diff --git a/grafana-plugin/e2e-tests/.env.example b/grafana-plugin/e2e-tests/.env.example
deleted file mode 100644
index 9d3195e7b..000000000
--- a/grafana-plugin/e2e-tests/.env.example
+++ /dev/null
@@ -1,7 +0,0 @@
-GRAFANA_VIEWER_USERNAME=viewer
-GRAFANA_VIEWER_PASSWORD=viewer
-GRAFANA_EDITOR_USERNAME=editor
-GRAFANA_EDITOR_PASSWORD=editor
-GRAFANA_ADMIN_USERNAME=oncall
-GRAFANA_ADMIN_PASSWORD=oncall
-IS_OPEN_SOURCE=True
diff --git a/grafana-plugin/package.json b/grafana-plugin/package.json
index 07e4c1140..58c9fa07b 100644
--- a/grafana-plugin/package.json
+++ b/grafana-plugin/package.json
@@ -85,7 +85,6 @@
     "copy-webpack-plugin": "^11.0.0",
     "css-loader": "^6.7.3",
     "dompurify": "^2.3.12",
-    "dotenv": "^16.4.0",
     "eslint": "^8.25.0",
     "eslint-plugin-deprecation": "^2.0.0",
     "eslint-plugin-jsdoc": "^44.2.4",
diff --git a/grafana-plugin/playwright.config.ts b/grafana-plugin/playwright.config.ts
index a0aa9d6bb..c345c682d 100644
--- a/grafana-plugin/playwright.config.ts
+++ b/grafana-plugin/playwright.config.ts
@@ -1,11 +1,6 @@
 import { PlaywrightTestProject, defineConfig, devices } from '@playwright/test';
 
 import path from 'path';
-/**
- * Read environment variables from file.
- * https://github.com/motdotla/dotenv
- */
-require('dotenv').config({ path: path.resolve(process.cwd(), 'e2e-tests/.env') });
 
 export const VIEWER_USER_STORAGE_STATE = path.join(process.cwd(), 'e2e-tests/.auth/viewer.json');
 export const EDITOR_USER_STORAGE_STATE = path.join(process.cwd(), 'e2e-tests/.auth/editor.json');
diff --git a/grafana-plugin/pnpm-lock.yaml b/grafana-plugin/pnpm-lock.yaml
index 1264b5925..acb438ea5 100644
--- a/grafana-plugin/pnpm-lock.yaml
+++ b/grafana-plugin/pnpm-lock.yaml
@@ -253,9 +253,6 @@ importers:
       dompurify:
         specifier: ^2.3.12
         version: 2.5.6
-      dotenv:
-        specifier: ^16.4.0
-        version: 16.4.5
       eslint:
         specifier: ^8.25.0
         version: 8.57.0
@@ -2718,10 +2715,6 @@ packages:
   dot-case@3.0.4:
     resolution: {integrity: sha512-Kv5nKlh6yRrdrGvxeJ2e5y2eRUpkUosIW4A2AS38zwSz27zu7ufDwQPi5Jhs3XAlGNetl3bmnGhQsMtkKJnj3w==}
 
-  dotenv@16.4.5:
-    resolution: {integrity: sha512-ZmdL2rui+eB2YwhsWzjInR8LldtZHGDoQ1ugH85ppHKwpUHL7j7rN0Ti9NCnGiQbhaZ11FpR+7ao1dNsmduNUg==}
-    engines: {node: '>=12'}
-
   downshift@9.0.8:
     resolution: {integrity: sha512-59BWD7+hSUQIM1DeNPLirNNnZIO9qMdIK5GQ/Uo8q34gT4B78RBlb9dhzgnh0HfQTJj4T/JKYD8KoLAlMWnTsA==}
     peerDependencies:
@@ -9324,8 +9317,6 @@ snapshots:
       no-case: 3.0.4
       tslib: 2.5.3
 
-  dotenv@16.4.5: {}
-
   downshift@9.0.8(react@18.2.0):
     dependencies:
       '@babel/runtime': 7.25.6