diff --git a/docs/functional-annotation/viromes.md b/docs/functional-annotation/viromes.md index d3d1c29..c64f3ca 100644 --- a/docs/functional-annotation/viromes.md +++ b/docs/functional-annotation/viromes.md @@ -26,7 +26,7 @@ All these tools and the necessary databases have been preinstalled and are ready ## Step 1 - Initial virus discovery -First, we run **geNomad** to identify viral sequences in our data. We use the ``--enable-score-calibration`` option to compute false discovery rates, allowing us to set a threshold to achieve a desired proportion of false positives (here we will use an FDR < 0.05). Further, we add the use the ``--cleanup`` flag to remove intermediate files and the ``--disable-find-proviruses`` option to avoid geNomad performing an initial prunning to remove potential contaminant host regions from proviral sequences (we will do this in the next step with CheckV). +First, we will run **geNomad** to identify viral sequences in our dataset. We will utilize the ``--enable-score-calibration`` option to compute false discovery rates (FDR). For this practical exercise, no specific FDR thresholds will be applied. Further, we will add the ``--cleanup`` flag to remove intermediate files and the ``--disable-find-proviruses`` option to avoid geNomad performing an initial prunning to remove potential contaminant host regions from proviral sequences (we will do this in the next step with CheckV). **Note:** The following command takes about 10 minutes. You can also continue with the results in ``/data/precomputed/virome/genomad_results/``. ```bash