diff --git a/index.js b/index.js
index 367bdba..9172c65 100644
--- a/index.js
+++ b/index.js
@@ -66,6 +66,10 @@ function csurf (options) {
   var ignoreMethod = getIgnoredMethods(ignoreMethods)
 
   return function csrf (req, res, next) {
+    if (verifyCsurfIsInitializedRepeatedly(req, cookie)) {
+      return next(new Error('csurf({cookie: true}) or csurf({cookie: {}}}) is repeatedly called with same middleware in the cooke mode, first validation will result in the invalid token'))
+    }
+
     // validate the configuration against request
     if (!verifyConfiguration(req, sessionKey, cookie)) {
       return next(new Error('misconfigured csrf'))
@@ -295,3 +299,24 @@ function verifyConfiguration (req, sessionKey, cookie) {
 
   return true
 }
+
+/**
+ * Check csurf initialization status.
+ * This function is only used in cookie mode.
+ * @param {IncomingMessage} req
+ * @param {Object} cookie
+ * @returns {boolean} csurf is initializaed repeatedly or not in cookie mode.
+ * @api private
+ */
+
+function verifyCsurfIsInitializedRepeatedly (req, cookie) {
+  if (!cookie) {
+    return false
+  }
+  var _name = '@@isCsurfInitialized@@'
+  if (!req[_name]) {
+    req[_name] = true
+    return false
+  }
+  return true
+}
diff --git a/test/test.js b/test/test.js
index 2e5d7b8..a27cda0 100644
--- a/test/test.js
+++ b/test/test.js
@@ -232,6 +232,20 @@ describe('csurf', function () {
               .expect(200, done)
           })
       })
+
+      it('should return error when call csurf twice in middleware.', function (done) {
+        var app = connect()
+        app.use(cookieParser('keyboard cat'))
+        app.use(csurf({ cookie: true }))
+        app.use(csurf({ cookie: true }))
+        app.use(function (req, res) {
+          res.end(req.csrfToken() || 'none')
+        })
+
+        request(app)
+          .get('/')
+          .expect(500, /csurf\({cookie: true}\) or csurf\({cookie: {}}}\) is repeatedly called with same middleware in the cooke mode, first validation will result in the invalid token/, done)
+      })
     })
 
     describe('when an object', function () {