gene-therapy.mdwn

Here are some gene therapy delivery methods.

naked DNA

hydrodynamic injection

viruses and viral gene therapy

• CAV-2 - why a canine virus is a neurobiologist's best friend

electroporation

gene gun

sonoporation

gas-filled micro-bubbles

magnetofection and magnetic nanoparticles

• magnetic calcium phosphate nano-formulations

carbon nanotubes

quantum dots

• super paramagnetic iron oxide nanoparticles (SPIONS)

lipoplexes

dendrimers

inorganic nanoparticles

• carbon nanotuubes
• magnetic nanoparticles (see also magnetofection)
• calcium phosphate nanoparticles
• gold nanoparticles
• quantum dots
• C60 fullerenes
• aminofullerenes
• silica nanoparticles

gold nanoparticles

cell-penetrating peptides

polymerosomes

liposomes

polyplexes (DNA + plastic)

• polyion complex micelles (PICs)

cationic polymers

• collagen
• albumin
• beta-casein
• zein

cationic lipids

• dioleylpropyl trimethylammonium chloride (DOTMA)
• dioleoyl trimethylammonium propane (DOTAP)

cationic emulsions

solid lipid nanoparticles

virosomes

protofection

natural competence

Ligandal's nano-gobstopper method

(patent) Nanoparticle-mediated gene delivery, genomic editing and ligand-targeted modification in various cell populations

conjugation of PEI for DNA binding, histone tail peptide for nuclear localization, poly-glutamic acid, glutamic acid for stabilization and charge balancing or whatever, then you stick DNA to it, and silica coat the whole thing, then add even more cell-targeting stuff to the outside.

other

• poly lactic-co-glycolic acid (PLGA)-based nanoparticles
• hybrid nanoparticles such as liposome-polycation-DNA (LPD) nanoparticles
• lipopolyplexes

hplusroadmap logs

See http://gnusha.org/logs/2017-01-09.log for some preliminary stuff.