
@@ -1728,7 +1734,7 @@ export class Skeleton extends UIRenderer {
     public setDisposeListener (listener: TrackListener): void {
         this._ensureListener();
         const listenerID = TrackEntryListeners.addListener(listener);
-        this._instance.setListener(listenerID, spine.EventType.dispose);
+        this._instance!.setListener(listenerID, spine.EventType.dispose);
         this._listener!.dispose = listener;
     }
 
@@ -1740,7 +1746,7 @@ export class Skeleton extends UIRenderer {
     public setCompleteListener (listener: TrackListener): void {
         this._ensureListener();
         const listenerID = TrackEntryListeners.addListener(listener);
-        this._instance.setListener(listenerID, spine.EventType.complete);
+        this._instance!.setListener(listenerID, spine.EventType.complete);
         this._listener!.complete = listener;
     }
 
@@ -1752,7 +1758,7 @@ export class Skeleton extends UIRenderer {
     public setEventListener (listener: TrackListener2): void {
         this._ensureListener();
         const listenerID = TrackEntryListeners.addListener(listener);
-        this._instance.setListener(listenerID, spine.EventType.event);
+        this._instance!.setListener(listenerID, spine.EventType.event);
         this._listener!.event = listener;
     }
 
@@ -1763,7 +1769,7 @@ export class Skeleton extends UIRenderer {
      * @param listener @en Listener for registering callback functions. @zh 监听器对象，可注册回调方法。
      */
     public setTrackStartListener (entry: spine.TrackEntry, listener: TrackListener): void {
-        TrackEntryListeners.getListeners(entry, this._instance).start = listener;
+        TrackEntryListeners.getListeners(entry, this._instance!).start = listener;
     }
 
     /**
@@ -1773,7 +1779,7 @@ export class Skeleton extends UIRenderer {
      * @param listener @en Listener for registering callback functions. @zh 监听器对象，可注册回调方法。
      */
     public setTrackInterruptListener (entry: spine.TrackEntry, listener: TrackListener): void {
-        TrackEntryListeners.getListeners(entry, this._instance).interrupt = listener;
+        TrackEntryListeners.getListeners(entry, this._instance!).interrupt = listener;
     }
 
     /**
@@ -1783,7 +1789,7 @@ export class Skeleton extends UIRenderer {
      * @param listener @en Listener for registering callback functions. @zh 监听器对象，可注册回调方法。
      */
     public setTrackEndListener (entry: spine.TrackEntry, listener: TrackListener): void {
-        TrackEntryListeners.getListeners(entry, this._instance).end = listener;
+        TrackEntryListeners.getListeners(entry, this._instance!).end = listener;
     }
 
     /**
@@ -1793,7 +1799,7 @@ export class Skeleton extends UIRenderer {
      * @param listener @en Listener for registering callback functions. @zh 监听器对象，可注册回调方法。
      */
     public setTrackDisposeListener (entry: spine.TrackEntry, listener: TrackListener): void {
-        TrackEntryListeners.getListeners(entry, this._instance).dispose = listener;
+        TrackEntryListeners.getListeners(entry, this._instance!).dispose = listener;
     }
 
     /**
@@ -1810,7 +1816,7 @@ export class Skeleton extends UIRenderer {
             // this._instance.setListener(listenerID, spine.EventType.event);
             // this._listener!.event = listener;
         };
-        TrackEntryListeners.getListeners(entry, this._instance).complete = onComplete;
+        TrackEntryListeners.getListeners(entry, this._instance!).complete = onComplete;
     }
 
     /**
@@ -1820,14 +1826,14 @@ export class Skeleton extends UIRenderer {
      * @param listener @en Listener for registering callback functions. @zh 监听器对象，可注册回调方法。
      */
     public setTrackEventListener (entry: spine.TrackEntry, listener: TrackListener|TrackListener2): void {
-        TrackEntryListeners.getListeners(entry, this._instance).event = listener;
+        TrackEntryListeners.getListeners(entry, this._instance!).event = listener;
     }
 
     /**
      * @engineInternal
     */
     public getDebugShapes (): any {
-        return this._instance.getDebugShapes();
+        return this._instance!.getDebugShapes();
     }
 
     /**
@@ -1852,7 +1858,7 @@ export class Skeleton extends UIRenderer {
         const width = tex2d.width;
         const height = tex2d.height;
         const createNewAttachment = createNew || false;
-        this._instance.resizeSlotRegion(slotName, width, height, createNewAttachment);
+        this._instance!.resizeSlotRegion(slotName, width, height, createNewAttachment);
         if (!this._slotTextures) this._slotTextures = new Map<number, Texture2D>();
         let textureID = 0;
         this._slotTextures.forEach((value, key) => {
@@ -1862,7 +1868,7 @@ export class Skeleton extends UIRenderer {
             textureID = ++_slotTextureID;
             this._slotTextures.set(textureID, tex2d);
         }
-        this._instance.setSlotTexture(slotName, textureID);
+        this._instance!.setSlotTexture(slotName, textureID);
     }
 
     private _destroySkeletonInfo (skeletonCache: SkeletonCache | null): void {
diff --git a/native/cocos/editor-support/spine-wasm/spine-skeleton-instance.cpp b/native/cocos/editor-support/spine-wasm/spine-skeleton-instance.cpp
index 08192bf0aea..8b171a4c750 100644
--- a/native/cocos/editor-support/spine-wasm/spine-skeleton-instance.cpp
+++ b/native/cocos/editor-support/spine-wasm/spine-skeleton-instance.cpp
@@ -5,7 +5,6 @@
 #include "spine-mesh-data.h"
 #include "spine-wasm.h"
 #include "util-function.h"
-#include "spine-skeleton-system.h"
 
 SlotMesh globalMesh(nullptr, nullptr, 0, 0);
 
@@ -34,7 +33,6 @@ static void trackEntryCallback(AnimationState *state, EventType type, TrackEntry
 
 SpineSkeletonInstance::SpineSkeletonInstance() {
     _model = new SpineModel();
-    SpineSkeletonSystem::addSpineInstance(this);
 }
 
 SpineSkeletonInstance::~SpineSkeletonInstance() {
@@ -46,6 +44,10 @@ SpineSkeletonInstance::~SpineSkeletonInstance() {
     if (_model) delete _model;
 }
 
+void SpineSkeletonInstance::destroy() {
+    delete this;
+}
+
 Skeleton *SpineSkeletonInstance::initSkeleton(SkeletonData *data) {
     if (_clipper) delete _clipper;
     if (_animState) delete _animState;
diff --git a/native/cocos/editor-support/spine-wasm/spine-skeleton-instance.h b/native/cocos/editor-support/spine-wasm/spine-skeleton-instance.h
index cb8190cbf1c..c1b53149ebf 100644
--- a/native/cocos/editor-support/spine-wasm/spine-skeleton-instance.h
+++ b/native/cocos/editor-support/spine-wasm/spine-skeleton-instance.h
@@ -58,6 +58,7 @@ class SpineSkeletonInstance {
     std::vector<SpineDebugShape> &getDebugShapes();
     void resizeSlotRegion(const std::string &slotName, uint32_t width, uint32_t height, bool createNew = false);
     void setSlotTexture(const std::string &slotName, uint32_t index);
+    void destroy();
     bool isCache{false};
     bool enable{true};
     float dtRate{1.0F};
diff --git a/native/cocos/editor-support/spine-wasm/spine-skeleton-system.cpp b/native/cocos/editor-support/spine-wasm/spine-skeleton-system.cpp
deleted file mode 100644
index 5c2ead7f4a1..00000000000
--- a/native/cocos/editor-support/spine-wasm/spine-skeleton-system.cpp
+++ /dev/null
@@ -1,42 +0,0 @@
-#include "spine-skeleton-system.h"
-#include "spine-skeleton-instance.h"
-#include "spine-mesh-data.h"
-
-using namespace spine;
-
-std::vector<SpineSkeletonInstance*> SpineSkeletonSystem::vectorSpines;
-
-void SpineSkeletonSystem::updateAnimation(float deltaTime) {
-    auto count = static_cast<int>(vectorSpines.size());
-    for (int i = count - 1; i >= 0; --i) {
-        SpineSkeletonInstance* spineInstance = vectorSpines[i];
-        if(!spineInstance->enable) continue;
-        if (!spineInstance->isCache) {
-            spineInstance->updateAnimation(deltaTime);
-        }
-    }
-}
-
-int SpineSkeletonSystem::getCount() {
-   auto count = vectorSpines.size(); 
-   return count;
-}
-
-void SpineSkeletonSystem::updateRenderData() {
-    SpineMeshData::reset();
-}
-
-void SpineSkeletonSystem::addSpineInstance(SpineSkeletonInstance* instance) {
-    if(vectorSpines.size() == vectorSpines.capacity()){
-        vectorSpines.reserve(vectorSpines.size() + 20);
-    }
-    vectorSpines.push_back(instance);
-}
-
-void SpineSkeletonSystem::destroySpineInstance(SpineSkeletonInstance* instance) {
-    auto it = std::find(vectorSpines.begin(), vectorSpines.end(), instance);
-    if (it != vectorSpines.end()) {
-        vectorSpines.erase(it);
-        delete instance;
-    }
-}
\ No newline at end of file
diff --git a/native/cocos/editor-support/spine-wasm/spine-skeleton-system.h b/native/cocos/editor-support/spine-wasm/spine-skeleton-system.h
deleted file mode 100644
index 6be9cd9e860..00000000000
--- a/native/cocos/editor-support/spine-wasm/spine-skeleton-system.h
+++ /dev/null
@@ -1,15 +0,0 @@
-#include <vector>
-#include "spine-skeleton-instance.h"
-
-using namespace spine;
-class SpineSkeletonSystem
-{
-public:
-    static void updateAnimation(float deltaTime);
-    static void updateRenderData();
-    static int getCount();
-    static void addSpineInstance(SpineSkeletonInstance* instance);
-    static void destroySpineInstance(SpineSkeletonInstance* instance);
-private:
-    static std::vector<SpineSkeletonInstance*> vectorSpines;
-};
\ No newline at end of file
diff --git a/native/cocos/editor-support/spine-wasm/spine-type-export.cpp b/native/cocos/editor-support/spine-wasm/spine-type-export.cpp
index 83133336b98..4fc0eac3c8d 100644
--- a/native/cocos/editor-support/spine-wasm/spine-type-export.cpp
+++ b/native/cocos/editor-support/spine-wasm/spine-type-export.cpp
@@ -3,7 +3,6 @@
 #include <functional>
 #include <memory>
 #include <vector>
-#include "spine-skeleton-system.h"
 #include "spine-skeleton-instance.h"
 #include "spine-wasm.h"
 #include "Vector2.h"
@@ -1607,13 +1606,8 @@ EMSCRIPTEN_BINDINGS(spine) {
         .function("setDebugMode", &SpineSkeletonInstance::setDebugMode)
         .function("getDebugShapes", &SpineSkeletonInstance::getDebugShapes)
         .function("resizeSlotRegion", &SpineSkeletonInstance::resizeSlotRegion)
+        .function("destroy", &SpineSkeletonInstance::destroy)
         .function("setSlotTexture", &SpineSkeletonInstance::setSlotTexture);
-
-    class_<SpineSkeletonSystem>("SkeletonSystem")
-        .class_function("getCount", &SpineSkeletonSystem::getCount) 
-        .class_function("updateAnimation", &SpineSkeletonSystem::updateAnimation)
-        .class_function("updateRenderData", &SpineSkeletonSystem::updateRenderData)
-        .class_function("destroySpineInstance", &SpineSkeletonSystem::destroySpineInstance, allow_raw_pointers());
 }
 
 EMSCRIPTEN_BINDINGS(cocos_spine) {
diff --git a/native/external-config.json b/native/external-config.json
index 89a1e20ef94..78ca7978643 100644
--- a/native/external-config.json
+++ b/native/external-config.json
@@ -3,6 +3,6 @@
         "type": "github",
         "owner": "cocos-creator",
         "name": "engine-native-external",
-        "checkout": "v3.8.2-22"
+        "checkout": "v3.8.2-23"
     }
 }
\ No newline at end of file