diff --git a/ci/conda_env_python.txt b/ci/conda_env_python.txt
index 42143eceb3f2e..9a48f26b79c6e 100644
--- a/ci/conda_env_python.txt
+++ b/ci/conda_env_python.txt
@@ -18,8 +18,7 @@
 # Don't add pandas here, because it is not a mandatory test dependency
 
 # Not a direct dependency of s3fs, but needed for our s3fs fixture
-# (temporary upper bound because of GH-45305)
-boto3<1.36
+boto3
 cffi
 cython>=3
 cloudpickle
diff --git a/ci/scripts/install_minio.sh b/ci/scripts/install_minio.sh
index 6f9701ab5a150..8685ced0bd1ab 100755
--- a/ci/scripts/install_minio.sh
+++ b/ci/scripts/install_minio.sh
@@ -63,7 +63,7 @@ if [ "${version}" != "latest" ]; then
 fi
 
 # Use specific versions for minio server and client to avoid CI failures on new releases.
-minio_version="minio.RELEASE.2024-09-13T20-26-02Z"
+minio_version="minio.RELEASE.2025-01-20T14-49-07Z"
 mc_version="mc.RELEASE.2024-09-16T17-43-14Z"
 
 download()