From da19dc3e02846859d7a52c6fda73a0c02b7503be Mon Sep 17 00:00:00 2001 From: pwwang <1188067+pwwang@users.noreply.github.com> Date: Wed, 27 Sep 2023 14:58:35 -0700 Subject: [PATCH] =?UTF-8?q?=E2=9C=A8=20Allow=20passing=20vcf=20as=202nd=20?= =?UTF-8?q?arg=20to=20macros=20(#45)=20(#46)?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit * ✨ Allow passing vcf as 2nd arg to macros (#45) * ⬆️ Update dependencies * 🔖 0.6.0 --- docs/CHANGELOG.md | 5 + docs/macros.md | 13 + ...ele-frequency-on-chromosome-12.density.png | Bin 34364 -> 34303 bytes ...ncy-on-each-chromosome-boxplot.boxplot.png | Bin 19977 -> 20285 bytes ...le-frequency-on-each-chromosome.violin.png | Bin 42898 -> 47121 bytes .../depths-between-sample-1-and-2.scatter.png | Bin 27576 -> 28557 bytes ...le-frequency-on-chromosome-1.histogram.png | Bin 12825 -> 13348 bytes examples/gq-vs-depth-sample-1.scatter.png | Bin 19867 -> 20253 bytes ...otypes-on-each-chromosome-sample-1.col.png | Bin 19956 -> 19883 bytes ...er-of-substitutions-of-snps-passed.col.png | Bin 18519 -> 18792 bytes .../number-of-substitutions-of-snps.col.png | Bin 17833 -> 18229 bytes ...ariants-on-each-chromosome-first-5.col.png | Bin 14363 -> 14631 bytes ...riants-on-each-chromosome-modified.col.png | Bin 17193 -> 17362 bytes ...ber-of-variants-on-each-chromosome.col.png | Bin 15518 -> 16107 bytes ...uency-distribution-0-05-0-95.histogram.png | Bin 14283 -> 14404 bytes ...llele-frequency-distribution.histogram.png | Bin 12634 -> 13178 bytes .../types-of-variants-on-chromosome-1.pie.png | Bin 16048 -> 16286 bytes ...pes-of-variants-on-each-chromosome.col.png | Bin 16984 -> 17159 bytes .../types-of-variants-on-whole-genome.col.png | Bin 17499 -> 17613 bytes .../types-of-variants-on-whole-genome.pie.png | Bin 14384 -> 16796 bytes poetry.lock | 1402 +++++++++-------- pyproject.toml | 9 +- tests/test_formula.py | 74 +- tests/test_instance.py | 2 +- tests/test_macros.py | 20 + vcfstats/__init__.py | 2 +- vcfstats/__main__.py | 2 +- vcfstats/cli.py | 2 +- vcfstats/formula.py | 29 +- vcfstats/instance.py | 4 +- vcfstats/macros.py | 8 + 31 files changed, 816 insertions(+), 756 deletions(-) diff --git a/docs/CHANGELOG.md b/docs/CHANGELOG.md index 01dd5ff..91e5ce7 100644 --- a/docs/CHANGELOG.md +++ b/docs/CHANGELOG.md @@ -1,5 +1,10 @@ # Change Log +## 0.6.0 + +- ✨ Allow passing vcf as 2nd arg to macros (#45) +- ⬆️ Update dependencies + ## 0.5.0 - ➕ Use argx instead of pyparam diff --git a/docs/macros.md b/docs/macros.md index 835a5a1..9d6f750 100644 --- a/docs/macros.md +++ b/docs/macros.md @@ -45,6 +45,19 @@ def GTTYPEs(variant) To get the genotype in sample 1 in formula: `GTTYPEs{0}`. You can also use sample name as well: `GTTYPEs{some sample}` +It's also allowed to pass `vcf` (the instance of `cyvcf.VCF`) as the second argument to the macro. For example: + +```python +from vcf.macros import cont + +@cont +def MIXED_INFO(variant, vcf): + ... +``` + +Check the [API documentation](https://brentp.github.io/cyvcf2/docstrings.html) of `cyvcf2` to see what information we can get from `vcf`. + + ## Macros with filters `aggregation`s have different syntax for filters. 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"sphinx-lint"] -testing = ["flake8 (<5)", "func-timeout", "jaraco.functools", "jaraco.itertools", "more-itertools", "pytest (>=6)", "pytest-black (>=0.3.7)", "pytest-checkdocs (>=2.4)", "pytest-cov", "pytest-enabler (>=1.3)", "pytest-flake8", "pytest-mypy (>=0.9.1)"] +docs = ["furo", "jaraco.packaging (>=9.3)", "jaraco.tidelift (>=1.4)", "rst.linker (>=1.9)", "sphinx (<7.2.5)", "sphinx (>=3.5)", "sphinx-lint"] +testing = ["big-O", "jaraco.functools", "jaraco.itertools", "more-itertools", "pytest (>=6)", "pytest-black (>=0.3.7)", "pytest-checkdocs (>=2.4)", "pytest-cov", "pytest-enabler (>=2.2)", "pytest-ignore-flaky", "pytest-mypy (>=0.9.1)", "pytest-ruff"] [metadata] lock-version = "2.0" python-versions = "^3.8" -content-hash = "1534cb7af3e30994b9d31974de0b3ede753f81656374e70beea96293504f46cb" +content-hash = "b8cd3a0a1ec4dddaee488af9ec7ef67bb339e1e36c24d55d8cba55595d747970" diff --git a/pyproject.toml b/pyproject.toml index 1b60333..594cb22 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -4,7 +4,7 @@ build-backend = "poetry.masonry.api" [tool.poetry] name = "vcfstats" -version = "0.5.0" +version = "0.6.0" description = "Powerful statistics for VCF files" authors = [ "pwwang ",] license = "MIT" @@ -17,12 +17,11 @@ include = ["vcfstats/args.toml"] python = "^3.8" cyvcf2 = "^0.30" lark-parser = "^0.12" -plotnine = "^0.10" -plotnine-prism = "^0.2" +plotnine-prism = "^0.3" python-slugify = "^8" -datar = { version = "^0.11", extras = ["pandas"] } +datar = { version = "^0.13", extras = ["pandas"] } py = "^1.10" -argx = "^0.1.0" +argx = "^0.2" rich = "^13" [tool.poetry.dev-dependencies] diff --git a/tests/test_formula.py b/tests/test_formula.py index b07031d..3b146a4 100644 --- a/tests/test_formula.py +++ b/tests/test_formula.py @@ -109,28 +109,28 @@ def test_term_init(): def test_term_run(variants): term = Term("FILTER", "PASS") - assert term.run(variants[0], passed=True) == False - assert term.run(variants[5], passed=True) == ["PASS"] + assert term.run(variants[0], None, passed=True) == False + assert term.run(variants[5], None, passed=True) == ["PASS"] term = Term("FILTER2") - assert term.run(variants[5], passed=True) == False + assert term.run(variants[5], None, passed=True) == False term = Term("GTTYPEs", None, ["0"]) term.set_samples(variants[-1]) - assert term.run(variants[0], passed=False) == ["HOM_REF"] + assert term.run(variants[0], None, passed=False) == ["HOM_REF"] term = Term("AAF", [0.126, None]) # .125 - assert term.run(variants[0], passed=False) == False - assert term.run(variants[2], passed=False) == [0.25] + assert term.run(variants[0], None, passed=False) == False + assert term.run(variants[2], None, passed=False) == [0.25] term = Term("AAF", [None, 0.24]) # .25 - assert term.run(variants[0], passed=False) == [0.125] - assert term.run(variants[2], passed=False) == False + assert term.run(variants[0], None, passed=False) == [0.125] + assert term.run(variants[2], None, passed=False) == False term = Term("FILTER", "PASS") - assert term.run(variants[0], passed=False) == False - assert term.run(variants[5], passed=False) == ["PASS"] + assert term.run(variants[0], None, passed=False) == False + assert term.run(variants[5], None, passed=False) == ["PASS"] def test_aggr_init(): @@ -194,54 +194,54 @@ def test_aggr_run(variants): aggr = Aggr( "COUNT", One(), filter=Term("FILTER", ["PASS"]), group=Term("VARTYPE") ) - aggr.run(variants[0], passed=True) + aggr.run(variants[0], None, passed=True) assert len(aggr.cache) == 0 aggr2 = Aggr("COUNT", One(), filter=Term("FILTER", ["PASS"])) with pytest.raises(RuntimeError): - aggr2.run(variants[5], passed=True) + aggr2.run(variants[5], None, passed=True) aggr3 = Aggr( "COUNT", One(), filter=Term("FILTER", ["PASS"]), group=Term("FILTER2") ) - aggr3.run(variants[5], passed=False) + aggr3.run(variants[5], None, passed=False) assert len(aggr3.cache) == 0 aggr4 = Aggr("COUNT", One(), Term("GTTYPEs")) with pytest.raises(ValueError): - aggr4.run(variants[0], passed=False) + aggr4.run(variants[0], None, passed=False) aggr5 = Aggr("COUNT", One(), Term("VARTYPE")) - aggr5.run(variants[0], passed=False) + aggr5.run(variants[0], None, passed=False) assert aggr5.cache == {"snp": [1]} - aggr5.run(variants[1], passed=False) + aggr5.run(variants[1], None, passed=False) assert aggr5.cache == {"snp": [1, 1]} - aggr5.run(variants[3], passed=False) + aggr5.run(variants[3], None, passed=False) assert aggr5.cache == {"snp": [1, 1], "indel": [1]} assert aggr5.dump() == {"snp": 2, "indel": 1} aggr5.cache.clear() aggr5.setxgroup(Term("FILTER", None)) - aggr5.run(variants[0], passed=False) + aggr5.run(variants[0], None, passed=False) assert aggr5.cache == {"MinMQ": {"snp": [1]}} aggr5.cache.clear() aggr5.setxgroup(Term("FILTER2", None)) - aggr5.run(variants[0], passed=False) + aggr5.run(variants[0], None, passed=False) assert aggr5.cache == {"MinMQ": {"snp": [1]}} - aggr5.run(variants[5], passed=False) + aggr5.run(variants[5], None, passed=False) assert aggr5.cache == {"MinMQ": {"snp": [1]}} - aggr5.run(variants[1], passed=False) + aggr5.run(variants[1], None, passed=False) assert aggr5.cache == {"MinMQ": {"snp": [1, 1]}} assert aggr5.dump() == {"MinMQ": [(2, "snp")]} aggr5.setxgroup(Term("GTTYPEs", ["HOM_REF", "HET"])) with pytest.raises(ValueError): - aggr5.run(variants[0], passed=False) + aggr5.run(variants[0], None, passed=False) aggr6 = Aggr("MEAN", Term("AAF", [".2", None]), Term("CHROM")) - aggr6.run(variants[0], passed=False) # .125 + aggr6.run(variants[0], None, passed=False) # .125 assert len(aggr6.cache) == 0 @@ -272,14 +272,14 @@ def test_formula_run(variants): data = [] fmula = Formula("AFs{0,1} ~ GTTYPEs{0-2}", variants[-1], False, "title") with pytest.raises(RuntimeError): - fmula.run(variants[0], data.append, data.extend) + fmula.run(variants[0], None, data.append, data.extend) fmula = Formula("FILTER2 ~ CHROM", variants[-1], False, "title") - fmula.run(variants[5], data.append, data.extend) + fmula.run(variants[5], None, data.append, data.extend) assert data == [] fmula = Formula("GTTYPEs ~ CHROM", variants[-1], False, "title") - fmula.run(variants[0], data.append, data.extend) + fmula.run(variants[0], None, data.append, data.extend) assert data == [ ("HOM_REF", "1"), ("HOM_REF", "1"), @@ -289,7 +289,7 @@ def test_formula_run(variants): data = [] fmula = Formula("CHROM ~ GTTYPEs", variants[-1], False, "title") - fmula.run(variants[0], data.append, data.extend) + fmula.run(variants[0], None, data.append, data.extend) assert data == [ ("1", "HOM_REF"), ("1", "HOM_REF"), @@ -304,8 +304,8 @@ def test_formula_run(variants): False, "title", ) - fmula.run(variants[0], data.append, data.extend) - fmula.run(variants[1], data.append, data.extend) + fmula.run(variants[0], None, data.append, data.extend) + fmula.run(variants[1], None, data.append, data.extend) assert data == [] fmula.done(data.append, data.extend) assert data == [(2, 2, "1")] @@ -317,7 +317,7 @@ def test_formula_run(variants): False, "title", ) - fmula.run(variants[0], data.append, data.extend) + fmula.run(variants[0], None, data.append, data.extend) assert data == [] with pytest.raises(ValueError): @@ -329,8 +329,8 @@ def test_formula_run(variants): ) fmula = Formula("COUNT(1) ~ CHROM", variants[-1], False, "title") - fmula.run(variants[0], data.append, data.extend) - fmula.run(variants[1], data.append, data.extend) + fmula.run(variants[0], None, data.append, data.extend) + fmula.run(variants[1], None, data.append, data.extend) assert data == [] assert fmula.Y.cache == {"1": [1, 1]} fmula.done(data.append, data.extend) @@ -338,15 +338,15 @@ def test_formula_run(variants): fmula = Formula("CHROM ~ COUNT(1)", variants[-1], False, "title") with pytest.raises(TypeError): - fmula.run(variants[0], data.append, data.extend) + fmula.run(variants[0], None, data.append, data.extend) data = [] fmula = Formula( "COUNT(1, group = VARTYPE) ~ CHROM", variants[-1], False, "title" ) - fmula.run(variants[0], data.append, data.extend) - fmula.run(variants[1], data.append, data.extend) - fmula.run(variants[2], data.append, data.extend) - fmula.run(variants[3], data.append, data.extend) + fmula.run(variants[0], None, data.append, data.extend) + fmula.run(variants[1], None, data.append, data.extend) + fmula.run(variants[2], None, data.append, data.extend) + fmula.run(variants[3], None, data.append, data.extend) fmula.done(data.append, data.extend) assert data == [(3, "1", "snp"), (1, "1", "indel")] diff --git a/tests/test_instance.py b/tests/test_instance.py index 4044e42..67abba0 100644 --- a/tests/test_instance.py +++ b/tests/test_instance.py @@ -105,7 +105,7 @@ def test_one_iterate(tmp_path): False, ) with pytest.raises(AttributeError): - instance.iterate(None) + instance.iterate(None, None) # def test_summarize(instance): diff --git a/tests/test_macros.py b/tests/test_macros.py index c4adf3a..646ff9a 100644 --- a/tests/test_macros.py +++ b/tests/test_macros.py @@ -4,6 +4,7 @@ from cyvcf2 import VCF from vcfstats.macros import ( + cat, _ONE, TITV, VARTYPE, @@ -26,6 +27,12 @@ HERE = Path(__file__).parent.resolve() +@cat +def mixedinfo(variant, vcf): + """Global position of the variant""" + return vcf.raw_header[:6] + variant.CHROM + + @pytest.fixture(scope="module") def variants(): vcf = VCF( @@ -35,6 +42,19 @@ def variants(): return list(vcf) +@pytest.fixture(scope="module") +def variants_vcf(): + vcf = VCF( + str(HERE.parent.joinpath("examples", "sample.vcf")), + gts012=True, + ) + return list(vcf), vcf + + +def test_variant_vcf(variants_vcf): + assert mixedinfo(variants_vcf[0][0], variants_vcf[1]) == "##file1" + + def test_vartype(variants): assert VARTYPE(variants[0]) == "snp" assert VARTYPE(variants[1]) == "snp" diff --git a/vcfstats/__init__.py b/vcfstats/__init__.py index 9215951..85f989e 100644 --- a/vcfstats/__init__.py +++ b/vcfstats/__init__.py @@ -3,4 +3,4 @@ from . import macros -__version__ = "0.5.0" +__version__ = "0.6.0" diff --git a/vcfstats/__main__.py b/vcfstats/__main__.py index 99f7254..3fcdfb2 100644 --- a/vcfstats/__main__.py +++ b/vcfstats/__main__.py @@ -1,5 +1,5 @@ """Main entrance for `python -m vcfstats`""" - +# pragma: no cover from .cli import main if __name__ == "__main__": diff --git a/vcfstats/cli.py b/vcfstats/cli.py index b8d1866..b20c984 100644 --- a/vcfstats/cli.py +++ b/vcfstats/cli.py @@ -189,7 +189,7 @@ def main(): for i, variant in enumerate(vcf): for instance in ones: # save entries, cache aggr - instance.iterate(variant) + instance.iterate(variant, vcf) if i % 10000 == 0: # pragma: no cover logger.debug("- %s variants read.", i) logger.info( diff --git a/vcfstats/formula.py b/vcfstats/formula.py index a53e913..8cbc0e9 100644 --- a/vcfstats/formula.py +++ b/vcfstats/formula.py @@ -124,11 +124,14 @@ def __eq__(self, other): def __ne__(self, other): return not self.__eq__(other) - def run(self, variant, passed): + def run(self, variant, vcf, passed): """Run the variant""" if passed and variant.FILTER: return False - value = self.term["func"](variant) + if self.term["nargs"] == 2: + value = self.term["func"](variant, vcf) + else: + value = self.term["func"](variant) if value is False or value is None: return False @@ -206,9 +209,9 @@ def setxgroup(self, xvar): else: self.xgroup = xvar - def run(self, variant, passed): + def run(self, variant, vcf, passed): """Run each variant""" - if self.filter and self.filter.run(variant, passed) is False: + if self.filter and self.filter.run(variant, vcf, passed) is False: return if not self.group: @@ -216,11 +219,11 @@ def run(self, variant, passed): "No group specified, don't know how to aggregate." ) - group = self.group.run(variant, passed) + group = self.group.run(variant, vcf, passed) if group is False: return - value = self.term.run(variant, passed) + value = self.term.run(variant, vcf, passed) if value is False: return @@ -233,7 +236,7 @@ def run(self, variant, passed): xgroup = None if self.xgroup: - xgroup = self.xgroup.run(variant, passed) + xgroup = self.xgroup.run(variant, vcf, passed) if xgroup is False: return if len(xgroup) > 1 and len(value) != len(xgroup): @@ -312,12 +315,12 @@ def __init__(self, formula, samples, passed, title): ): self.passed = False - def run(self, variant, data_append, data_extend): + def run(self, variant, vcf, data_append, data_extend): """Run each variant""" if isinstance(self.Y, Term) and isinstance(self.X, Term): yvar, xvar = ( - self.Y.run(variant, self.passed), - self.X.run(variant, self.passed), + self.Y.run(variant, vcf, self.passed), + self.X.run(variant, vcf, self.passed), ) if yvar is False or xvar is False: return @@ -336,10 +339,10 @@ def run(self, variant, data_append, data_extend): data_extend(((yvar[i], rvar) for i, rvar in enumerate(xvar))) elif isinstance(self.Y, Aggr) and isinstance(self.X, Aggr): - self.Y.run(variant, self.passed) - self.X.run(variant, self.passed) + self.Y.run(variant, vcf, self.passed) + self.X.run(variant, vcf, self.passed) elif isinstance(self.Y, Aggr) and isinstance(self.X, Term): - self.Y.run(variant, self.passed) + self.Y.run(variant, vcf, self.passed) else: raise TypeError( "Cannot do 'TERM ~ AGGREGATION'. " diff --git a/vcfstats/instance.py b/vcfstats/instance.py index 67af94f..a15843c 100644 --- a/vcfstats/instance.py +++ b/vcfstats/instance.py @@ -175,10 +175,10 @@ def __init__( def __del__(self): del self.data - def iterate(self, variant): + def iterate(self, variant, vcf): """Iterate over each variant""" # Y - self.formula.run(variant, self.data.append, self.data.extend) + self.formula.run(variant, vcf, self.data.append, self.data.extend) def summarize(self): """Calculate the aggregations""" diff --git a/vcfstats/macros.py b/vcfstats/macros.py index eb68882..79ec50c 100644 --- a/vcfstats/macros.py +++ b/vcfstats/macros.py @@ -1,10 +1,16 @@ """Builtin marcros for vcfstats""" import warnings +from inspect import signature from functools import partial from .utils import MACROS +def _nargs(func): + """Get the number of arguments of a function""" + return len(signature(func).parameters) + + def categorical(func=None, alias=None, _name=None): """Categorical decorator""" if alias: @@ -14,6 +20,7 @@ def categorical(func=None, alias=None, _name=None): MACROS[funcname] = {} MACROS[funcname]["func"] = MACROS[funcname].get("func", func) MACROS[funcname]["type"] = "categorical" + MACROS[funcname]["nargs"] = _nargs(func) if _name: MACROS[_name] = MACROS[funcname] return MACROS[funcname]["func"] @@ -28,6 +35,7 @@ def continuous(func=None, alias=None, _name=None): MACROS[funcname] = {} MACROS[funcname]["func"] = MACROS[funcname].get("func", func) MACROS[funcname]["type"] = "continuous" + MACROS[funcname]["nargs"] = _nargs(func) if _name: MACROS[_name] = MACROS[funcname] return MACROS[funcname]["func"]