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关于seurat计算的问题 #42

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TATABOX99 opened this issue Nov 25, 2024 · 0 comments

TATABOX99 commented Nov 25, 2024

作者您好，你的seurat计算部分使用的：sc.metabolism.Seurat <- function(obj, method = "VISION", imputation = F, ncores = 2, metabolism.type = "KEGG") {

countexp<-obj@assays$RNA@count

在seurat5.0中不可用了，此外考虑到批次效应，是否可以尝试改成：sc.metabolism.Seurat <- function(obj, method = "VISION", imputation = F, ncores = 2, metabolism.type = "KEGG") {

countexp<-obj@assays$RNA$data

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