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Copy pathprimer3_settings.txt
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primer3_settings.txt
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PRIMER_FIRST_BASE_INDEX 1
PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT 1
PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT 0
PRIMER_PICK_LEFT_PRIMER 1
PRIMER_PICK_INTERNAL_OLIGO 0
PRIMER_PICK_RIGHT_PRIMER 1
PRIMER_LIBERAL_BASE 1
PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS 0
PRIMER_LOWERCASE_MASKING 0
PRIMER_PICK_ANYWAY 0
PRIMER_EXPLAIN_FLAG 1
PRIMER_MASK_TEMPLATE 0
PRIMER_TASK generic
PRIMER_MASK_FAILURE_RATE 0.1
PRIMER_MASK_5P_DIRECTION 1
PRIMER_MASK_3P_DIRECTION 0
PRIMER_MIN_QUALITY 0
PRIMER_MIN_END_QUALITY 0
PRIMER_QUALITY_RANGE_MIN 0
PRIMER_QUALITY_RANGE_MAX 100
PRIMER_MIN_SIZE 18
PRIMER_OPT_SIZE 20
PRIMER_MAX_SIZE 23
PRIMER_MIN_TM 55.0
PRIMER_OPT_TM 59.0
PRIMER_MAX_TM 62.0
PRIMER_PAIR_MAX_DIFF_TM 5.0
PRIMER_TM_FORMULA 1
PRIMER_PRODUCT_MIN_TM -1000000.0
PRIMER_PRODUCT_OPT_TM 0.0
PRIMER_PRODUCT_MAX_TM 1000000.0
PRIMER_MIN_GC 30.0
PRIMER_OPT_GC_PERCENT 50.0
PRIMER_MAX_GC 70.0
PRIMER_NUM_RETURN 5
PRIMER_MAX_END_STABILITY 9.0
PRIMER_MAX_LIBRARY_MISPRIMING 12.00
PRIMER_PAIR_MAX_LIBRARY_MISPRIMING 20.00
PRIMER_MAX_SELF_ANY_TH 45.0
PRIMER_MAX_SELF_END_TH 35.0
PRIMER_PAIR_MAX_COMPL_ANY_TH 45.0
PRIMER_PAIR_MAX_COMPL_END_TH 35.0
PRIMER_MAX_HAIRPIN_TH 24.0
PRIMER_MAX_SELF_ANY 8.00
PRIMER_MAX_SELF_END 3.00
PRIMER_PAIR_MAX_COMPL_ANY 8.00
PRIMER_PAIR_MAX_COMPL_END 3.00
PRIMER_MAX_TEMPLATE_MISPRIMING_TH 40.00
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING_TH 70.00
PRIMER_MAX_TEMPLATE_MISPRIMING 12.00
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING 24.00
PRIMER_MAX_NS_ACCEPTED 0
PRIMER_MAX_POLY_X 4
PRIMER_INSIDE_PENALTY -1.0
PRIMER_OUTSIDE_PENALTY 0
PRIMER_GC_CLAMP 0
PRIMER_MAX_END_GC 5
PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE 3
PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE 3
PRIMER_MIN_5_PRIME_OVERLAP_OF_JUNCTION 7
PRIMER_MIN_3_PRIME_OVERLAP_OF_JUNCTION 4
PRIMER_SALT_MONOVALENT 50.0
PRIMER_SALT_CORRECTIONS 1
PRIMER_SALT_DIVALENT 1.5
PRIMER_DNTP_CONC 0.6
PRIMER_DNA_CONC 50.0
PRIMER_SEQUENCING_SPACING 500
PRIMER_SEQUENCING_INTERVAL 250
PRIMER_SEQUENCING_LEAD 50
PRIMER_SEQUENCING_ACCURACY 20
PRIMER_WT_SIZE_LT 1.0
PRIMER_WT_SIZE_GT 1.0
PRIMER_WT_TM_LT 1.0
PRIMER_WT_TM_GT 1.0
PRIMER_WT_GC_PERCENT_LT 0.0
PRIMER_WT_GC_PERCENT_GT 0.0
PRIMER_WT_SELF_ANY_TH 0.0
PRIMER_WT_SELF_END_TH 0.0
PRIMER_WT_HAIRPIN_TH 0.0
PRIMER_WT_TEMPLATE_MISPRIMING_TH 0.0
PRIMER_WT_SELF_ANY 0.0
PRIMER_WT_SELF_END 0.0
PRIMER_WT_TEMPLATE_MISPRIMING 0.0
PRIMER_WT_NUM_NS 0.0
PRIMER_WT_LIBRARY_MISPRIMING 0.0
PRIMER_WT_SEQ_QUAL 0.0
PRIMER_WT_END_QUAL 0.0
PRIMER_WT_POS_PENALTY 0.0
PRIMER_WT_END_STABILITY 0.0
PRIMER_WT_MASK_FAILURE_RATE 0.0
PRIMER_PAIR_WT_PRODUCT_SIZE_LT 0.0
PRIMER_PAIR_WT_PRODUCT_SIZE_GT 0.0
PRIMER_PAIR_WT_PRODUCT_TM_LT 0.0
PRIMER_PAIR_WT_PRODUCT_TM_GT 0.0
PRIMER_PAIR_WT_COMPL_ANY_TH 0.0
PRIMER_PAIR_WT_COMPL_END_TH 0.0
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING_TH 0.0
PRIMER_PAIR_WT_COMPL_ANY 0.0
PRIMER_PAIR_WT_COMPL_END 0.0
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING 0.0
PRIMER_PAIR_WT_DIFF_TM 0.0
PRIMER_PAIR_WT_LIBRARY_MISPRIMING 0.0
PRIMER_PAIR_WT_PR_PENALTY 1.0
PRIMER_PAIR_WT_IO_PENALTY 0.0
PRIMER_INTERNAL_MIN_SIZE 18
PRIMER_INTERNAL_OPT_SIZE 20
PRIMER_INTERNAL_MAX_SIZE 27
PRIMER_INTERNAL_MIN_TM 57.0
PRIMER_INTERNAL_OPT_TM 60.0
PRIMER_INTERNAL_MAX_TM 63.0
PRIMER_INTERNAL_MIN_GC 20.0
PRIMER_INTERNAL_OPT_GC_PERCENT 50.0
PRIMER_INTERNAL_MAX_GC 80.0
PRIMER_INTERNAL_MAX_SELF_ANY_TH 47.00
PRIMER_INTERNAL_MAX_SELF_END_TH 47.00
PRIMER_INTERNAL_MAX_HAIRPIN_TH 47.00
PRIMER_INTERNAL_MAX_SELF_ANY 12.00
PRIMER_INTERNAL_MAX_SELF_END 12.00
PRIMER_INTERNAL_MIN_QUALITY 0
PRIMER_INTERNAL_MAX_NS_ACCEPTED 0
PRIMER_INTERNAL_MAX_POLY_X 5
PRIMER_INTERNAL_MAX_LIBRARY_MISHYB 12.00
PRIMER_INTERNAL_SALT_MONOVALENT 50.0
PRIMER_INTERNAL_DNA_CONC 50.0
PRIMER_INTERNAL_SALT_DIVALENT 1.5
PRIMER_INTERNAL_DNTP_CONC 0.0
PRIMER_INTERNAL_WT_SIZE_LT 1.0
PRIMER_INTERNAL_WT_SIZE_GT 1.0
PRIMER_INTERNAL_WT_TM_LT 1.0
PRIMER_INTERNAL_WT_TM_GT 1.0
PRIMER_INTERNAL_WT_GC_PERCENT_LT 0.0
PRIMER_INTERNAL_WT_GC_PERCENT_GT 0.0
PRIMER_INTERNAL_WT_SELF_ANY_TH 0.0
PRIMER_INTERNAL_WT_SELF_END_TH 0.0
PRIMER_INTERNAL_WT_HAIRPIN_TH 0.0
PRIMER_INTERNAL_WT_SELF_ANY 0.0
PRIMER_INTERNAL_WT_SELF_END 0.0
PRIMER_INTERNAL_WT_NUM_NS 0.0
PRIMER_INTERNAL_WT_LIBRARY_MISHYB 0.0
PRIMER_INTERNAL_WT_SEQ_QUAL 0.0
PRIMER_INTERNAL_WT_END_QUAL 0.0