NTR: trans-2,3-dihydro-3-hydroxyanthranilic acid synthase activity

[cmungall]

Please provide as much information as you can:

• Suggested term label:

trans-2,3-dihydro-3-hydroxyanthranilic acid synthase activity

• Definition (free text)

Catalysis of the reaction: (2S)-2-amino-4-deoxychorismate + H2O = (5S,6S)-6-amino-5-hydroxycyclohexa-1,3-diene-1-carboxyate + pyruvate

• Reference, in format PMID:#######

EC:3.3.2.15

• Gene product name and ID to be annotated to this term

Pseudomonas aeruginosa PhzD

Note that we will get the annotations to the 3 uniprot relevant uniprot IDs for free, because the RHEA annotations are correct (thanks RHEA!!)

• Parent term(s)

id: GO:0016803
name: ether hydrolase activity

consider also

part_of: phenazine biosynthetic process

• Children terms (if applicable) Should any existing terms that should be moved underneath this new proposed term?

None

• Synonyms (please specify, EXACT, BROAD, NARROW or RELATED)

• Cross-references

• For enzymes, please provide RHEA and/or EC numbers.

• Can also provide MetaCyc, KEGG, Wikipedia, and other links.

exact: EC:3.3.2.15
exact: RHEA:49456
exact: MetaCyc:RXN-9030

• Any other information

This enzyme is critical in the phenazine biosynthesis pathway of Pseudomonas aeruginosa, where it acts after the formation of 2-amino-4-deoxychorismate by PhzE, contributing to the production of antimicrobial phenazine compounds such as pyocyanin.

This pathway is very well characterized by KEGG and MetaCyc

Note that if you look at the current GO annotations to the D member of the operon, you will see they are to 3.3.2.1 / GO:0008908 / RHEA:11112 / isochorismatase activity, coming from IPR. While this is not strictly wrong (you can see the publications and the kinetics on the uniprot page), it's sort of misleading as this activity is more characteristic of the siderophin pathway in enterobacteria. We may want to follow up with IPR2GO mappings after this.