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I have some peptides from a proteomics experiment (~5000) and I have a list of protein references that they may align to, sometimes not perfectly. I've used ncbi-blast SAM output option and a bit of datamunging to create a SAM/BAM file output of this with the use of samtools. The contents of the BAM file look good, but when I display them in samtools tview, the aminoacids in each SAM record are converted into A,C,G,T, plus ambiguity codes.
I then remembered jvarkit had a bam ttview module that produced a similar, in some aspects more rich output, than samtools tview, with some more flexibility.
Expected behaviour
Should I expect this to work? E.g. same basic behaviour as with DNA alphabet reference and NGS input, but with a protein alphabet and peptide input.
If this is beyond what jvarkit would be interested in supporting, that's perfectly understandable. There may be a non-SAM based approach to do that which is a better alternative than shoehorning proteins into the SAM/BAM toolkits out there.
Thanks in advance,
The text was updated successfully, but these errors were encountered:
Subject of the issue
I have some peptides from a proteomics experiment (~5000) and I have a list of protein references that they may align to, sometimes not perfectly. I've used ncbi-blast SAM output option and a bit of datamunging to create a SAM/BAM file output of this with the use of samtools. The contents of the BAM file look good, but when I display them in samtools tview, the aminoacids in each SAM record are converted into A,C,G,T, plus ambiguity codes.
I then remembered jvarkit had a bam ttview module that produced a similar, in some aspects more rich output, than samtools tview, with some more flexibility.
Expected behaviour
Should I expect this to work? E.g. same basic behaviour as with DNA alphabet reference and NGS input, but with a protein alphabet and peptide input.
If this is beyond what jvarkit would be interested in supporting, that's perfectly understandable. There may be a non-SAM based approach to do that which is a better alternative than shoehorning proteins into the SAM/BAM toolkits out there.
Thanks in advance,
The text was updated successfully, but these errors were encountered: