EasyFig 2.1 'A selected file does not exist'

[wewew47]

Hi,

I have been trying to use Easyfig2.1 (the latest version has in our experience not cooperated as well with our hardware - running on Windows 10) to generate some alignments. I add genbank annotation files and click to generate blastn files, which works okay and the files appear in the UI. I then go to create the figure but consistently get an error message stating 'A selected file does not exist', referring to the blast files because when I run without any of those files it is okay.

I am trying to figure out the problem but have failed to make any progress. I have tried going to the file location stated in the UI (once generated, the file path is shown in the UI for each blast file), but there are no easyfig files in that pathway with the same name. The UI name is "C:/Users/pathway/12.easyfig.out" etc but in the folder all that is generated is files with the name 1.easyfig.fa, 2.easyfig,fa etc.

Do you have any suggestions? Any help would be greatly appreciated!
I have attached some images which hopefully illustrate my issue a little more. The console image is of when the blastn is running.

Best,

[restrictione]

this problem i m facing since 2015..now its 2021..
can i get this fixed somehow???
please help
i am using the latest version 2.2.5
still facing the same issue .... blast .out flie does not exist

[wewew47]

Hi restictione,

I never managed to fix this issue on a windows computer but if you're desperate, you could try installing EasyFig on a Linux device, or set up a linux virtual machine if you don't have a linux computer. I did the latter and whilst a little annoying, it got the software working at least!

[luisafmc]

Hi, I had the same problem and I found a very simple solution. Just save the blast file and output file in the same folder of the program. And finally, it works! I am running the program in windows

[hanrong-chen]

I had the same problem on a Windows laptop. I had saved my blast+ executables under "Program Files". But the pathname cannot have any spaces (see #49). Moving the blast+ executables elsewhere (e.g. in C drive) fixed the issue for me.