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v2.0.0: Pathotyping and Shiga toxin typing

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@kbessonov1984 kbessonov1984 released this 12 Dec 12:30
21f2cbd
  • Updated species identification module now based on GTDB + custom Escherichia and Shigella sketch covering all known bacterial species
  • Implemented pathotyping covering 7 DEC Escherichia coli pathotypes (DAEC, EAEC, EHEC, EIEC, EPEC, ETEC and STEC) supporting simultaneous presence of multiple signatures (e.g. ETEC/STEC). Note that EHEC is reported as EHEC-STEC as this is a more severe subtype of STEC.
  • Implemented Shiga 1 and 2 toxin typing supporting multiple toxin signatures present in a single sample.
    • A total of 4 stx1 subtypes are supported: stx1a, stx1c, stx1d and stx1e.
    • A total of 15 stx2 subtypes are supported: stx2a, stx2b, stx2c, stx2d, stx2e, stx2f, stx2g ,stx2h, stx2i, stx2j, stx2k, stx2l, stx2m, stx2n, stx2o.
  • new database of pathotypes and toxins in JSON clear transparent format composed of the key virulence factors based on both BioNumerics and literature sources
  • support for gzip compressed inputs fastq.gz and fasta.gz saving storage and increasing versatility
  • other toxin typing covering enterohemolysin A (ehxA), hemolysin E (hlyE), hemolysin A (hlyA)
  • support for long raw reads improving mapping capabilities of bowtie2