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all_counts.txt prints numbers instead of miRNA names #4

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tolarnik opened this issue Aug 1, 2022 · 0 comments
Open

all_counts.txt prints numbers instead of miRNA names #4

tolarnik opened this issue Aug 1, 2022 · 0 comments

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@tolarnik
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tolarnik commented Aug 1, 2022

Hello,

I am using the docker4seq through the GUI to perform miRNA counting on fastq file (using mus musculus - mmu - as a reference miRBase organism). All seems to be working well, there are no errors in the log file, yet in the all_counts.txt file, there are numbers instead of miRNA names -> see attached file.

I tried the downloadContainers again and all seems to be downloaded. Any idea why this might be happening? Alternatively, is there any way to somehow cross-ling the numbers in the all_couts.txt file with a list of miRNAs?

Thank you very much
all.counts.txt

output in Routput:


R version 3.6.3 (2020-02-29) -- "Holding the Windsock"
Copyright (C) 2020 The R Foundation for Statistical Computing
Platform: x86_64-pc-linux-gnu (64-bit)

R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.

  Natural language support but running in an English locale

R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.

Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.

> ##First read in the arguments listed at the command line
> args=(commandArgs(TRUE))
> 
> ##args is now a list of character vectors
> ## First check to see if arguments are passed.
> ## Then cycle through each element of the list and evaluate the expressions.
> if(length(args)==0){
+     print("No arguments supplied.")
+     ##supply default values
+ }else{
+     for(i in 1:length(args)){
+       eval(parse(text=args[[i]]))
+     }
+ }
> 
> 
> library(docker4seq)
> mirnaCounts(group = group, fastq.folder =  fastq.folder, scratch.folder =  scratch.folder, mirbase.id= mirbase.id, download.status=  download.status, adapter.type= adapter.type, trimmed.fastq= trimmed.fastq)

 In your system the following version of Docker is installed:
Docker version 20.10.17, build 100c70173a30db2e3381ac0eaf2e5b67b68ffe721d68ffa8dbaec24be9d2269ef386f8c

Docker ID is:
 73a30db2e338 
..


Docker exit status: 0 

FastQC analysis is finished
het2.fastq.gz
Started analysis of het2.fastq.gz_tmp
Approx 10% complete for het2.fastq.gz_tmp
Approx 20% complete for het2.fastq.gz_tmp
Approx 30% complete for het2.fastq.gz_tmp
Approx 35% complete for het2.fastq.gz_tmp
Approx 45% complete for het2.fastq.gz_tmp
Approx 60% complete for het2.fastq.gz_tmp
Approx 70% complete for het2.fastq.gz_tmp
Approx 80% complete for het2.fastq.gz_tmp
Approx 90% complete for het2.fastq.gz_tmp
Approx 100% complete for het2.fastq.gz_tmp
Analysis complete for het2.fastq.gz_tmp
73a30db2e3381ac0eaf2e5b67b68ffe721d68ffa8dbaec24be9d2269ef386f8c


Removing the temporary file ....

 In your system the following version of Docker is installed:
Docker version 20.10.17, build 100c701
creating a folder in scratch folder

setting as working dir the scratch folder and running mirna8 docker container
e9ec05bbfecce9ca26757b6050c8382f4287dfd610665fed15b46e9b712406b1

Docker ID is:
 e9ec05bbfecc 
...................................


Docker exit status: 0 

mirnaCounts analysis is finished
sh: 1: Syntax error: Bad fd number
e9ec05bbfecce9ca26757b6050c8382f4287dfd610665fed15b46e9b712406b1


Removing the rsemStar temporary file ....
> 
> 
> 
> proc.time()
   user  system elapsed 
  3.848   1.377 355.218 ```
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