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GemMappabilityToBed has problems with some invalid characters in mappability files #531

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robmaz opened this issue Oct 19, 2018 · 1 comment
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Priority: Medium Status: Pending In discussion to include in the project backlog Type: Question User/Developer question to be answer

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@robmaz
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robmaz commented Oct 19, 2018

GemMappabilityToBed produces user errors with files generated by gem-mappability. I am attaching the files in question and the output of gem-mappability and readtools.

map2bed.out.gz
mappability_files.tar.gz
mappability.out.gz

@magicDGS magicDGS added Priority: Medium Type: Question User/Developer question to be answer Status: Pending In discussion to include in the project backlog labels Oct 21, 2018
@magicDGS
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@robmaz - thanks a lot for using an experimental tool and reporting an error, it is much appreaciated!

First, I should recognized that I found this before in one of my analyses, and when I dig into the problem was a corrupted run of the gem mappability command. Thus, I am afraid that the problem is not in ReadTools but there. That's why I contacted the authors of gem3 to know when they'll include the tool in the new implementation.

Could you try to re-run the gem mappability command for one of your input FASTA, and let me know if it fails in the exact same chromosomal position? In that case, that means that it might be a ReadTools problem; otherwise, the gem command should be re-run until it does not procude a writing error (that's the reason of the null character).

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