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<li id="navhome"><a href="#home">Home</a></li>
<li id="navbio"><a href="#bio">David Schaffer</a></li>
<li id="navinterests"><a href="#interests">Research Interests</a></li>
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<div class="item"><a class="group2" href="img/1.jpg" title="Human Corticol Glutamatergic Neurons: Generation of cortical excitatory neurons from human pluripotent stem cells by a novel differentiation paradigm. Neurons (green) glutamate(red). Courtesy of Tandis Vazin"><img class="lazyOwl" data-src="img/1.jpg" /></a></div>
<div class="item"><a class="group2" href="img/2.jpg" title="Confocal image of an adult rat hippocampus, the area of the brain where new memories are formed, at 25X magnification. Astrocytes (white) signal to neural stem cells found below the granular cell layer (green) to differentiate into immature neurons (red). Courtesy of Anthony Conway"><img class="lazyOwl" data-src="img/2.jpg" /></a></div>
<div class="item"><a class="group2" href="img/3orig.jpg" title="Cortical neural cultures derived from human pluripotent stem cells. Courtesy of Tandis Vazin"><img class="lazyOwl" data-src="img/3.jpg" /></a></div>
<div class="item"><a class="group2" href="img/4.jpg" title="Confocal image of a single colony of neuronally differentiated human embryonic stem cells at 10X magnification. Immature neurons (green) project axons throughout the colony as well as out into the surrounding area. Dopaminergic neurons (red), which are the neurons lost in Parkinson's disease, are also found throughout the colony. Courtesy of Anthony Conway"><img class="lazyOwl" data-src="img/4.jpg" /></a></div>
<div class="item"><a class="group2" href="img/5.jpg" title="Confocal image of an adult mouse hippocampus, the area of the brain where new memories are formed, at 100X magnification. β-galactosidase is shown in red, which is driven by an inducible Nestin-Cre reporter, and thus indicates current and differentiated neural stem cells. Cell nuclei are shown in blue. Courtesy of Anthony Conway"><img class="lazyOwl" data-src="img/5.jpg" /></a></div>
<div class="item"><a class="group2" href="img/6.jpg" title="Cofocal images taken around the optic nerve head in rat eyes infected with AAV2 driving expression of GFP with a neuronal promoter. Courtesy of Deniz Dalkara"><img class="lazyOwl" data-src="img/6.jpg" /></a></div>
<div class="item"><a class="group2" href="img/7.jpg" title="Confocal image of an adult rat hippocampus, the area of the brain where new memories are formed, at 25X magnification. Astrocytes (green) signal to neural stem cells (white) to differentiate into immature neurons (red). Cell nuclei are shown in blue. Courtesy of Anthony Conway"><img class="lazyOwl" data-src="img/7.jpg" /></a></div>
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<h1>About the Schaffer Lab</h1>
<p class="lead">The Schaffer research group applies molecular and cellular engineering approaches to investigate biomedical problems focused on engineering of stem cell and gene therapeutics. Our group is a part of the department of Chemical and Biological Engineering, the Helen Wills Neuroscience Institute, and the Bioengineering Graduate Group at UC Berkeley. <br />
<a href="#interests">Click here to read more.</a>
</p>
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<h3>Contact Us</h3>
<p>The Schaffer Lab<br />
University of California, Berkeley<br />
278 Stanley Hall<br />
Berkeley, CA 94720-3220<br />
<br />
Lab Manager/Administrative Assistant:<br />
<a href="mailto:[email protected]">Wanichaya Ramey</a><br />
<br />
Lab Phone: (510) 642-4923<br />
Fax: (510) 642-5198<br />
</p>
</div>
<div class="col-xs-4">
<h3>Group News</h3>
<ul>
<li><a href="http://glennfoundation.org/programs/glennawards/
">David Schaffer received Glenn Award for Research in Biological Mechanisms of Aging.</a></li>
<li><a href="http://newscenter.berkeley.edu/2013/06/12/researchers-develop-easy-and-effective-therapy-to-restore-sight/
">David Schaffer, John Flannery, and colleagues have developed an improved gene therapy vector for treating vision impairment.</a></li>
<li>Deniz and Leah's paper has been accepted into Science Translational Medicine: <a href="http://newscenter.berkeley.edu/2013/06/12/researchers-develop-easy-and-effective-therapy-to-restore-sight/">UC Berkeley News Center</a>, <a href="http://scienceblog.com/63866/researchers-develop-easy-and-effective-therapy-to-restore-sight/">Scienceblog</a>, <a href="http://english.peopledaily.com.cn/90777/8282566.html">Peopledaily</a>, <a href="http://www.ccnmag.com/article/easy_and_effective_therapy_to_restore_sight">California Science & Technology</a>, <a href="http://www.myscience.us/wire/researchers_develop_easy_and_effective_therapy_to_restore_sight-2013-berkeley">Myscience</a>, <a href="http://stm.sciencemag.org/content/5/189/189ra76">Science</a>, <a href="http://www.mdlinx.com/family-medicine/news-article.cfm/4673897/?xml">Mdlinx</a>, <a href="http://www.bing.com/search?q=schaffer+retina+gene+therapy&go=&qs=n&form=QBLH&pq=schaffer+retina+gene+therapy&sc=0-11&sp=-1&sk=">The Scientist and Technology Review</a>, and <a href="http://www.sciencenewsline.com/summary/2013061220230034.html">Sciencenewsline</a>.</li>
</ul>
</div>
<div class="col-xs-4">
<h3>Links</h3>
<ul>
<li><a href="http://cheme.berkeley.edu/" target="_blank">Chemical and Biomolecular Engineering, UCB</a></li>
<li><a href="http://bioeng.berkeley.edu/" target="_blank">Bioengineering, UCB</a></li>
<li><a href="http://neuroscience.berkeley.edu/" title="V">The Helen Wills Neuroscience Institute</a></li>
<li><a href="http://stemcellcenter.berkeley.edu/" target="_blank">The Berkeley Stem Cell Center</a></li>
<li><a href="http://stemcellcenter.berkeley.edu/" target="_blank">CIRM Shared Stem Cell Facility</a></li>
<li><a href=" http://qb3.berkeley.edu" target="_blank">California Institute for <br>Quantitative Biosciences (QB3)</a></li>
<li><a href="http://www.berkeley.edu/" target="_blank">UC Berkeley (UCB)</a></li>
<li><a href="http://www.lbl.gov/" target="_blank">Lawrence Berkeley National Laboratory (LBNL)</a></li>
</ul>
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</div> <!-- /container for home -->
<div class="container webpage" id="bio">
<div class="page-header">
<h1>David V. Schaffer</h1>
</div>
<img class="regular" src="img/schafferbio.jpg" alt="" width="18%">
<p>David Schaffer is a Professor of Chemical and Biomolecular Engineering, Bioengineering, and Neuroscience at University of California, Berkeley, where he also serves as the Director of the <a href="http://stemcellcenter.berkeley.edu/" target="_blank">Berkeley Stem Cell Center</a>. He graduated from Stanford University with a B.S. degree in Chemical Engineering in 1993. Afterward, he attended Massachusetts Institute of Technology and earned his Ph.D. also in Chemical Engineering in 1998 with Professor Doug Lauffenburger, while minoring in Molecular and Cell Biology. Finally, he conducted a postdoctoral fellowship in the laboratory of Fred Gage at the Salk Institute for Biological Studies in La Jolla, CA, before moving to UC Berkeley in 1999. At Berkeley, Dr. Schaffer applies engineering principles to enhance stem cell and gene therapy approaches for neuroregeneration. This work includes mechanistic investigation of stem cell control, as well as molecular evolution and engineering of viral gene delivery vehicles. David Schaffer has received an NSF CAREER Award, Office of Naval Research Young Investigator Award, Whitaker Foundation Young Investigator Award, and was named a Technology Review Top 100 Innovator. He was also awarded the American Chemical Society BIOT Division Young Investigator Award in 2006, the Biomedical Engineering Society Rita Shaffer Young Investigator Award in 2000, and was elected to the College of Fellows of the American Institute of Medical and Biological Engineering in 2010. </p>
<h3>Awards and Honors</h3>
<ul>
<li> Glenn Award for Research in Biological Mechanisms of Aging, 2014 </li>
<li> Distinguished Lindsey Lectureship, Texas A&M, 2012 </li>
<li> Eagleson Award, American Biological Safety Association, 2012 </li>
<li> Fellow, American Institute for Medical and Biological Engineers, 2010 </li>
<li>Robert W. Vaughan Lectureship, California Institute of Technology, 2008</li>
<li>American Chemical Society Biochemical Technology Division Young Investigator Award, 2006</li>
<li>Van Ness Award Lecturer, Rennselaer Polytechnic Institute, 2005</li>
<li>Departmental Citation for Excellence in Teaching, 2003</li>
<li>Technology Review Magazine “Top 100 Innovator Under 35,” 2002</li>
<li>American Institute of Chemical Engineers (NorCal) Excellence in Teaching Award, 2002</li>
<li>Hellman Family Faculty Award, 2001</li>
<li>Whitaker Foundation Biomedical Engineering Research Award (Young Investigator), 2001-2004</li>
<li>National Science Foundation CAREER Award, 2001-2006 </li>
<li>Office of Naval Research Young Investigator, 2001-2004 </li>
<li>The Biomedical Engineering Society Rita Shaffer Young Investigator Award, 2000 </li>
<li>Regents' Junior Faculty Award, 1999 </li>
<li>NIH Postdoctoral Fellowship (Individual NRSA), 1998 </li>
<li>MIT Department of Chemical Engineering Outstanding Seminar Award, 1997 </li>
<li>National Science Foundation Graduate Fellowship, 1993 (declined) </li>
<li>Whitaker Foundation Graduate Fellowship, 1993 (declined) </li>
<li>Fannie and John Hertz Foundation Graduate Fellowship, 1993 (accepted)</li>
</ul>
<h3>Positions and Employment</h3>
<ul>
<li>Professor, Department of Chemical Engineering, Department of Bioengineering, & Helen Wills Neuroscience Institute, U.C. Berkeley, 2007-present</li>
<li> Associate Professor, U.C. Berkeley Department of Chemical Engineering & Helen Wills Neuroscience Institute, 2005-2007</li>
<li> Assistant Professor, U.C. Berkeley Department of Chemical Engineering & Helen Wills Neuroscience Institute, 1999-2005</li>
<li>Chemist Scientist Faculty, Physical Biosciences Division, Lawrence Berkeley National Lab, 2005-present</li>
<li>Director, Berkeley Stem Cell Center, 2011-present</li>
<li>Co-Director, Berkeley Stem Cell Center, 2009-2011</li>
<li>Associate Director, Berkeley Stem Cell Center, 2007-2009</li>
</ul>
<h3>Additional Affiliations</h3>
<ul>
<li>Co-Founder, <a href="http://www.4dmoleculartherapeutics.com/" target="_blank">4D Molecular Therapeutics</a>, 2013-present</li>
<li>Editorial Board, <em>Brain Plasticity</em>, 2013-present</li>
<li>Editorial Board, <em>Technology Journal</em>, 2013-present</li>
<li>Editorial Board, <em>Molecular Therapy Nucleic Acids</em>, 2012-present</li>
<li>Scientific Advisory Board, Adheren Inc., 2012-present</li>
<li>Treasurer & Board of Directors Member, American Society for Gene and Cell Therapy (ASGCT), 2011-2014</li>
<li>Scientific Advisory Board, Cell Guidance Systems Inc., 2011-2014</li>
<li>Scientific Advisory Board, Veristem Inc., 2010-present</li>
<li>Editorial Board, <em>Molecular Therapy</em>, 2009-present</li>
<li>NIH Study Section, Biomaterials and Biointerfaces (BMBI), 2006-2010</li>
<li>Editorial Board, <em>International Journal of Neuroprotection and Neuroregeneration</em>, 2004-present</li>
<li>Advisory Board, Society for Biological Engineering, 2004-present</li>
<li>Scientific and Technology Advisory Board, Bio-Rad Laboratories, Inc., 2003-present</li>
<li>Editorial Board, <em>Biotechnology and Bioengineering</em>, 2003-present </li>
<li>Editorial Board, <em>Journal of Biotechnology and Applied Biochemistry</em>, 2002-2007</li>
</ul>
</div> <!-- /container for bio -->
<div class="container webpage" id="interests">
<div class="page-header">
<h1>Research Interests</h1>
</div>
<p>
Our research group employs molecular and cellular engineering approaches to investigate biomedical problems. We are interested in the related areas of stem cell bioengineering, gene delivery systems, and molecular virology, with applications in regenerative medicine and tissue engineering.
</p>
<h3>Stem Cells</h3>
<div class="owl-carousel">
<div class="item"><a class="group3" href="img/StemCells/11.jpg" title="Confocal image of an adult rat hippocampus, the area of the brain where new memories are formed, at 25X magnification. Astrocytes (yellow) signal to neural stem cells (white) found below the granular cell layer to differentiate into immature neurons. Cell nuclei are shown in blue. Courtesy of Anthony Conway"><img class="lazyOwl" data-src="img/StemCells/11.jpg" /></a></div>
<div class="item"><a class="group3" href="img/StemCells/22.jpg" title="Confocal image of an adult rat hippocampus, the area of the brain where new memories are formed, at 25X magnification. Astrocytes (white) signal to neural stem cells found below the granular cell layer (green) to differentiate into immature neurons (red). Courtesy of Anthony Conway"><img class="lazyOwl" data-src="img/StemCells/22.jpg" /></a></div>
<div class="item"><a class="group3" href="img/StemCells/33.jpg" title="Confocal image of an adult rat hippocampus, the area of the brain where new memories are formed, at 63X magnification. Astrocytes (green) signal to neural stem cells (white) to differentiate into immature neurons (red). Cell nuclei are shown in blue. Courtesy of Anthony Conway"><img class="lazyOwl" data-src="img/StemCells/33.jpg" /></a></div>
<div class="item"><a class="group3" href="img/StemCells/44.jpg" title="Human embryonic stem cells (red neurons, green dopaminergic neurons) bert. Courtesy of Albert Keung"><img class="lazyOwl" data-src="img/StemCells/44.jpg" /></a></div>
<div class="item"><a class="group3" href="img/StemCells/55.jpg" title="Confocal image of an adult mouse hippocampus, the area of the brain where new memories are formed, infected with lentivirus at 10X magnification. GFP+ infected neurons (green) in the granular cell layer of the dentate gyrus as well as the pyramidal cell layer project dendrites into the molecular cell layer of the hippocampus. Nuclei are shown in blue. Courtesy of Anthony Conway"><img class="lazyOwl" data-src="img/StemCells/55.jpg" /></a></div>
<div class="item"><a class="group3" href="img/StemCells/66.jpg" title="Confocal image of an adult mouse hippocampus, the area of the brain where new memories are formed, at 100X magnification. β-galactosidase is shown in red, which is driven by an inducible Nestin-Cre reporter, and thus indicates current and differentiated neural stem cells. The astrocytic marker GFAP is shown in green and Sox2 is in white. Cell nuclei are shown in blue. Courtesy of Anthony Conway"><img class="lazyOwl" data-src="img/StemCells/66.jpg" /></a></div>
<div class="item"><a class="group3" href="img/StemCells/77.jpg" title="Confocal image of an adult mouse hippocampus, the area of the brain where new memories are formed, infected with lentivirus at 100X magnification. GFP+ infected neurons (green) in the granular cell layer of the dentate gyrus project dendrites into the molecular cell layer above and axons into the hilus below. Nuclei are shown in blue. Courtesy of Anthony Conway"><img class="lazyOwl" data-src="img/StemCells/77.jpg" /></a></div>
<div class="item"><a class="group3" href="img/StemCells/Cry2Rhoa.jpg" title="3T3s expressing Cry2-RhoA and costained for Lifeact-Venus"><img class="lazyOwl" data-src="img/StemCells/Cry2Rhoathumb.jpg" /></a></div>
<div class="item"><a class="group3" href="img/StemCells/humancortical.jpg" title="Alzheimer’s disease human in vitro model: human cortical cultures (green) treated with amyloid beta oligomers (red)."><img class="lazyOwl" data-src="img/StemCells/humancorticalthumb.jpg" /></a></div>
</div>
<p>
Many of our efforts are dedicated to understanding the biology and exploring the therapeutic potential of stem cells. Stem cells are immature cells that exist in various locations of our bodies. Throughout our lifetimes, these cells divide and develop into the specialized cells that perform the functions necessary for life. Therefore, if we contract a disease that kills those specialized cells, our stem cells are a potential source for replacing lost cells to counteract or even cure the disorder. There are several challenges that must be overcome in this field. In particular, efforts to engineer tissues rely upon the ability to control stem cells. That is, the signals that control stem cell function and fate must first be discovered, and then integrated into cellular microenvironments to control stem cell expansion and lineage-specific differentiation. We have efforts in novel signal discovery, computational and experimental analysis of the biological networks that cells use to interpret and implement these signals, and on the integration of these signals into synthetic, polymeric microenvironments for optimal stem cell control in collaboration with the group of Prof. Kevin Healy (Bioengineering). This blend of stem cell biology, systems biology analysis, and biomaterials engineering has led to significant advances in the application of stem cells for tissue repair.<br />
<br />
Researchers: Tandis, Maroof, Goncalo, Barbara, Alyssa, Nicole, Anusuya, Sisi, Dawn, Jorge, Mike, Phillip, Olivia, Andrew, Sean, Brian, Alyssa and Riya
</p>
<h3>Gene Therapy</h3>
<div class="owl-carousel">
<div class="item"><a class="group1" href="img/GeneTherapy/7m8scCAGGFPinbalbcRPE.jpg" title="A new variant of AAV, called 7m8, was created through directed evolution, and has the ability to infect the outer retina from the vitreous. This image shows GFP expression in the retinal pigment epithelium of a mouse injected with 7m8-GFP."><img class="lazyOwl" data-src="img/GeneTherapy/7m8scCAGGFPinbalbcRPEthumb.jpg" /></a></div>
<div class="item"><a class="group1" href="img/GeneTherapy/40Xsnappopcorn.jpg" title="Through directed evolution of AAV, we created a new variant that has the ability to infect photoreceptors from the vitreous. In this image of a primate retina, bright GFP expression is seen around a retinal blood vessel. The inner limiting membrane is labelled in red. Blue is DAPI labeled nuclei. Green is native GFP expression."><img class="lazyOwl" data-src="img/GeneTherapy/40Xsnappopcornthumb.jpg" /></a></div>
<div class="item"><a class="group1" href="img/GeneTherapy/slicefrom40XstackPNA.jpg" title="Through directed evolution, we created a new AAV variant that has the ability to infect photoreceptors from the vitreous. This image shows GFP expression in photoreceptors from a primate retina injected intravitreally with 7m8-GFP. Red is PNA labeled cones. Green is native GFP expression."><img class="lazyOwl" data-src="img/GeneTherapy/slicefrom40XstackPNAthumb.jpg" /></a></div>
<div class="item"><a class="group1" href="img/GeneTherapy/WTDAPIsyn.jpg" title="WT mouse retina. Green is labeling of synaptophysin, a presynaptic marker. Blue is DAPI labeling of nuclei in the GCL, inner nuclear layer and outer nuclear layers."><img class="lazyOwl" data-src="img/GeneTherapy/WTDAPIsynthumb.jpg" /></a></div>
</div>
<p>
Our second major research thrust is dedicated to understanding the biology and exploring the therapeutic potential of gene delivery, which serves as an effective means to control stem cells. Gene therapy can be defined as the introduction of genetic material to the cells of an individual for therapeutic benefit. A variety of approaches are under development to use gene therapy for treating cancer, AIDS, and a number of inherited genetic disorders. For example, gene therapy could be used to replace the genes hemophilia patients are missing, to bolster the immune system to recognize and combat tumors, or to inhibit the replication of HIV virus. However, significant progress must still be made before these developing strategies become therapeutic realities. One of the most formidable obstacles to gene therapy is how to efficiently deliver genes to a sufficient number of cells to yield a therapeutic effect. A number of gene delivery vehicles, or vectors, are in development, and most exploit or emulate the abilities many viruses have evolved to deliver their genes to cells as part of their life cycles. However, while viruses have developed numerous strategies to deliver genes over millions of years of evolution, the efficiency and safety of vehicles based upon recombinant viruses must still be further improved. We have developed numerous high-throughput directed evolution approaches to engineer the properties of viral vehicles at the molecular level to enhance their abilities to deliver genes. These successful efforts are enhancing the abilities of several vectors to make them more effective at delivering gene "medicines."<br />
<br />
Researchers: David, Maroof, Sabrina, Leah, Mervi, Tim, Jonathan, Benjamin and Thom
</p>
<h3>HIV</h3>
<section class="clearfix">
<img class="regular" src="img/hiv2.jpg" alt="Maximum Intensity Projection (MIP) of a field of a clonal population of Jurkat T cells bearing a single integration of
a model HIV LTR reporter system. Single molecular RNA Fluorescent-In-Situ-Hybridization permits single cell resolution
of transcript number and direct inference of transcriptional dynamics. Significant heterogeneity in RNA number is observed in many clonal populations and is suggestive of an underlying bursty transcriptional process. Credit: Jonathan Foley" width="276px">
<p>
In parallel, we are interested in studying some basic aspects of viral biology. Specifically, viruses have evolved gene circuits that after infecting a cell execute programs to harness cells to reproduce the virus. We apply integrated systems biology approaches, composed of computational and experimental efforts, in collaboration with the group of Prof. Adam Arkin (Bioengineering) to how the structures of these gene circuits have dynamically evolved to optimize the virus' ability to hijack cells to maximize its ability to reproduce. This fundamental work is leading to new insights on how to combat viral infectious disease.<br />
<br />
Researchers: Jayodita and Prajit
</p>
</section>
<h3>Future Goals</h3>
<p>
We plan for these related lines of research to converge in the future. If we can effectively deliver a gene, and we can learn much more about what kinds and levels of genes are needed to control stem cell behavior, we can attempt to apply this information to longer-term therapeutic goals. These aims could include using gene delivery to stimulate stem cells to divide more rapidly, to generate specific types of cells such as neurons, or to guide the successful integration of specific cell types into tissue for functional repair. It is our hope that this research will not only enhance our understanding of neuroscience, but also eventually alleviate the devastating effects of numerous diseases.
</p>
</div> <!-- /container for interests -->
<div class="container webpage" id="ourgroup">
<div class="page-header">
<h1>Our Research Group</h1>
</div>
<img class="center" src="img/coverphoto.jpg" alt="" width="600px"></img>
<div class="row" width="100%">
<div class="col-xs-6">
<img class="center" name="" src="img/SCHAFFERSKI.jpg" alt="">
<p class="center">
David V. Schaffer, Ph.D.<br />
Principal Investigator<br />
<a href="mailto:[email protected]">[email protected]</a>
</p>
</div>
<div class="col-xs-6">
<img class="center" name="" src="img/NOEMSKI.jpg" alt="">
<p class="center">
Wanichaya Ramey<br />
Lab Manager/Administrator<br />
<a href="mailto:[email protected]">[email protected]</a>
</p>
</div>
</div>
<h3>Graduate Students</h3>
<div id="gradstudents"></div>
<h3>Research Scientists</h3>
<div id="scientists"></div>
<h3>Postdocs</h3>
<div id="postdocs"></div>
<h3>Visiting Scholars</h3>
<div id="scholars"></div>
<h3>Undergraduate Work-Study Students</h3>
<div id="undergrads"></div>
<h3>Lab Alumni</h3>
<div id="alumni"></div>
</div> <!-- /container for ourgroup -->
<div class="container webpage" id="publications">
<div class="page-header">
<h1>Publications</h1>
</div>
<p align="right">
<a href="http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=DetailsSearch&term=schaffer+dv&log$=activity" target="new">Search for Publication</a>
</p>
<div id="accordion">
<h3>Recent Selected Publications</h3>
<div>
<ul>
<li>Kotterman, M.A. and D.V. Schaffer “Engineering Adeno-Associated Viruses for Clinical Gene Therapy.” <em>Nature Reviews Genetics</em>, 15:445-451 (PMID 24840552). <a href="2014 Publications/Kotterman_AAVprogress_NRG_14.pdf" target= "_blank">View PDF</a>
<li>Hughes, A.J., D.P. Spelke, Z. Xu, C-C. Kang, D.V. Schaffer (co-corresponding author), and A.E. Herr (2014) “Single-cell Western Blotting.” <em>Nature Methods</em>, 11(7):749-55 (PMID 24880876). <a href="2014 Publications/HughesSpelke_SCW_NatMeth_14.pdf" target= "_blank">View PDF</a>
<li>Shapiro, M., P. Goodwill, A. Neogy, M. Yin, F. Foster, D.V. Schaffer, and S. Conolly (2014) “Biogenic Gas Nanostructures as Ultrasonic Molecular Reporters.” <em>Nature Nanotechnology</em>, 9(4):311-6 (PMID: 24633522). <a href="2014 Publications/Shapiro_GVultrasound_NNano_14.pdf" target= "_blank">View PDF</a>
<li>Shapiro, M.G., R.M. Ramirez, L.J. Sperling, G. Sun, A. Pines, D.V. Schaffer, and V.S. Bajaj “Genetically Encoded Reporters for Hyperpolarized Xenon.” <em>Nature Chemistry</em>, 6:629-634 (PMID 24950334). <a href="2014 Publications/Shapiro_GVforXe_NatChem_14.pdf" target= "_blank">View PDF</a>
<li>Lei, Y. and D.V. Schaffer (2013) “A Fully Defined and Scalable 3D Culture System for Human Pluripotent Stem Cell Expansion and Differentiation.” <em>Proceedings of the National Academy of Sciences USA</em>, 110:E5039-5048 (PMID 24248365). <a href="2013 Publications/Lei_3DmaterialhESCs_PNAS_13.pdf" target= "_blank">View PDF</a>
<li>Conway, A., T. Vazin, D.P. Spelke, N.A. Rode, K.E. Healy, R.S. Kane, and D.V. Schaffer (2013) “Multivalent Ligands to Control Stem Cell Behaviour in Vitro and in Vivo.” <em>Nature Nanotechnology</em>, 8:831-838 (PMID 24141540). <a href="2013 Publications/Conway_EphrinConj_NNano_13.pdf" target= "_blank">View PDF</a>
<li>Downing, T.L., J. Soto, C. Morez, T. Houssin, A. Fritz, F. Yuan, J. Chu, S. Patel, D.V. Schaffer, and S. Li (2013) “Biophysical Regulation of Epigenetic State and Cell Reprogramming.” <em>Nature Materials</em>, 12:1154-1162 (PMID 24141451). <a href="2013 Publications/Downing_iPStopography_NMats_13.pdf" target= "_blank">View PDF</a>
<li>Dalkara, D., L.C. Byrne, R.R. Klimczak, M. Visel, L. Yin, W.H. Merigan, J.G. Flannery, and D.V. Schaffer (2013) "In Vivo–Directed Evolution of a New Adeno-Associated Virus for Therapeutic Outer-Retinal Gene Delivery from the Vitreous." <em>Science Translational Medicine</em>, 5:189ra76 (PMID 23761039). <a href="2013 Publications/Dalkara_Retina7m8_STM_13.pdf" target= "_blank">View PDF</a>
<li>Bugaj, L.J, A.T Choksi, C.K. Mesuda, R.S. Kane, and D.V. Schaffer (2013) "Optogenetic Protein Clustering and Signaling Activation in Mammalian Cells." <em>Nature Methods</em>, 10:249-252. <a href="2013 Publications/Bugaj_Cry2Opto_NMeth_13.pdf" target= "_blank">View PDF</a>
<li>Ashton, R.S., A. Conway, C. Pangarkar, J. Bergen, K-I. Lim, P. Shah, M. Bissell, and D.V. Schaffer (2012) "Astrocytes Regulate Adult Hippocampal Neurogenesis Through Ephrin-B Signaling." <em>Nature Neuroscience</em>,15:1399-1406 (PMID: 22983209). <a href="2012 Publications/Astrocytes Regulate Adult Hippocampal Neurogenesis Through Ephrin-B Signaling.pdf" target= "_blank">View PDF</a>
<li> Dickinson, B.C., J. Peltier, D. Stone, D.V. Schaffer (co-corresponding author), and C.J Chang (2011) "Nox2 Redox Signaling Maintains Essential Cell Populations in the Brain." <em>Nature Chemical Biology</em>, 7:106-112 (PMID 21186346). <a href="2011 Publications/nox2_schaffer.pdf" target= "_blank">View PDF</a>
</li>
<li>Lim, K-I., R.R. Klimczak, J.H. Yu, and D.V. Schaffer (2010) "Specific Insertions of Zinc Finger Domains into Gag-Pol Yields Engineered Retroviral Vectors with Selective Integration Properties." <em>Proceedings of the National Academy of Sciences USA</em>, 107:12475-12480 (PMID 20616052). <a href="2010 Publications/Lim_ZFDretroviralIntegration_PNAS_10.pdf" target="_blank">View PDF</a></li>
<li>Excoffon, K.J., J.T. Koerber, D.D. Dickey, M. Murtha, S. Keshavjee, B.K. Kaspar, J. Zabner, and D.V. Schaffer (2009) "Directed Evolution of Adeno-Associated Virus to an Infectious Respiratory Virus." <em>Proceedings of the National Academy of Sciences USA</em>, 106:3865-3870 (PMID 19237554). <a href="2008 Publications/Excoffon_AAVlung_PNAS_09.pdf" target="_blank">View PDF</a></li>
<li>Koerber, J.T., N. Maheshri, B.K. Kaspar, and D.V. Schaffer (2006) "Construction of Diverse Adeno-Associated Viral Libraries for Directed Evolution of Enhanced Gene Delivery Vehicles." <em>Nature Protocols</em>, 1:701-706. <a href="2006 Publications/Koerber_NatProtocols_06.pdf" target="_blank">View PDF</a></li>
<li>Maheshri, N., J.T. Koerber, B. Kaspar, and D.V. Schaffer (2006) "Directed Evolution of Adeno-Associated Virus Yields Enhanced Gene Delivery Vectors." <em>Nature Biotechnology</em>, 24:198-204. <a href="2006 Publications/Maheshri_AAVevo_NBT_06.pdf" target="_blank">View PDF</a></li>
<li>Weinberger, L.S., J.C. Burnett, J.E. Toettcher, A.P. Arkin, and D.V. Schaffer (2005) "Stochastic Gene Expression in a Lentiviral Feedback Loop: HIV-1 Tat Expression Drives Phenotypic Diversity." <em>Cell</em>, 122:169-182. <a href="2005 Publications/Weinberger_Cell_05.pdf" target="_blank">View PDF</a></li>
<li>Maheshri, N. and D.V. Schaffer (2003) "Computational and Experimental Analysis of DNA Shuffling." <em>Proceedings of the National Academy of Sciences USA</em>, 100:3071-3076. <a href="2003 Publications/Maheshri_PNAS_03.pdf" target="_blank">View PDF</a></li>
<li>Lai, K, B.K. Kaspar, F.H. Gage, and D.V. Schaffer (2003) "Sonic Hedgehog Regulates Adult Neural Progenitor Proliferation in Vitro and in Vivo." <em>Nature Neuroscience</em>, 6(1):21-27. <a href="2003 Publications/Lai_ShhNeurogen_NN_03.pdf" target="_blank">View PDF</a></li>
</ul>
</div>
<h3>Recent Review Articles</h3>
<div>
<ul>
<li>Kotterman, M.A. and D.V. Schaffer (2014)“Engineering Adeno-Associated Viruses for Clinical Gene Therapy.” <em>Nature Reviews Genetics</em>, 445-51 (PMID: 24840552). <a href="2014 Publications/Kotterman_AAVprogress_NRG_14.pdf" target= "_blank">View PDF</a>
<li>Asokan, A., D.V. Schaffer, and R.J. Samulski (2012) "The AAV Vector Toolkit: Poised at the Clinical Crossroads." <em>Molecular Therapy</em>, 20:699-708 (PMID 22273577). <a href="2012 Publications/Publication number 4.pdf" target= "_blank">View PDF</a>
<li>Bartel, M.A., J.R. Weinstein, and D.V. Schaffer (2012) "Directed Evolution of Novel Adeno-Associated Viruses for Therapeutic Gene Delivery." <em>Gene Therapy</em>, 19:694-700 (PMID 22402323). <a href="2012 Publications/Publication number 6.pdf" target= "_blank">View PDF</a></li>
<li>Arkin, A.P. and D.V. Schaffer (2011) "Network News: Innovations in 21st Century Systems Biology." <em>Cell</em>, 144:844-849 (PMID 21414475). <a href="2011 Publications/Arkin Schaffer.pdf" target= "_blank">View PDF</a></li>
<li>Keung, A.J., S. Kumar, and D.V. Schaffer (2010) "Presentation Counts: Microenvironmental Regulation of Stem Cells by Biophysical and Material Cues." <em>Annual Reviews in Cell and Developmental Biology</em>, 26:533-556 (PMID 20590452). <a href="2010 Publications/Keung_NicheReview_ARCDB_10.pdf" target="_blank">View PDF</a></li>
<li>Vazin, T. and D.V. Schaffer (2010) "Engineering Strategies to Emulate the Stem Cell Niche Trends in Biotechnology." <em>Trends in Biotechnology</em>, 28:117-124 (PMID 20042248). Selected as the Cover Article. <a href="2010 Publications/Vazin_NicheReview_TibTech_10.pdf" target="_blank">View PDF</a></li>
</ul>
</div>
<h3>2015</h3>
<div>
<ul>
<li>Ojala, D., D.P. Amara, and D.V. Schaffer (2015) “AAV Vectors and Neurological Gene Therapy.” Neuroscientist, 21(1): 84-98. <a href="2015 Publications/3.pdf" target= "_blank">View PDF</a>
<li>Fritz, A.L., S.R. Mao, M.G. West, and D.V. Schaffer (2015) “A Medium-Throughput Analysis of Signaling Pathways Involved in Early Stages of Stem Cell Reprogramming.” Biotechnology & Bioengineering, 112 (1): 209-19 (PMID 25065366). <a href="2015 Publications/4.pdf" target= "_blank">View PDF</a>
<li>Byrne, L.C., Y.J. Lin, T. Lee, D.V. Schaffer, and J.G. Flannery (2015) “The Expression Pattern of Systemically Injected AAV9 in the Developing Mouse Retina is Determined by Age.” Molecular Therapy, 23(2): 290-6 (PMID 25224467). <a href="2015 Publications/5.pdf" target= "_blank">View PDF</a>
<li>Kotterman, M.A., L. Yin, J.M. Strazzeri, J.G. Flannery, W.M. Merigan, and D.V. Schaffer (2015) “Antibody Neutralization Poses a Barrier to Intravitreal Adeno-Associated Viral Vector Gene Delivery to Non-Human Primates.” Gene Therapy, 22(2):116-26 (PMID 25503696). <a href="2015 Publications/6.pdf" target= "_blank">View PDF</a>
<li>Yousef, H., A. Morgenthaler, C. Schlesinger, L. Bugaj, I. Conboy, and D.V. Schaffer (2015) “Age-Associated Increase in BMP Signaling inhibits Hippocampal Neurogenesis.” Stem Cells (in press). <a href="2015 Publications/7.pdf" target= "_blank">View PDF</a>
<li>Jha, A.K., K.M. Tharp, J. Ye, J.L. Santiago-Ortiz, W.M. Jackson, A. Stahl, D.V. Schaffer, Y. Yeghiazarians, and K.E. Healy (2015)“Enhanced Survival and Engraftment of Transplanted Stem Cells Using Growth Factor Sequestering Hydrogels.” Biomaterials, 47:1-12 (PMID 25682155). <a href="2015 Publications/8.pdf" target= "_blank">View PDF</a>
<li>Schaffer, D.V. (corresponding author), M.A. Kotterman, and T.W. Chalberg (2015) “Viral Vectors for Gene Therapy: Translational and Clinical Outlook.” Annual Review of Biomedical Engineering (in press).
<li>Bugaj, L.J., D. P. Spelke, C.K. Mesuda, M. Varedi, R.S. Kane, and D.V. Schaffer (2015)“Regulation of Endogenous Transmembrane Receptors Through Optogenetic Cry2 Clustering.” Nature Communications (in press).
<li>Kotterman, M.A., T. Vazin, and D.V. Schaffer (2015) “Adeno-Associated Viral Variant Enhances Selective Gene Delivery to Neural Stem Cells in Vivo.” Development (in press).
<li>Dey, S.S., J.E. Foley, P. Limsirichai, D.V. Schaffer (co-corresponding author), and A.P. Arkin (2015) “Orthogonal Control of Expression Mean and Variance by Epigenetic Features at Different Genomic Loci” Molecular Systems Biology (in press).
</ul>
</div>
<h3>2014</h3>
<div>
<ul>
<li>Conway A., D.P. Spelke, and D.V. Schaffer (2014) “Conjugation of Proteins to Polymer Chains to Create Multivalent Molecules.” <em>Methods in Molecular Biology</em>, 1202:95-102 (PMID 24014311). <a href="2014 Publications/1.pdf" target= "_blank">View PDF</a>
<li>Hughes, A.J., D.P. Spelke, Z. Xu, C-C. Kang, D.V. Schaffer (co-corresponding author), and A.E. Herr “Single-cell Western Blotting.” <em>Nature Methods</em>, 11:749-755 (PMID 24880876). <a href="2014 Publications/HughesSpelke_SCW_NatMeth_14.pdf" target= "_blank">View PDF</a>
<li>Kotterman, M.A. and D.V. Schaffer “Engineering Adeno-Associated Viruses for Clinical Gene Therapy.” <em>Nature Reviews Genetics</em>, 15:445-451 (PMID 24840552). <a href="2014 Publications/Kotterman_AAVprogress_NRG_14.pdf" target= "_blank">View PDF</a>
<li>Shapiro, M.G., R.M. Ramirez, L.J. Sperling, G. Sun, A. Pines, D.V. Schaffer, and V.S. Bajaj (2014) “Genetically Encoded Reporters for Hyperpolarized Xenon.” <em>Nature Chemistry</em>, 6(7):629-34 (PMID: 24950334). <a href="2014 Publications/Shapiro_GVforXe_NatChem_14.pdf" target= "_blank">View PDF</a>
<li>Shapiro, M., P. Goodwill, A. Neogy, M. Yin, F. Foster, D.V. Schaffer, and S. Conolly “Biogenic Gas Nanostructures as Ultrasonic Molecular Reporters.” <em>Nature Nanotechnology</em>, 9:311-316 (PMID 24633522). <a href="2014 Publications/Shapiro_GVultrasound_NNano_14.pdf" target= "_blank">View PDF</a>
<li>Vazin, T., R.S. Ashton, A. Conway, N. Rode, S.M. Lee, V. Bravo, K.E. Healy, R.S. Kane, and D.V. Schaffer (2014) “The Effect of Multivalent Sonic Hedgehog on Differentiation of Human Embryonic Stem Cells into Dopaminergic and GABAergic Neurons.” <em>Biomaterials</em>, 35:941-948 (PMID 24172856). <a href="2014 Publications/Vazin_MultivalentShh_Biomats_14.pdf" target= "_blank">View PDF</a>
<li>Vacca, O., M. Darche, D.V. Schaffer, J.G. Flannery, J.A. Sahel, A. Rendon, and D. Dalkara (2014) “AAV-mediated Gene Delivery in Dp71-null Mouse Model with Compromised Barriers.” <em>Glia</em>, 62:468-476 (PMID 24382652). <a href="2014 Publications/Dalkara_Dp71Shh10_Glia_14.pdf" target= "_blank">View PDF</a>
<li>Ojala, D., D.P. Amara, and D.V. Schaffer “AAV Vectors and Neurological Gene Therapy.” <em>Neuroscientist</em> (in press). <a href="2014 Publications/Ojala_AAVCNSreview_Neurosci_14.pdf" target= "_blank">View PDF</a>
<li>Conway, A. and D.V. Schaffer “Biomaterial Microenvironments to Support the Generation of New Neurons in the Adult Brain.” <em>Stem Cells</em>, 32:1220-1229 (PMID 24449485). <a href="2014 Publications/Conway_BiomatsInSitu_StemCells_14.pdf" target= "_blank">View PDF</a>
<li>Bartel, M.A. and D.V. Schaffer (2014) “Enhanced Gene Targeting of Adult and Pluripotent Stem Cells Using Evolved Adeno-Associated Virus.” <em>Methods in Molecular Biology</em>, 1114: 169-179 (PMID 24557903). <a href="2014 Publications/Bartel_GeneCorrection_MIMB_14.pdf" target= "_blank">View PDF</a>
<li>Loring, J.F., T.C. McDevitt, S.P. Palecek, D.V. Schaffer, P.W. Zandstra, and R.M. Nerem (2014) “A Global Assessment of Stem Cell Engineering.” <em>Tissue Engineering Part A</em>, 20(19-20):2575-89 (PMID 24428577). <a href="2014 Publications/2.pdf" target= "_blank">View PDF</a>
<li>Schaffer, D.V. (2014) “AAV Shuffles to the Liver.” <em>Molecular Therapy – Methods & Clinical Development</em>, 14006. <a href="2014 Publications/Schaffer_Cmtry_MTMCD_14.pdf" target= "_blank">View PDF</a>
<li>Day, T.P., L.C. Byrne, D.V. Schaffer, and J.G. Flannery (2014) “Advances in AAV Vector Development for Gene Therapy in the Retina.” <em>Advances in Experimental Medicine and Biology</em>, 801:687-693 (PMID 24664759). <a href="2014 Publications/Dey_RetinalGTReview_AEMB_14.pdf" target= "_blank">View PDF</a>
<li>Yin, L., B. Masella, D. Dalkara, J. Zhang, J.G. Flannery, D.V. Schaffer, D.R. Williams, and W.H. Merigan (2014) “Imaging Light Responses of Foveal Ganglion Cells in the Living Macaque Eye.” <em>Journal of Neuroscience</em>, 7;34 (19) (PMID: 24806684). <a href="2014 Publications/Merigan_RGCGCAMP_JN_14.pdf" target= "_blank">View PDF</a>
<li>Leo, Y., D. Jeong, J, Xiao, and D.V. Schaffer (2014) “Developing Defined and Scalable 3D Culture Systems for Culturing Human Pluripotent Stem Cells at High Densities.” <em>Cell and Molecular Bioengineering</em>, Volume 7, Issue 2, pp 172-183 <a href="2014 Publications/Springer_14.pdf" target= "_blank">View PDF</a>
<li>Byrne, L.C., B.E. Öztürk, T. Lee, C. Fortuny, M. Visel, D. Dalkara, D.V. Schaffer, and J.G. Flannery (2014)“Retinoschisin Gene Therapy in Photoreceptors Provides Long-term Rescue in the Rs1h-/- Mouse.” <em>Gene Therapy</em>, 21(6): 585-92 (PMID: 24694538). <a href="2014 Publications/Byrne_RsAAV_GT_14.pdf" target= "_blank">View PDF</a>
<li>Cao, J., M. Ahra, C. Sudrik, D.V. Schaffer, and R.S. Kane (2014)“Bidirectional Regulation of mRNA Translation in Mammalian Cells by Using PUF Domains.” <em>Angewandte Chemie</em>, 5;53 (19):4900-4 (PMID: 24677733). <a href="2014 Publications/Cao_PUFmRNA_Angewandte_14.pdf" target= "_blank">View PDF</a>
</ul>
</div>
<h3>2013</h3>
<div>
<ul>
<li>Lei, Y. and D.V. Schaffer (2013) “A Fully Defined and Scalable 3D Culture System for Human Pluripotent Stem Cell Expansion and Differentiation.” <em>Proceedings of the National Academy of Sciences USA</em>, 110:E5039-5048 (PMID 24248365). <a href="2013 Publications/Lei_3DmaterialhESCs_PNAS_13.pdf" target= "_blank">View PDF</a>
<li>Conway, A., T. Vazin, D.P. Spelke, N.A. Rode, K.E. Healy, R.S. Kane, and D.V. Schaffer (2013) “Multivalent Ligands to Control Stem Cell Behaviour in Vitro and in Vivo.” <em>Nature Nanotechnology</em>, 8:831-838 (PMID 24141540). <a href="2013 Publications/Conway_EphrinConj_NNano_13.pdf" target= "_blank">View PDF</a>
<li>Downing, T.L., J. Soto, C. Morez, T. Houssin, A. Fritz, F. Yuan, J. Chu, S. Patel, D.V. Schaffer, and S. Li (2013) “Biophysical Regulation of Epigenetic State and Cell Reprogramming.” <em>Nature Materials</em>, 12:1154-1162 (PMID 24141451). <a href="2013 Publications/Downing_iPStopography_NMats_13.pdf" target= "_blank">View PDF</a>
<li>Dalkara, D., L.C. Byrne, R.R. Klimczak, M. Visel, L. Yin, W.H. Merigan, J.G. Flannery, and D.V. Schaffer (2013) "In Vivo–Directed Evolution of a New Adeno-Associated Virus for Therapeutic Outer-Retinal Gene Delivery from the Vitreous." <em>Science Translational Medicine</em>, 5:189ra76 (PMID 23761039). <a href="2013 Publications/Dalkara_Retina7m8_STM_13.pdf" target= "_blank">View PDF</a>
<li>Miller-Jensen, K., R. Skupsky, P.S. Shah, A.P. Arkin, and D.V. Schaffer (2013) “Genetic Selection for Context-Dependent Stochastic Phenotypes: Sp1 and TATA Mutations Increase Phenotypic Noise in HIV-1 Gene Expression.” <em>Public Library of Science (PLoS) Computational Biology</em>, 9:e1003135 (PMID 23874178). <a href="2013 Publications/Miller-Jensen_Stoch_PLoSCompBio_13.pdf" target= "_blank">View PDF</a>
<li>Yousef, H., M.J. Conboy, J. Li, M. Zeiderman, T. Vazin, C. Schlesinger, D.V. Schaffer, and I.M. Conboy (2013) “hESC-Secreted Proteins can be Enriched for Multiple Regenerative Therapies by Heparin-Binding.” <em>Aging</em>, 5:357-372 (PMID 23793469). <a href="2013 Publications/Dalkara_Retina7m8_STM_13.pdf" target= "_blank">View PDF</a>
<li>Keung, A.J., M. Dong, D.V. Schaffer (co-corresponding author), and S. Kumar (2013) "Pan-neuronal Maturation But Not Neuronal Subtype Differentiation of Adult Neural Stem Cells is Mechanosensitive." <em>Scientific Reports</em>, 3:1817 (PMID 23660869). <a href="2013 Publications/Keung_NSCmaturationStiff_SR_13" target= "_blank">View PDF</a>
<li>Bugaj, L.J, A.T Choksi, C.K. Mesuda, R.S. Kane, and D.V. Schaffer (2013) "Optogenetic Protein Clustering and Signaling Activation in Mammalian Cells." <em>Nature Methods</em>, 10:249-252. <a href="2013 Publications/Bugaj_Cry2Opto_NMeth_13.pdf" target= "_blank">View PDF</a>
<li>Hwang, B-Y. and D.V. Schaffer (2013) “Engineering a Serum-resistant and Thermostable Vesicular Stomatitis Virus G Glycoprotein for Pseudotyping Retroviral and Lentiviral Vectors." <em>Gene Therapy</em>, 20:807-815 (PMID 23364315). <a href="2013 Publications/Hwang_VSVGevo_GT_13.pdf" target= "_blank">View PDF</a>
<li>Chen, S. and D.V. Schaffer (2013) “Newborn Neuroblasts Feel the Field in the Adult Brain.” <em>EMBO Reports</em>, 14:105-106 (PMID 23328741). <a href="2013 Publications/Chen_SVZmig_EMBOrep_13.pdf" target= "_blank">View PDF</a>
<li>Pernet, V., S. Joly, D. Dalkara, N. Jordi, O. Schwarz, F. Christ, D.V. Schaffer, J.G. Flannery, and M.E. Schwab (2013) “Long-distance Axonal Regeneration Induced by CNTF Gene Transfer is Impaired by Axonal Misguidance in the Injured Adult Optic Nerve.” <em>Neurobiology of Disease</em>, 51:202-13 (PMID 23194670). <a href="2013 Publications/Pernet_CNTF_NBD_13.pdf" target= "_blank">View PDF</a>
<li>Sudrik C., M. Arha, J. Cao, D.V. Schaffer (co-corresponding author), and R.S. Kane (2013) “Translational Repression Using BIV Tat peptide-TAR RNA Interaction in Mammalian Cells.” <em>Chemical Communications</em>, 49(67):7457-7459 (PMID 23852014). <a href="2013 Publications/Sudrik_TatTARRepr_ChemComm_13.pdf" target= "_blank">View PDF</a>
<li>Cao, J., M. Arha, C. Sudrik, L.J. Bugaj, D.V. Schaffer (co-corresponding author), and R.S. Kane (2013) “Light-inducible Activation of Target mRNA Translation in Mammalian Cells” <em>Chemical Communications</em>, 49:8338-8340 (PMID 23925486). <a href="2013 Publications/Cao_LightTranslation_ChemComm_13.pdf" target= "_blank">View PDF</a>
<li>Perry, S.J., S.G. Neumann, T. Neumann, K. Cheng, J. Ni, J.R. Weinstein, D.V. Schaffer, and M. Tirrell “Challenges in Nucleic Acid-Lipid Films for Transfection” <em>AIChE Journal</em> (in press).
<li>Byrne, L.C., F. Khalid, T. Lee, E.A. Zin, K.P. Greenberg, M. Visel, D.V. Schaffer, and J.G. Flannery (2013) “AAV-mediated, Optogenetic Ablation of Müller Glia Leads to Structural and Functional Changes in the Mouse Retina.” <em>Public Library of Science (PLoS) ONE</em>, 8:e76075 (PMID 24086689). <a href="2013 Publications/VByrne_KillerRedGlia_PLoSONE_13.pdf" target= "_blank">View PDF</a>
<li>Vazin, T., K.A. Ball, H. Lu, H. Park, Y. Ataeijannati, T. Head-Gordon, M-M Poo, and D. V. Schaffer (2013) “Efficient Derivation of Cortical Glutamatergic Neurons from Human Pluripotent Stem Cells: A Model System to Study Neurotoxicity in Alzheimer’s Disease.” <em>Neurobiology of Disease</em>, 62C:62-72 (PMID 24055772). <a href="2013 Publications/Vazin_CorticalDiff_NBD_13.pdf" target= "_blank">View PDF</a>
<li>Ekerdt, B.L., R.A. Segalman, and D.V. Schaffer (2013) “Spatial Organization of Cell-Adhesive Ligands for Advanced Cell Culture.” <em>Biotechnology Journal</em>, 8:1411-1423 (PMID 24318636).
</ul>
</div>
<h3>2012</h3>
<div>
<ul>
<li>Ashton, R.S., A. Conway, C. Pangarkar, J. Bergen, K-I. Lim, P. Shah, M. Bissell, and D.V. Schaffer (2012) "Astrocytes Regulate Adult Hippocampal Neurogenesis Through Ephrin-B Signaling." <em>Nature Neuroscience</em>, 15:1399-1406 (PMID: 22983209). <a href="2012 Publications/Astrocytes Regulate Adult Hippocampal Neurogenesis Through Ephrin-B Signaling.pdf" target= "_blank">View PDF</a>
<li>Dalkara, D., L.C. Byrne, T. Lee, N.V. Hoffmann, D.V. Schaffer, and J.G. Flannery (2012) Enhanced Gene Delivery to the Neonatal Retina Through Systemic Administration of Tyrosine-Mutated AAV9." <em>Gene Therapy</em>, 19:176-181 (PMID: 22011645). <a href="2012 Publications/Enhanced Gene Delivery to the Neonatal Retina Through Systemic Administration of Tyrosine-Mutated AAV9.pdf" target= "_blank">View PDF</a> </li>
<li>Keung, A.J., P. Asuri, S. Kumar, and D.V. Schaffer (2012) "Soft Microenvironments Promote the Early Neurogenic Differentiation but not Self-renewal of Human Pluripotent Stem Cells." <em>Integrative Biology</em>, 4(9):1049-1058 (PMID: 22854634). <a href="2012 Publications/Soft Microenvironments Promote the Early Neurogenic Differentiation but not Self-renewal of Human Pluripotent Stem Cells.pdf" target= "_blank">View PDF</a>
<li>Goodwill, P.W., E.U. Saritas, L.R. Croft, T.N. Kim, K.M. Krishnan, D.V. Schaffer, and S.M. Conolly (2012) “X-space MPI: Magnetic Nanoparticles for Safe Medical Imaging.” <em>Advanced Materials</em>, 24:3870-3877 (PMID 22988557). <a href="2012 Publications/Goodwill_MPI_AdvMat_12.pdf" target= "_blank">View PDF</a>
<li>Conway, A. and D.V. Schaffer (2012) “Biophysical Regulation of Stem Cell Behavior in the Niche.” <em>Stem Cell Research and Therapy</em>, 3:50 (PMID 23241436). <a href="2012 Publications/Conway_NicheRev_SCRT_12.pdf" target= "_blank">View PDF</a>
<li>Bugaj, L.J. and D.V. Schaffer (2012) "Bringing Next-Generation Therapeutics to the Clinic Through Synthetic Biology." <em>Current Opinion in Chemical Biology</em>, 16(3-4):355-361 (PMID: 22609142). <a href="2012 Publications/Bringing Next-Generation Therapeutics to the Clinic Through Synthetic Biology.pdf" target= "_blank">View PDF</a>
<li>Pollock, J., R. Aston, N. Rode, D.V. Schaffer, K.E. Healy (2012) "Molecular Characterization of Multivalent Bioconjugates by Size-Exclusion Chromatography (SEC) with Multi-Angle Laser Light Scattering (MALS)." <em>Bioconjugate Chemistry</em>, 19;23(9):1794-801 (PMID: 22794081). <a href="2012 Publications/Pollock_SECMALS_BC_12.pdf" target= "_blank">View PDF</a>
<li> Ana, H.J, P. Gip, J. Kimc, S. Wua, C.T. McVaugh, D.V. Schaffer, C.R. Bertozzi, and C.B. Lebirlla (2012) "N-Glycan Profiling of Human Embryonic Stem Cell Surfaces Reveals an Unusual Abundance of High-Mannose Glycans." <em>Molecular and Cellular Proteomics</em>, 11:M111.010660 (PMID 22147732). <a href="2012 Publications/Publication number 1.pdf" target= "_blank">View PDF</a>
<li> Jgamadze, J., J. Bergen, D. Stone, J-H. Jang, D.V. Schaffer, E.Y. Isacoff, and S. Pautot (2012) "Colloids as Mobile Substrates for the Implantation and Integration of Differentiated Neurons into the Mammalian Brain." <em>PLoS ONE</em>, 7:e30293 (PMID 22295079). <a href="2012 Publications/Publication number 2.pdf" target= "_blank">View PDF</a>
<li>Shah, P.S., N.P. Phan, and D.V. Schaffer (2012) "HIV Develops Indirect Cross-resistance to Combinatorial RNAi Targeting Two Distinct and Spatially Distant Sites." <em>Molecular Therapy</em>, 20:840-848 (PMID 22294151). <a href="2012 Publications/Publication number 3.pdf" target= "_blank">View PDF</a>
<li> Asokan, A., D.V. Schaffer, and R.J. Samulski (2012) "The AAV Vector Toolkit: Poised at the Clinical Crossroads." <em>Molecular Therapy</em>, 20:699-708 (PMID 22273577). <a href="2012 Publications/Publication number 4.pdf" target= "_blank">View PDF</a>
<li> Dey, S.S., Y. Xue, M.P. Joachimiak, G.D. Friedland, J.C. Burnett, Q. Zhou, A.P. Arkin, and D.V. Schaffer (2012) "Mutual Information Analysis Reveals Coevolving Residues in Tat that Compensate for Two Distinct Functions in HIV-1 Gene Expression." <em>Journal of Biological Chemistry</em>, 287:7945-7955 (PMID 22253435). <a href="2012 Publications/Dey_HIVTatMI_JBC_12.pdf" target= "_blank">View PDF</a>
<li> Bartel, M.A., J.R. Weinstein, and D.V. Schaffer (2012) "Directed Evolution of Novel Adeno-Associated Viruses for Therapeutic Gene Delivery." <em>Gene Therapy</em>, 19:694-700 (PMID 22402323). <a href="2012 Publications/Publication number 6.pdf" target= "_blank">View PDF</a>
<li> Miller-Jensen, K., S.S. Dey, N. Pham, J.E. Foley, A.P. Arkin, and D.V. Schaffer (2012) "Chromatin Accessibility at the HIV LTR Promoter Sets a Threshold for NF-κB Mediated Viral Gene Expression." <em>Integrative Biology</em>, 4:661-671 (PMID 22555315). <a href="2012 Publications/Publication number 7.pdf" target= "_blank">View PDF</a>
<li> Asuri, P., M.A. Bartel, T. Vazin, J-H. Jang, T.B. Wong, and D.V. Schaffer (2012) "Directed Evolution of Adeno-Associated Virus for Enhanced Gene Delivery and Gene Targeting in Human Pluripotent Stem Cells." <em>Molecular Therapy</em>, 20(2):329-38 (PMID 22108859). <a href="2012 Publications/Directed Evolution of Adeno-associated Virus for Enhanced Gene Delivery and Gene Targeting in Human Pluripotent Stem Cells.pdf" target= "_blank">View PDF</a>
</li><li> Perneta, V., S. Joly, D. Dalkara, O. Schwarz, Franziska Christ, D. Schaffer, J.G. Flannery, and M.E. Schwab "Neuronal NOGO-A Up-Regulation Does Not Contribute to ER Stress-Associated Apoptosis but Participates in the Regenerative Response in the Axotomized Adult Retina." <em>Cell Death and Degeneration</em>, 19:1096-1108 (PMID 22193546). <a href="2012 Publications/Neuronal Nogo-A upregulation does not contribute to ER stress-associated apoptosis but participates in the regenerative response in the axotomized adult retina.pdf" target= "_blank">View PDF</a>
</li>
</li>
</ul>
</div>
<h3>2011</h3>
<div>
<ul>
<li>Little, L., K. Dane, P.S. Daugherty, K.E. Healy, and D.V Schaffer (2011) "Exploiting Bacterial Peptide Display Technology to Engineer Biomaterials for Neural Stem Cell Culture." <em>Biomaterials</em>, 32:1484-1494 (PMID 21129772). <a href="2011 Publications/schaffer.pdf" target= "_blank">View PDF</a></li>
<li>Dickinson, B.C., J. Peltier, D. Stone, D.V. Schaffer (co-corresponding author), and C.J Chang (2011) "Nox2 Redox Signaling Maintains Essential Cell Populations in the Brain." <em>Nature Chemical Biology</em>, 7:106-112 (PMID 21186346). <a href="2011 Publications/nox2_schaffer.pdf" target= "_blank">View PDF</a>
</li>
<li>Arkin, A.P. and D.V. Schaffer (2011) "Network News: Innovations in 21st Century Systems Biology." <em>Cell</em>, 144:844-849 (PMID 21414475). <a href="2011 Publications/Arkin Schaffer.pdf" target= "_blank">View PDF</a></li>
<li>Jang, J-H., J.T. Koerber, J-S. Kim, P. Asurim, T. Vazin, M. Bartel, A. Keung, I. Kwon, K.I. Park, and D.V. Schaffer (2011) "An Evolved Adeno-Associated Viral Variant Enhances Gene Delivery and Gene Targeting in Neural Stem Cells." <em>Molecular Therapy</em>, 19:667-675 (PMID 21224831).<a href="2011 Publications/Jang J.pdf" target="_blank">View PDF</a></li>
<li>Yin, Y., K. Greenberg, J.J. Hunter, D. Dalkara, K.D. Kolstad, B.D. Masella, R. Wolfe, M. Visel, D. Stone, R.T. Libby, D. DiLoreto, Jr., D. Schaffer, J. Flannery, D.R. Williams, W.H. Merigan (2011)
"Intravitreal Injection of AAV2 Transduces Macaque Inner Retina." <em>Investigative Ophthalmology and Visual Science</em>, 52:2775-2783 (PMID 21310920). <a href="2011 Publications/Dalkara Kolstad.pdf" target="_blank">View PDF</a>
</li>
<li>Peltier, J., A. Conway, A. Keung, and D.V. Schaffer (2011) "Akt Increases Sox2 Expression in Adult Hippocampal Neural Progenitor Cells but Increased Sox2 does not Promote Proliferation." <em>Stem Cells and Development</em>, 20:1153-1161 (PMID 21028992).
</li>
<li>Chen, S., N. Patel, D.V. Schaffer, and M.M. Maharbiz (2011) "Trap and Corral: A Two-step Approach for Constructing and Constraining Dynamic Cell Contact Events in Differentiating Progenitor Cell Populations." <em>Journal of Micromechanics and Microengineering</em>,
5:054027. <a href="2012 Publications/Trap and Corral; A Two-Step Approach for Constructing and Constraining Dynamic Cell Contact Events in DIfferentiating Progenitor Cell Populations.pdf" target= "_blank">View PDF</a>
</li>
<li>Dalkara, D., K.D. Kolstad, K.I. Guerin, N.V. Hoffmann, M. Visel, R.R. Klimczak, D.V. Schaffer, and J.G. Flannery (2011) "AAV-mediated GDNF Secretion from Retinal Glia Slows Down Retinal Degeneration in a Rat Model of Retinitis Pigmentosa." <em>Molecular Therapy</em>, 19:1602-1608
(PMID 21522134). <a href="2012 Publications/AAV-Mediated GDNF Secretion from Retinal Glia Slows Down Retinal Degeneration in a Rat Model of Retinitis Pigmentosa.pdf" target= "_blank">View PDF</a>
</li>
<li>Ashton, R.A., A.J. Keung, J.L. Peltier, and D.V. Schaffer (2011) "Progress and Prospects for Stem Cell Engineering." <em>Annual Reviews in Chemical and Biomolecular Engineering</em>, 2:479-509. <a href="2012 Publications/Progress and Prospects from Stem Cell Engineering.pdf" target= "_blank">View PDF</a>
</li>
<li>Jang, J-H., D.V. Schaffer, and L. Shea (2011) "Engineering Biomaterial Systems to Enhance Viral Vector Gene Delivery." <em>Molecular Therapy</em>, 19:1407-1415 (PMID 21629221). <a href="2012 Publications/Engineering Biomaterial Systems to Enhance Viral Vector Gene Delivery.pdf" target= "_blank">View PDF</a>
</li>
<li>Miller-Jensen, K., S.S. Dey, D.V. Schaffer, and A.P. Arkin (2011) "Varying Virulence: Epigenetic Control of Expression Noise and Disease Processes." <em>Trends in Biotechnology</em>, 29:517-525 (PMID 21700350). <a href="2012 Publications/Varying Virulence- Epigenetic Control of Expression Noise and Disease Process.pdf" target= "_blank">View
PDF</a>
</li>
<li>Dickey, D., K. Excoffon, J. Koerber, J. Bergen, B. Steines, J. Klesney-Tait, D.V. Schaffer, and J. Zabner (2011) "Enhanced Sialic Acid-dependent Endocytosis Explains the Increased Airway Epithelia
Infection Efficiency of a Novel Adeno-associated Virus." <em>Journal of Virology</em>, 85:9023-9030 (PMID 21697483). <a href="2012 Publications/Enhanced Sialic Acid-Dependent Endocytosis Explains the Increased Airway Epithelia Infection Efficiency of a Novel Adeno-Associated Virus.pdf" target= "_blank">View PDF</a>
</li>
<li>Hester, M.E., M.J. Murtha, S. Song, M. Rao, C.J. Miranda, K. Meyer, J. Tian, G. Boulting, D.V. Schaffer, M.X. Zhu, S.L. Pfaff, F.H. Gage, and B.K. Kaspar (2011) "Rapid and Efficient Generation of
Functional Motor Neurons from Human Pluripotent Stem Cells Using Gene Delivered Transcription Factor Codes." <em>Molecular Therapy</em>, 19:1905-1912(PMID 21772256). <a href="2012 Publications/Rapid and Efficient Generation of Functional Motor Neurons from Human Pluripotent Stem Cells Using Gene Delivered Transcription Factor Codes.pdf" target= "_blank">View PDF</a>
</li>
<li>Shah, P.S. and D.V. Schaffer (2011) "Antiviral RNAi: Translating Science Toward Therapeutic Success." <em>Pharmaceutical Research</em>, 28:2966-2982 (PMID 21826573). <a href="2012 Publications/Antiviral RNAi- Translating Science Toward Therapeutic Success.pdf" target= "_blank">View PDF</a>
</li>
<li>Keung, A.J., E.M. de Juan-Pardo, D.V. Schaffer (co-corresponding author), and S. Kumar (2011) "Rho GTPases Mediate the Mechanosensitive Lineage Commitment of Neural Stem Cells." <em>Stem Cells</em>, 29: 1886-1897 (PMID 21956892). <a href="2012 Publications/Rho GTPases Mediate the Mechanosensitive Lineage Commitment of Neural Stem Cells.pdf" target= "_blank">View PDF</a></li>
<li>Bartel, M.A., D.V. Schaffer (co-corresponding author), and H. Buening (2011). "Enhancing the Clinical Potential of AAV Vectors by Capsid Engineering to Evade Pre-Existing Immunity." <em>Frontiers in Microbiology</em>, 2:204 (PMID 22065962). <a href="2012 Publications/Enhancing the Clinical Potential of AAV Vectors by Capsid Engineering to Evade Pre-Existing Immunity.pdf" target= "_blank">View PDF</a></li>
</ul>
</div>
<h3>2010</h3>
<div>
<ul>
<!--
<li>Yin, Y., K. Greenberg, J.J. Hunter, D. Dalkara, K.D. Kolstad, B.D. Masella, R. Wolfe, M. Visel, D. Stone, R.T. Libby, D. DiLoreto, Jr., D.V. Schaffer, J. Flannery, D.R. Williams, W.H. Merigan "Intravitreal Injection of AAV2 Transduces Macaque Inner Retina." Investigative Ophthalmology and Visual Science (in press). </li> -->
<li>Dickinson, B.C., J. Peltier, D. Stone, D.V. Schaffer (co-corresponding author), and C.J Chang "Nox2 Redox Signaling Maintains Essential Cell Populations in the Brain." <em>Nature Chemical Biology</em>, 7:106-112(PMID 21186346). <a href="2010 Publications/Dickinson NatureChemBio.pdf" target="_blank">View PDF</a></li>
<li>Little, L., K. Dane, P.S. Daugherty, K.E. Healy, and D.V Schaffer (2011) "Exploiting Bacterial Peptide Display Technology to Engineer Biomaterials for Neural Stem Cell Culture." <em>Biomaterials</em>, 32:1484-1494 (PMID 21129772). <a href="2010 Publications/little biomaterials.pdf" target= "_blank">View PDF</a></li>
<li>Saha, K., J. Kim, E. Irwin, J. Yoon, F. Momin, V. Trujillo, D.V. Schaffer, K.E. Healy, and R.C. Hayward "Surface Creasing Instability of Soft Polyacrylamide Cell Culture Substrates." <em>Biophysical Journal</em>, 99:L94-96 (PMID 21156124). <a href="2010 Publications/saha biophysical.pdf" target= "_blank">View PDF</a></li>
<li>Peltier, J., A. Conway, A. Keung, and D.V. Schaffer (2010) "Akt Increases Sox2 Expression in Adult Hippocampal Neural Progenitor Cells but Increased Sox2 does not Promote Proliferation." <em>Stem Cells and Development</em> (PMID 21028992). <a href="2012 Publications/.pdf" target= "_blank">View PDF</a></li>
<li>Willerth, S.M., H.A.M. Pedro, L. Pachter, L.M. Humeau, A.P. Arkin, and D.V. Schaffer (2010) "Development of a Low Bias Method for Characterizing Viral Populations Using Next Generation Sequencing Technology." <em>Public Library of Science (PLoS) ONE</em> 5:e13564 (PMID 21042592). <a href="2010 Publications/Willerth_HIVilluminaSeq_PLOSONE_10.pdf" target="_blank">View PDF</a></li>
<li>Skupsky, R., J.C. Burnett, J.E. Foley, D.V. Schaffer (co-corresponding author), and A.P. Arkin (2010) "HIV Promoter Integration Site Primarily Modulates Transcriptional Burst Size Rather Than Frequency." <em>Public Library of Science (PLoS) Computational Biology</em>, 6:e1000952 (PMID 20711350). <a href="2010 Publications/Skupsky_Bursting_PLoSCompBio_10.pdf" target="_blank">View PDF</a></li>
<li>Keung, A.J., S. Kumar, and D.V. Schaffer (2010) "Presentation Counts: Microenvironmental Regulation of Stem Cells by Biophysical and Material Cues." <em>Annual Reviews in Cell and Developmental Biology</em>, 26:533-556 (PMID 20590452). <a href="2010 Publications/Keung_NicheReview_ARCDB_10.pdf" target="_blank">View PDF</a></li>
<li>Ananthanarayanan, B., L. Little, D.V. Schaffer, K.E. Healy, and M. Tirrell (2010) "Neural Stem Cell Adhesion and Proliferation on Phospholipid Bilayers Functionalized with RGD Peptides." <em>Biomaterials</em>, 31:8706-15 (PMID 20728935).<a href="2010 Publications/Anathanarayanan Biomaterials.pdf" target="_blank">View PDF</a></li>
<li>Schaffer, D.V. (2010) "Microarraying for Mechanosensitivity." <em>Cell Stem Cell</em>, 7:273-4 (PMID 20804961). <a href="2010 Publications/Schaffer CellSC.pdf" target= "_blank">View PDF</a></li>
<li>Keung, A.J., K.E. Healy, S. Kumar, and D.V. Schaffer (2010) "Biophysics and Dynamics of Natural and Engineered Stem Cell Microenvironments." <em>Wiley Interdisciplinary Reviews (WIRES): Systems Biology and Medicine</em>, 2:49-64 (PMID 20836010). <a href="2010 Publications/Keung Wiley.pdf" target= "_blank">View PDF</a></li>
<li>Aviran, S., P.S. Shah, D.V. Schaffer, and A.P. Arkin (2010) "Computational Models of HIV-1 Resistance to Gene Therapy Elucidate Therapy Design Principles." <em>Public Library of Science (PLoS) Computational Biology</em>, 6:e1000883 (PMID 20711350). <a href="2010 Publications/Aviran_HIVmodel_PLoSCompBio_10.pdf" target="_blank">View PDF</a></li>
<li>Lim, K-I., R.R. Klimczak, J.H. Yu, and D.V. Schaffer (2010) "Specific Insertions of Zinc Finger Domains into Gag-Pol Yields Engineered Retroviral Vectors with Selective Integration Properties." <em>Proceedings of the National Academy of Sciences USA</em>, 107:12475-12480 (PMID 20616052). <a href="2010 Publications/Lim_ZFDretroviralIntegration_PNAS_10.pdf" target="_blank">View PDF</a></li>
<li>Jang, J-H., J.T. Koerber, K. Gujraty, S.R. Bethi, R.S. Kane, and D.V. Schaffer "Surface Immobilization of Hexa-histidine-tagged Adeno-associated Viral Vectors for Localized Gene Delivery." <em>Gene Therapy</em>, 17:1384-1389 (PMID 20508598). <a href="2010 Publications/Jang_AAVsurface_GT_10.pdf" target="_blank">View PDF</a></li>
<li>Shah, P.S. and D.V. Schaffer (2010) "Gene Therapy Takes a Cue from HAART: Combinatorial Antiviral Therapeutics Reach the Clinic." <em>Science Translational Medicine</em>, 2:36ps30 (PMID 20555021). <a href="2010 Publications/Shah_RossiCommentary_STM_10.pdf" target="_blank">View PDF</a></li>
<li>Burnett, J.C., K.I. Lim, A. Calafi, J.J. Rossi, D.V. Schaffer (co-corresponding author), A.P. Arkin (2010) "Combinatorial Latency Reactivation for HIV-1 Subtypes and Variants." <em>Journal of Virology</em>, 84:5958-5974 (PMID 20357084). <a href="2010 Publications/Burnett_CombinatorialAntiLatency_JV_2010.pdf" target="_blank">View PDF</a></li>
<li>Kurpinski, K., H. Lam, J. Chu, A. Kim, E. Tsay, S. Agrawal, D.V. Schaffer, A. Winoto, and S. Li (2010) "Transforming Growth Factor-beta and Notch Signaling Mediate Stem Cell Differentiation into Smooth Muscle Cells." <em>Stem Cells</em>, 4:734-42 (PMID 20146266).</li>
<li>Kolstad, K.D., D. Dalkara, K. Guerin, M. Visel, N. Hoffmann, D.V. Schaffer, and J.G. Flannery (2010) "Changes to AAV Mediated Gene Delivery in Retinal Degeneration." <em>Human Gene Therapy</em>, 5:571-8 (PMID 20021232).</li>
<li>Peltier, J. and D.V. Schaffer (2010) "Viral Packaging and Transduction of Adult Hippocampal Neural Progenitors." <em>Methods in Molecular Biology</em>, 621:103-116 (PMID 20405362). <a href="2010 Publications/Peltier_ANSCviralTransduction_MMB_10.pdf" target="_blank">View PDF</a></li>
<li>Robertson, M.J., J. Peltier, and D.V. Schaffer (2010) "In Vivo Analysis of Engrafted Adult Hippocampal Neural Progenitors." <em>Methods in Molecular Biology</em>, 621:89-102 (PMID 20405361). <a href="2010 Publications/Robertson_InVivoHippProgen_MMB_10.pdf" target="_blank">View PDF</a></li>
<li>Peltier, J., S. Agrawal, M.J. Robertson, and D.V. Schaffer (2010) "In Vitro Culture and Analysis of Adult Hippocampal Neural Progenitors." <em>Methods in Molecular Biology</em>, 621:65-87 (PMID 20405360). <a href="2010 Publications/Peltier_ANSCculture_MMB_10.pdf" target="_blank">View PDF</a></li>
<li>Peltier, J., B.K. Ormerod, and D.V. Schaffer (2010) "Isolation of Adult Hippocampal Neural Progenitors." <em>Methods in Molecular Biology</em>, 621:57-63 (PMID 20405359). <a href="2010 Publications/Peltier_ANSCisolation_MMB_10.pdf" target="_blank">View PDF</a></li>
<li>Vazin, T. and D.V. Schaffer (2010) "Engineering Strategies to Emulate the Stem Cell Niche Trends in Biotechnology." <em>Trends in Biotechnology</em>, 28:117-124 (PMID 20042248). Selected as the Cover Article. <a href="2010 Publications/Vazin_NicheReview_TibTech_10.pdf" target="_blank">View PDF</a></li>
<li>Peltier, J. and D.V. Schaffer (2010) "Systems Biology Approaches to Understanding Stem Cell Fate Choice."<em>IET Systems Biology</em>, 4:1-11 (PMID 20001088). <a href="2010 Publications/Peltier_StemCellsSysBioRev_IETSysBio_10.pdf" target="_blank">View PDF</a></li>
</ul>
</div>
<h3>2009</h3>
<div>
<ul>
<li>Meng, Y., S. Eshghi, Y. Li, R. Schmidt, D.V. Schaffer, and K.E. Healy (2009) "Characterization of Integrin Engagement during Defined Human Embryonic Stem Cell Culture." FASEB Journal, 24:0 (PMID 19933311). <a href="2009 Publications/Meng_hESCBiomat_FASEB_09.pdf" target="_blank">View PDF</a></li>
<li>J.T. Koerber, R. Klimczak, J-H. Jang, D. Dalkara, J.G. Flannery, and D.V. Schaffer (2009) "Molecular Evolution of Adeno-Associated Virus for Enhanced Glial Gene Delivery." Molecular Therapy, 17: 2088-2095 (PMID 19672246). <a href="2009 Publications/Koerber_AAVevolutionGlia_MT_09.pdf" target="_blank">View PDF</a></li>
<li>Carlson, M.E., M.J. Conboy, M. Hsu, L. Barchas, J. Jeong, A. Agrawal, A.J. Mikels, S. Agrawal, D.V. Schaffer, and I.M. Conboy (2009) "Relative Roles of TGF-beta1 and Wnt in the Systemic Regulation and Aging of Satellite Cell Responses." Aging Cell, 8:676-689 (PMID 19732043).</li>
<li>R. Klimczak, J.T. Koerber, D. Dalkara, J.G. Flannery, and D.V. Schaffer (2009) "A Novel Adeno-Associated Viral Variant for Efficient and Selective Intravitreal Transduction of Rat MÜller Cells." Public Library of Science (PLoS) ONE, 4:e7467 (PMID 19826483). <a href="2009 Publications/Klimczak_Shh10MullerCells_PLoSONE_09.pdf" target="_blank">View PDF</a></li>
<li>Dalkara, D., K.D. Kolstad, N. Caporale, M. Visel, R.R. Klimczak, D.V. Schaffer, and J.G. Flannery (2009) "Inner Limiting Membrane Barriers to AAV Mediated Retinal Transduction from the Vitreous." Molecular Therapy, 17:2096-2102 (PMID 19672248). <a href="2009 Publications/Dalkara_ILMbarrier_MT_09.pdf" target="_blank">View PDF</a></li>
<li>Banerjeea, A., M. Arhaa, S. Choudhary, R.S. Ashton, S.R. Bhatia, D.V. Schaffer, and R.S. Kane (2009) "The Influence of Hydrogel Modulus on the Proliferation and Differentiation of Encapsulated Neural Stem Cells." Biomaterials, 30:4695-9 (PMID 19539367). <a href="2009 Publications/Banerjee_NSCmodulusAlginate_Biomats_09.pdf" target="_blank">View PDF</a></li>
<li>Agrawal, S., C. Archer, and D.V. Schaffer "Computational Models of the Notch Network Elucidate Mechanisms of Context-dependent Signaling." Public Library of Science (PLoS) Computational Biology (in press). <a href="2008 Publications/Agrawal_NotchModel_PLoSCompBio_09.pdf" target="_blank">View PDF</a></li>
<li>Excoffon, K.J., J.T. Koerber, D.D. Dickey, M. Murtha, S. Keshavjee, B.K. Kaspar, J. Zabner, and D.V. Schaffer (2009) "Directed Evolution of Adeno-Associated Virus to an Infectious Respiratory Virus." Proceedings of the National Academy of Sciences USA, 106:3865-3870 (PMID 19237554). <a href="2008 Publications/Excoffon_AAVlung_PNAS_09.pdf" target="_blank">View PDF</a></li>
<li>Burnett, J.C., K. Miller-Jensen, P.S. Shah, A.P. Arkin, and D.V. Schaffer (2009) "Control of Stochastic Gene Expression by Host Factors at the HIV Promoter." Public Library of Science (PLoS) Pathogens, 5(1) (PMID 19132086). <a href="2008 Publications/Burnett_HIVstochFactors_PLoSPath_09.pdf" target="_blank">View PDF</a></li>
</ul>
</div>
<h3>2008</h3>
<div>
<ul>
<li>Leonard, J.N., P.S. Shah, J.C. Burnett, and D.V. Schaffer (2008) "HIV Evades RNA Interference Directed at TAR by an Indirect Compensatory Mechanism." Cell Host and Microbe, 4:484-94 (PMID 18996348). Selected as the Featured Article. <a href="2008 Publications/Leonard_HIVRNAi_CHM_08.pdf" target="_blank">View PDF</a></li>
<li>Saha, K., A.J. Keung, E.F. Irwin, Y. Li, D.V. Schaffer (co-corresponding author), and K.E. Healy "Substrate Modulus Directs Neural Stem Cell Behavior." Biophysical Journal, 95:4426-38. (PMID 18658232). <a href="2008 Publications/Saha Modulus NSC BioPhyJ'08.pdf" target="_blank">View PDF</a></li>
<li>Eshghi, S. and D.V. Schaffer "Engineering Microenvironments to Control Stem Cell Fate and Function." Stembook Stembook, D. Melton, ed. <a href="2008 Publications/Eshghi_EngMicroenvRev_Stembook_08.pdf" target="_blank">View PDF</a></li>
<li>Koerber, J.T., J-H. Jang, and D.V. Schaffer "DNA Shuffling of Adeno-Associated Virus Yields Functionally Diverse Viral Progeny." Molecular Therapy, 16:1703-9. (PMID 18728640). <a href="2008 Publications/Koerber_AAVshuffling_MT_08.pdf" target="_blank">View PDF</a></li>
<li>Koerber, J.T. and D.V. Schaffer "Transposon-Based Mutagenesis Generates Diverse Adeno-Associated Viral Libraries with Novel Gene Delivery Properties." Methods in Molecular Biology, 434:161-170 (PMID 18470644). <a href="2008 Publications/Transposon mutagenesis Gene Therapy Protocols.pdf" target="_blank">View PDF</a></li>
<li>Little, L., K.E. Healy, and D.V. Schaffer (2008) "Engineering Biomaterials for Synthetic Neural Stem Cell Microenvironments." Chemical Reviews, 108:1787-1796 (PMID 18476674). <a href="2008 Publications/Little_SynBiomatsNSCRev_ChemRev_08.pdf" target="_blank">View PDF</a></li>
<li>Wall, S., K. Saha, R. Aston, K. Kam, D.V. Schaffer, and K.E. Healy "Multivalency of Sonic Hedgehog Conjugated to Linear Polymer Chains Modulates Protein Potency." Bioconjugate Chemistry, 19:806-812 (PMID 18380472). <a href="2008 Publications/Wall_ShhMultivalency_BC_08.pdf" target="_blank">View PDF</a></li>
<li>Schaffer, D.V. (corresponding author), J.T. Koerber, and K.-I. Lim (2008) "Molecular Engineering of Viral Gene Delivery Vehicles." Annual Reviews in Biomedical Engineering, 10:169-94 (PMID 18647114). <a href="2008 Publications/Schaffer_GeneDeliveryReview_ARBE_08.pdf" target="_blank">View PDF</a></li>
<li>Lim, K-I., and D.V. Schaffer (2008) "Library Selection Approaches to Engineering Enhanced Retroviral and Lentiviral Vectors." Combinatorial Chemistry and High Throughput Screening, 11:111-117 (PMID 18336204). <a href="2008 Publications/Lim_RetroLentiLibrary_CCHTS_08.pdf" target="_blank">View PDF</a></li>
<li>Ricci, C., V. Pastukh, J. Leonard, J. Turrens, G. Wilson, D. Schaffer, and S.W. Schaffer(2008) "Mitochondrial DNA Damage Triggers Mitochondrial Superoxide Generation and Apoptosis." American Journal of Physiology, 294:C413-22 PMID 18077603).</li>
<li>Kwon, I. and D.V. Schaffer (2008) "Designer Gene Delivery Vectors: Molecular Engineering and Evolution of Adeno-Associated Viral Vectors for Enhanced Gene Transfer." Pharmaceutical Research, 25:489-499. <a href="2007 Publications/Kwon_AAVreview_PharmRes_07.pdf" target="_blank">View PDF</a></li>
<li>Robertson, M.J., P. Gip, and D.V. Schaffer (2008) "Neural Stem Cell Engineering: Directed Differentiation of Adult and Embryonic Stem Cells into Neurons." Frontiers in Bioscience, 13:21-50 (PMID 17981526). <a href="2008 Publications/Robertson_Prfs_FrontBiosci_08.pdf" target="_blank">View PDF</a></li>
</ul>
</div>
<h3>2007</h3>
<div>
<ul>
<li>Ashton, R.S., A. Banerjee, S. Punyani, D.V. Schaffer, and R.S Kane (2007) "Scaffolds Based on Degradable Alginate Hydrogels and Poly(lactide-co-glycolide) Microspheres for Stem Cell Culture Biomaterials." Biomaterials, 28:5518-5525. <a href="2007 Publications/Ashton_Alginate_Biomats_07.pdf" target="_blank">View PDF</a></li>
<li>Ashton, R.S., J. Peltier, C.A. Fasano, A. O'Neill, J. Leonard, S. Temple, D.V. Schaffer (co-corresponding author), and R.S. Kane (2007) "High-Throughput Screening of Gene Function in Stem Cells Using Clonal Microarrays." Stem Cells, 25:2928-2935. <a href="2007 Publications/Ashton_StemCellMicroarray_StemCells_07.pdf" target="_blank">View PDF</a></li>
<li>Jang, J-H., K-I. Lim, and D.V. Schaffer (2007) "Library Selection and Directed Evolution Approaches to Engineering Targeted Viral Vectors." Biotechnology & Bioengineering, 98:515-524. <a href="2007 Publications/Jang_DirEvoRev_B&B_07.pdf" target="_blank">View PDF</a></li>
<li>Saha, K., J. Pollock, D.V. Schaffer (co-corresponding author), and K.E. Healy (2007) "Designing Synthetic Materials to Control Stem Cell Phenotype." Current Opinion in Chemical Biology, 11:381-387. <a href="2007 Publications/Saha_SCBiomatRev_COCB_07.pdf" target="_blank">View PDF</a></li>
<li>Ho, J.E., E.H. Chung, S. Wall, D.V. Schaffer, and K.E. Healy (2007) "Immobilized Sonic Hedgehog N-Terminal Signaling Domain Enhances Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells." Journal of Biomedical Materials Research Part A, 83A:1200-1208. <a href="2007 Publications/Ho_ShhN_JBMR_07.pdf" target="_blank">View PDF</a></li>
<li>Peltier, J., A. O'Neill, and D.V. Schaffer (2007) "PI3K/Akt and CREB Regulate Adult Neural Hippocampal Progenitor Proliferation and Differentiation." Developmental Neurobiology, 67:1348-1361. <a href="2007 Publications/Peltier_NPCAkt_DevNeuro_07.pdf" target="_blank">View PDF</a></li>
<li>Koerber, J.T., J-H. Jang, and D.V. Schaffer (2007) "Engineering Adeno-Associated Virus for One Step Purification via Immobilized Metal Affinity Chromatography." Human Gene Therapy, 18:367-378. <a href="2007 Publications/Koerber_AAVhexahis_HGT_07s.pdf" target="_blank">View PDF</a></li>
<li>Leonard, J.N., P. Ferstl, A. Delgado, and D.V. Schaffer (2007) "Enhanced Preparation of Adeno-Associated Viral Vectors by Using High Hydrostatic Pressure to Selectively Inactivate Helper Adenovirus." Biotechnology & Bioengineering, 97:1170-1179. Selected as a Journal Spotlight Article. <a href="2007 Publications/Leonard_AAVpressure_BnB_07.pdf" target="_blank">View PDF</a></li>
<li>Saha, K., E.F. Irwin, J. Kozhukh, D.V. Schaffer (corresponding author), and K.E. Healy (corresponding author) (2007) "Biomimetic Interfacial Interpenetrating Polymer Networks Control Neural Stem Cell Behavior." Journal of Biomedical Materials Research Part A, 81:240-249. <a href="2007 Publications/Saha_NSCandIPN_JBMR_06.pdf" target="_blank">View PDF</a></li>
</ul>
</div>
<h3>2006</h3>
<div>
<ul>
<li>Greenberg, K.P., S.F. Geller, D.V. Schaffer, and J.G. Flannery (2006) "Targeted Transgene Expression in Muller Glia of Normal and Diseased Retinas Using Lentiviral Vectors." Investigative Ophthalmology & Visual Science, 48:1844-1852. <a href="2006 Publications/Greenberg_LentiMuller_IOVS_2007.pdf" target="_blank">View PDF</a></li>
<li>Yu, J.H. and D.V. Schaffer (2006) "High-Throughput, Library-Based Selection of a Murine Leukemia Virus to Infect Nondividing Cells." Journal of Virology, 80:8981-8988. Selected as a Journal of Virology Spotlight Article. <a href="2006 Publications/Yu_MLVGagPolNLS_JV_06.pdf" target="_blank">View PDF</a></li>
<li>Jang, J-H. and D.V. Schaffer (2006) "Microarraying the Cellular Microenvironment" Molecular Systems Biology, 2:39. <a href="2006 Publications/Jang_N&V_MSB_06.pdf" target="_blank">View PDF</a></li></li>
<li>Koerber, J.T., N. Maheshri, B.K. Kaspar, and D.V. Schaffer (2006) "Construction of Diverse Adeno-Associated Viral Libraries for Directed Evolution of Enhanced Gene Delivery Vehicles." Nature Protocols, 1:701-706. <a href="2006 Publications/Koerber_NatProtocols_06.pdf" target="_blank">View PDF</a></li>
<li>Greenberg K.P., E.S. Lee, D.V. Schaffer, and J.G. Flannery (2006) "Gene Delivery to the Retina Using Lentiviral Vectors." Advances in Experimental Medicine and Biology, 572: 255-266. <a href="2006 Publications/Greenberg_2006.pdf" target="_blank">View PDF</a></li>
<li>Yu, J. and D.V. Schaffer (2006) "Selection of Novel Vesicular Stomatitis Virus Glycoprotein Variants from a Peptide Insertion Library for Enhanced Purification of Retroviral and Lentiviral Vectors." Journal of Virology, 80:3285-3292. <a href="2006 Publications/Yu_VSVGrndmIns_JV_06.pdf" target="_blank">View PDF</a></li>
<li>Saha, K. and D.V. Schaffer (2006) "Signaling Dynamics in Sonic Hedgehog Tissue Patterning." Development, 133:889-900. <a href="2006 Publications/Saha_ShhDyn_Dev_06.pdf" target="_blank">View PDF</a></li>
<li>Maheshri, N., J.T. Koerber, B. Kaspar, and D.V. Schaffer (2006) "Directed Evolution of Adeno-Associated Virus Yields Enhanced Gene Delivery Vectors." Nature Biotechnology, 24:198-204. <a href="2006 Publications/Maheshri_AAVevo_NBT_06.pdf" target="_blank">View PDF</a></li>
<li>Abranches, E., O'Neill, A., Robertson, M.J., Schaffer, D.V. (corresponding author), and J. Cabral (2006) "Development of Quantitative PCR Methods to Analyze Adult Neural Progenitor Cell Culture State." Biotechnology & Applied Biochemistry, 44:1-8. <a href="2006 Publications/Elsa_QRTPCRnpcs_BAB_06.pdf" target="_blank">View PDF</a></li>
<li>Leonard, J. and D.V. Schaffer (2006) "Antiviral RNAi Strategies: Emerging Approaches for Hitting a Moving Target." Gene Therapy, 13:532-540. <a href="2006 Publications/Leonard_AntiviralRNAiRev_GT_05.pdf" target="_blank">View PDF</a></li>
</ul>
</div>
<h3>2005</h3>
<div>
<ul>
<li>Weinberger, L.S., J.C. Burnett, J.E. Toettcher, A.P. Arkin, and D.V. Schaffer (2005) "Stochastic Gene Expression in a Lentiviral Feedback Loop: HIV-1 Tat Expression Drives Phenotypic Diversity." Cell, 122:169-182. <a href="2005 Publications/Weinberger_Cell_05.pdf" target="_blank">View PDF</a></li>
<li>Yu, J. and D.V. Schaffer (2005) "Advanced Targeting Strategies for Murine Retroviral and Adeno-Associated Viral Vectors." Advances in Biochemical Engineering & Biotechnology, 99:147-167. <a href="2005 Publications/Yu_05.pdf" target="_blank">View PDF</a></li></li>
<li>Schaffer, D.V. and W.C. Zhou (2005) "Gene Therapy and Gene Delivery Systems as Future Human Therapeutics." Advances in Biochemical Engineering & Biotechnology, 99:1-5.</li>
<li>Lee, G., Maheshri, N., Kaspar, B.K., and D.V. Schaffer (2005) "PEG Conjugation Moderately Protects Adeno-Associated Viral Vectors Against Antibody Neutralization." Biotechnology & Bioengineering, 92:24-34. <a href="2005 Publications/Lee_AAVPEG_BB_05.pdf" target="_blank">View PDF</a></li>
<li>Agrawal, S. and D.V. Schaffer (2005) "In Situ Stem Cell Therapy: Novel Targets, Familiar Challenges." Trends in Biotechnology, 23:78-83. <a href="2005 Publications/Agrawal_InSituSCT_TiBtech_05.pdf" target="_blank">View PDF</a></li>
<li>Leonard, J. and D.V. Schaffer (2005) "Computational Design of RNA Interference Strategies that Resist HIV Escape." Journal of Virology, 79:1645-1654. <a href="2005 Publications/Leonard_CompRNAiHIV_JV_05.pdf" target="_blank">View PDF</a></li>
</ul>
</div>
<h3>2004</h3>
<div>
<ul>
<li>O'Neill, A. and D.V. Schaffer (2004) "The Biology and Engineering of Stem Cell Control." Biotechnology & Applied Biochemistry, 40:5-16. <a href="2004 Publications/ONeill_SCRev_BAB_04.pdf" target="_blank">View PDF</a></li>
<li>Lai, K., M.J. Robertson, and D.V. Schaffer (2004) "The Sonic Hedgehog Signaling System as a Bistable Genetic Switch." Biophysical Journal, 86:2748-2757. <a href="2004 Publications/Lai_ShhNet_BJ_04.pdf" target="_blank">View PDF</a></li>
<li>Ignowski, J. and D.V. Schaffer (2004) "Kinetic Analysis and Modeling of Firefly Luciferase as a Quantitative Reporter Gene in Live Mammalian Cells." Biotechnology & Bioengineering, 86(7):827-34. <a href="2004 Publications/Ignowski_Luc_BB_04.pdf" target="_blank">View PDF</a></li>
<li>Schaffer, D.V. and F.H. Gage (2004) "Neurogenesis and Neuroadaptation" Neuromolecular Medicine, 5:1-9. <a href="2004 Publications/Schaffer_NNRev_NMM_04.pdf" target="_blank">View PDF</a></li>
</ul>
</div>
<h3>2003</h3>
<div>
<ul>
<li>Weinberger, L., D.V. Schaffer (corresponding author), and A.P. Arkin (corresponding author) (2003) "Theoretical Design of a Gene Therapy to Prevent AIDS but Not HIV-1 Infection." Journal of Virology, 77:10028-10036. <a href="2003 Publications/Weinberger_crHIVModel_JV_03.pdf" target="_blank">View PDF</a></li>
<li>Maheshri, N. and D.V. Schaffer (2003) "Computational and Experimental Analysis of DNA Shuffling." Proceedings of the National Academy of Sciences USA, 100:3071-3076. <a href="2003 Publications/Maheshri_PNAS_03.pdf" target="_blank">View PDF</a></li>
<li>Lai, K, B.K. Kaspar, F.H. Gage, and D.V. Schaffer (2003) "Sonic Hedgehog Regulates Adult Neural Progenitor Proliferation in Vitro and in Vivo." Nature Neuroscience, 6(1):21-27. <a href="2003 Publications/Lai_ShhNeurogen_NN_03.pdf" target="_blank">View PDF</a></li>
</ul></div>
<h3>2002</h3>
<div>
<ul>
<li>Wiewrodt, W., A.P. Thomas, L. Cipelletti, D.A. Weitz, S.I. Feinstein, D.V. Schaffer, S.M. Albelda, M. Koval, and V.R. Muzykantov (2002) "Size-Dependent Intracellular Immunotargeting into Endothelial Cells via a Surface Adhesion Molecule, PECAM-1," Blood, 99:912-22. <a href="2002 Publications/Wiewrodt_Blood_02.pdf" target="_blank">View PDF</a></li>
<li>Kaspar, B.K., Vissel, B., Bengoechea, T., Crone, S., Randolph-Moore, L., Muller, R., Brandon,E.P., Schaffer, D., Verma, I.M., Lee, K.F., Heinemann, S.F., and F.H. Gage (2002) "Adeno-associated Virus Effectively Mediates Conditional Gene Modification in the Brain." Proceedings of the National Academy of Sciences USA, 99:2320-5. <a href="2002 Publics ations/Kaspar_AAVCre_PNAS_06.pdf" target="_blank">View PDF</a></li>
<li>Kaspar, B.K., D. Erickson, D. Schaffer, L. Hinh, F.H. Gage, and D.A. Peterson (2002) "Targeted Retrograde Gene Delivery for Neuronal Protection." Molecular Therapy, 5:50-6. <a href="2002 Publications/Kaspar_MolTher_02.pdf" target="_blank">View PDF</a></li>
</ul></div>
<h3>2001</h3>
<div><ul><li>Mozaffari, M.S. and D.V. Schaffer (2001) "Taurine Modulates Arginine Vasopressin-Mediated Regulation of Renal Function." Journal of Cardiovascular Pharmacology, 37(6): 742-750.</li></ul></div>
<h3>2000</h3>
<div><ul><li>Schaffer, D.V. and D.A. Lauffenburger (2000) "Targeted Synthetic Gene Delivery Vectors." Current Opinions in Molecular Therapeutics, 2: 155-161.<a href="Early Publications/Schaffer_PolyplexRev_COMT_00.pdf" target="_blank">View PDF</a></li></ul></div>
<h3>1999</h3>
<div>
<ul>
<li>Schaffer, D.V., N. Fidelman, N. Dan, and D.A. Lauffenburger (1999) "Vector Unpackaging as a Potential Barrier for Receptor-Mediated Polyplex Gene Delivery." Biotechnology & Bioengineering, 67:598-606. <a href="Early Publications/Schaffer_VectorUnpack_B&B_99.pdf" target="_blank">View PDF</a></li>
<li>Lauffenburger, D.A. and D.V. Schaffer (1999) "The Matrix Delivers." Nature Medicine, 5: 733-734. <a href="Early Publications/Schaffer_NM_99.pdf" target="_blank">View PDF</a></li>
</ul></div>
<h3>1998</h3>
<div><ul><li>Schaffer, D.V. and D.A. Lauffenburger (1998) "Optimization of Cell Surface Binding Enhances Efficiency and Specificity of Molecular Conjugate Gene Delivery." Journal of Biological Chemistry, 273:28004-28009.<a href="Early Publications/Schaffer_JBC_1998.pdf" target="_blank">View PDF</a></li></ul></div>
<h3>1996</h3>
<div><ul><li>Schaffer, D.V., R.L. Neve, and D.A. Lauffenburger (1996) "Use of Green Fluorescent Protein as a Reporter of Epidermal Growth Factor Receptor-Mediated Gene Delivery." Tissue Engineering, 3:53-63.</li></ul></div>
<h3>1994</h3>
<div><ul><li>Gramer, M.J., D.V. Schaffer, M.B. Sliwkowski, and C.F. Goochee (1994) "Purification and Purification and Characterization of an a-L-Fucosidase from Chinese Hamster Ovary Cell Culture Supernatant." Glycobiology, 4:611-616.</li></ul></div>
</div>
</div> <!-- /container for publications -->
<div class="container webpage" id="meetings">
<div class="page-header">
<h1>Meetings and Events</h1>
</div>
<p>
<strong>Group meetings are held on Mondays in 521 Stanley Hall from 1:30-3pm</strong>
</p>
<p>
<strong>Spring 2014 Presenters:</strong>
<ul>
<li>1/13: Anusuya</li>
<li>1/27: Jayodita</li>
<li>2/3: Katerina</li>
<li>2/10: Mikhail's undergrad (?)</li>
<li>2/24: Handie</li>
<li>3/3: Brian</li>
<li>3/10: Tim</li>
<li>3/24: Alyssa</li>
<li>4/14: Yunsuk</li>
<li>4/28: Lukasz</li>
<li>5/12: Leah</li>
<li>5/19: Sean</li>
</ul>
<strong>Summer 2014 Presenters:</strong>
<ul>
<li>6/2: Prajit</li>
<li>6/16: Maroof</li>
<li>7/14: Jorge</li>
<li>7/28: Sebastian</li>
<li>8/11: Dawn</li>
</ul>
<strong>Fall 2014 Presenters:</strong>
<ul>
<li>9/8: Dawn</li>
<li>9/22: Barbara</li>
<li>9/29: Mervi</li>
<li>10/13: Jonathan Jui</li>
<li>10/20: Benjamin</li>
<li>11/3: Tandis</li>
<li>11/10: David O</li>
<li>11/17: Thomas</li>
<li>11/24: Mike</li>
<li>12/1: Sabrina</li>
<li>12/8: Riya</li>
<li>12/15: Dawn</li>
</ul>
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<strong>Note: Research Format = Everyone will be presenting their current research</strong>
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