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In the current implementation input BAM files are merged to the alignment files produced by the pipeline mapping step and then used for downstream processing. This approach can pose problems because:
merging external BAMs is risky as there is no guarantee that the data has been processed in a consistent way
sometime project data is kept for long-term storage in BAM format but we want fully reprocess the data with grape-nf (e.g. re-mapping with a different genome/annotation)
A possible better approach could be based on params.steps and act accordingly:
if the mapping step is specified the BAM files should be re-processed
if only downstream steps (e.g. quantification) are specified the BAM files are used as-is. An error should be reported If BAM files can be merged
Alternatives might be considered as well...
The text was updated successfully, but these errors were encountered:
In the current implementation input BAM files are merged to the alignment files produced by the pipeline
mapping
step and then used for downstream processing. This approach can pose problems because:grape-nf
(e.g. re-mapping with a different genome/annotation)A possible better approach could be based on
params.steps
and act accordingly:mapping
step is specified the BAM files should be re-processedquantification
) are specified the BAM files are used as-is. An error should be reported If BAM files can be mergedAlternatives might be considered as well...
The text was updated successfully, but these errors were encountered: