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describe some common gotchas in the README #1

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grahamgower opened this issue Feb 11, 2022 · 0 comments
Open

describe some common gotchas in the README #1

grahamgower opened this issue Feb 11, 2022 · 0 comments

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@grahamgower
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Various problems can cause sex determination to be inconclusive (intermediate read dosage):

  • check library-level bams, to exclude bioinfo issues with accidentally merging libraries from distinct individuals
  • filter reads with low MAPQ (e.g. < 30)
  • ensure mito reads are excluded - some samples can have high MT/nuc ratio and if there is no mito sequence in the reference, the mito reads can map to NUMTs instead
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