velveth: Word length 33 greater than max allowed value (31).

[vasudhajain29]

I am trying to run velveth on my paired files but facing the following error:

[0.000000] Velvet can't handle k-mers as long as 33! We'll stick to 31 if you don't mind.
[0.024794] Reading FastA file /Users/Admin1/Desktop/ICGEB/Amplicon_Sequencing_18Aug/Fasta/Seq1.fasta;
[4.061945] 1282742 sequences found
[4.061957] Done
[4.062048] Reading FastA file /Users/Admin1/Desktop/ICGEB/Amplicon_Sequencing_18Aug/Fasta/Seq1_R2.fasta;
[8.000691] 1282742 sequences found
[8.000702] Done
[8.017212] Reading read set file out-dir/Sequences;
[8.848178] 2565484 sequences found
[11.843912] Done
[11.843930] 2565484 sequences in total.
[11.853344] Writing into roadmap file out-dir/Roadmaps...
[16.801721] Inputting sequences...
[16.801744] Inputting sequence 0 / 2565484
[30.299725] Inputting sequence 1000000 / 2565484
[44.107881] Inputting sequence 2000000 / 2565484
[52.422222] === Sequences loaded in 35.620505 s
[52.422345] Done inputting sequences
[52.422354] Destroying splay table
[52.452908] Splay table destroyed
velveth: Word length 33 greater than max allowed value (31).
Recompile Velvet to deal with this word length.: No such file or directory.

I have tried using
make 'MAXKMERLENGTH=92'

but still facing the above error.
Please help.

[dzerbino]

Hello @vasudhajain29 ,

When you say that you ran make again, was it successful? E.g. do you see a new file appear with ls?

Can you confirm that you are now using the newly compiled executable (e.g. using which velveth)?

Regards,

Daniel