title | category |
---|---|
Single Cell Quality control with saturation |
Single Cell |
People often ask how many cells they need to sequence in their next experiment. Saturation analysis helps to answer that question looking at the current experiment. Would adding more coverage to the current experiment result in getting more transcripts, genes, or in just more duplicated reads?
First, use from bcbio to single cell script to load data from bcbio into SingleCellExperiment object.
Second, use this Rmd template to create report.