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Updated species identification module now based on GTDB + custom Escherichia and Shigella sketch covering all known bacterial species
Implemented pathotyping covering 7 DEC Escherichia coli pathotypes (DAEC, EAEC, EHEC, EIEC, EPEC, ETEC and STEC) supporting simultaneous presence of multiple signatures (e.g. ETEC/STEC). Note that EHEC is reported as EHEC-STEC as this is a more severe subtype of STEC.
Implemented Shiga 1 and 2 toxin typing supporting multiple toxin signatures present in a single sample.
A total of 4 stx1 subtypes are supported: stx1a, stx1c, stx1d and stx1e.
A total of 15 stx2 subtypes are supported: stx2a, stx2b, stx2c, stx2d, stx2e, stx2f, stx2g ,stx2h, stx2i, stx2j, stx2k, stx2l, stx2m, stx2n, stx2o.
new database of pathotypes and toxins in JSON clear transparent format composed of the key virulence factors based on both BioNumerics and literature sources
support for gzip compressed inputs fastq.gz and fasta.gz saving storage and increasing versatility
other toxin typing covering enterohemolysin A (ehxA), hemolysin E (hlyE), hemolysin A (hlyA)
support for long raw reads improving mapping capabilities of bowtie2