PLAYRdesign for mouse sequences

[marieaguirre]

Hello,

I would like to use the PLAYRdesign on mouse genome, so i did the same with mouse data bases but it doesn't work ? The script is out of bounds.

Marie

[pfgherardini]

Hi

Please include a sample of the files that you are using. This is most likely a problem with the format of the FASTA headers

[marieaguirre]

I use this sequence;

gi|20135164|ref|NM_005665.3|
GAGGTCAGTAGTTTCCTCATCACGGCTGCCTCACTTTCTTCCAGTTCAACCAGCCCTGACAGCTCCCAAG
CCTTGAGTCACAGAGAGTTGGGAGCCACTCTGCTCACAAACAGCGTCGTAGAACTACTGCAGGATTAAGT
TTACCTCAAAGATGGGAGAATTCAAGGTGGATAAGTTCAACATTGAAGATTTCTTCAGTGGAGATCTTGA
TATTTTCAATTATAGCTCTGGCATGCCCTCTATTCTGCCAGATGCTGTCCCATGCCACTCAGAGAACCTG
GAAATCAACAGTTATGCTGTGGTTGTAATATACGTCCTGGTGACTCTGCTGAGCCTTGTGGGGAACTCCT
TGGTGATGCTGGTCATCTTATACAACCGGAGCACCTGCTCTGTCACCGATGTCTACCTGCTGAACCTGGC
CATTGCTGACCTGTTCTTTGCCCTGACCTTGCCTGTCTGGGCTGCATCTAAAGTAAATGGATGGACTTTT
GGCTCAACCCTGTGCAAGATATTCTCATACGTGAAGGAGGTTACCTTCTACAGCAGTGTTCTGCTACTAG
CCTGCATCAGCATGGACCGCTACCTGGCCATTGTACATGCCACAAGTACACTGATCCAGAAGAGACACTT
GGTCAAGTTTGTGTGCATAGCCATGTGGTTACTATCAGTAATTCTGGCCCTGCCCATCTTAATTCTACGA
AATCCTGTTAAGGTAAACCTTTCTACCTTAGTCTGCTATGAGGATGTAGGTAACAATACATCCCGTTTGA

But we run the software with this sequence on human data base just to check if the Fasta was okay, and it works.

[pfgherardini]

Have you managed to fix this problem? If not make sure that the FASTA headers of your mouse sequence database (i.e. the database file not the input sequence) are correct.

If you still have problems, please share with me a dropbox folder with all the sequences