A graphical user interface-based analysis suite developed by the Biophysics Group at the University of Pretoria, South Africa.
- Performs change point analysis to resolve brightness levels of fluorescence measurements.
- Uses Agglomerative Hierarchical Clustering to group resolved levels.
- Performs fluorescence decay fitting to measure lifetimes.
- Allows filtering and export of analysed brightness/lifetime data.
- Computes second-order correlation functions from time-tagged photon data.
- Displays raster scan images and fluorescence spectral time traces.
- Inability to close measurement file once opened.
- Some systems may encounter missing numxpr module.
- Improved stability.
- Additional conversion tools.
- Additional analysis functionality, such as for fluorescence correlation spectroscopy.
An example file can be downloaded here: https://rb.gy/wtzg0z.
The documentation is available here: https://up-biophysics-sms.readthedocs.io/en/latest/index.html.
This software is to be used only if reference is made to the following paper:
Botha, J.L., van Heerden, B., and Krüger, T.P.J., "Advanced analysis of single-molecule spectroscopic data", Biophysical Reports 4(3), September 2024, https://doi.org/10.1016/j.bpr.2024.100173.