new_library_normalization_revio

cg_lims/EPPs/udf/calculate/base.py

@@ -21,6 +21,7 @@
 from cg_lims.EPPs.udf.calculate.calculate_water_volume_rna import calculate_water_volume_rna
 from cg_lims.EPPs.udf.calculate.get_missing_reads import get_missing_reads
 from cg_lims.EPPs.udf.calculate.library_normalization import library_normalization
+from cg_lims.EPPs.udf.calculate.library_normalization_revio import library_normalization_revio
 from cg_lims.EPPs.udf.calculate.maf_calculate_volume import maf_calculate_volume
 from cg_lims.EPPs.udf.calculate.molar_concentration import molar_concentration
 from cg_lims.EPPs.udf.calculate.novaseq_x_denaturation import novaseq_x_denaturation
@@ -62,6 +63,7 @@ def calculate(ctx):
 calculate.add_command(calculate_average_size_and_set_qc)
 calculate.add_command(novaseq_x_volumes)
 calculate.add_command(library_normalization)
+calculate.add_command(library_normalization_revio)
 calculate.add_command(novaseq_x_denaturation)
 calculate.add_command(qpcr_concentration)
 calculate.add_command(calculate_saphyr_concentration)

cg_lims/EPPs/udf/calculate/library_normalization.py

@@ -6,33 +6,13 @@
 from cg_lims import options
 from cg_lims.exceptions import InvalidValueError, LimsError, MissingValueError
 from cg_lims.get.artifacts import get_artifacts
-from cg_lims.get.udfs import get_udf
+from cg_lims.get.udfs import get_final_concentration, get_artifact_concentration, get_total_volume, get_process_total_volume
 from genologics.entities import Artifact, Process
 
 LOG = logging.getLogger(__name__)
 failed_samples = []
 
 
-def get_final_concentration(process: Process, final_concentration_udf: str) -> float:
-    """Return final concentration value from process."""
-    return float(get_udf(entity=process, udf=final_concentration_udf))
-
-
-def get_artifact_concentration(artifact: Artifact, concentration_udf: str) -> float:
-    """Return concentration value from artifact."""
-    return float(get_udf(entity=artifact, udf=concentration_udf))
-
-
-def get_total_volume(artifact: Artifact, total_volume_udf: str) -> float:
-    """Return total volume value from artifact."""
-    return float(get_udf(entity=artifact, udf=total_volume_udf))
-
-
-def get_process_total_volume(process: Process, total_volume_udf: str) -> Optional[float]:
-    """Return total volume value from process."""
-    return process.udf.get(total_volume_udf)
-
-
 def calculate_sample_volume(
     final_concentration: float, total_volume: float, sample_concentration: float, artifact: Artifact
 ) -> float:

cg_lims/EPPs/udf/calculate/library_normalization_revio.py

@@ -0,0 +1,119 @@
+import logging
+import sys
+from typing import List, Optional
+
+import click
+from cg_lims import options
+from cg_lims.exceptions import InvalidValueError, LimsError, MissingValueError
+from cg_lims.get.artifacts import get_artifacts
+from cg_lims.get.udfs import get_final_concentration, get_artifact_concentration, get_artifact_volume
+from genologics.entities import Artifact, Process
+
+LOG = logging.getLogger(__name__)
+failed_samples = []
+
+
+def calculate_total_volume(
+        final_concentration: float, 
+        sample_volume: float, 
+        sample_concentration: float, 
+        artifact: Artifact, 
+) -> float:
+    """Calculate and return the total volume needed to reach the desired final concentration."""
+    if final_concentration > sample_concentration:
+        error_message: str = (
+            f"The final concentration ({final_concentration} ng/ul) is higher than the original one"
+            f" ({sample_concentration} ng/ul) for sample {artifact.samples[0].id}. No dilution needed."
+        )
+        LOG.error(error_message)
+        global failed_samples
+        failed_samples.append(artifact.name)
+        return sample_volume
+    return (sample_volume * sample_concentration) / final_concentration
+
+
+def calculate_buffer_volume(
+        total_volume: float, sample_volume
+) -> float:
+    """Calculate and return the buffer volume to dilute the sample with to the desired total volume"""
+    return total_volume - sample_volume
+
+
+def set_artifact_volumes(
+    artifacts: List[Artifact],
+    final_concentration: float,
+    total_volume_udf: Optional[str],
+    sample_volume_udf: str,
+    buffer_volume_udf: str,
+    concentration_udf: str,
+) -> None:
+    """Set volume UDFs on artifact level, given a list of artifacts, final concentration, and UDF names."""
+    for artifact in artifacts:
+        sample_concentration: float = get_artifact_concentration(
+            artifact=artifact, concentration_udf=concentration_udf
+        )
+        sample_volume: float = get_artifact_volume(
+            artifact=artifact,
+            sample_volume_udf=sample_volume_udf
+        )
+        total_volume: float = calculate_total_volume(
+            final_concentration=final_concentration,
+            artifact=artifact,
+            sample_volume=sample_volume,
+            sample_concentration=sample_concentration
+        )
+        buffer_volume: float = calculate_buffer_volume(
+            total_volume=total_volume, sample_volume=sample_volume
+        )
+        artifact.udf[buffer_volume_udf] = buffer_volume
+        artifact.udf[total_volume_udf] = total_volume
+        artifact.put()
+
+
+@click.command()
+@options.input()
+@options.sample_udf(help="Name of sample volume UDF.")
+@options.buffer_udf(help="Name of buffer volume UDF.")
+@options.concentration_udf(help="Name of sample concentration UDF.")
+@options.final_concentration_udf(help="Name of final target concentration UDF.")
+@options.total_volume_udf(
+    help="Name of total volume UDF on sample level. Note: Can't be combined with the process level alternative."
+)
+@click.pass_context
+def library_normalization_revio(
+    ctx: click.Context,
+    input: bool,
+    sample_udf: str,
+    buffer_udf: str,
+    concentration_udf: str,
+    final_concentration_udf: str,
+    total_volume_udf: Optional[str] = None,
+) -> None:
+    """Calculate the volumes for dilution for a set concentration with the total available sample volume"""
+
+    LOG.info(f"Running {ctx.command_path} with params: {ctx.params}")
+    process: Process = ctx.obj["process"]
+    artifacts: List[Artifact] = get_artifacts(process=process, input=input)
+    try:
+        final_concentration: float = get_final_concentration(
+            process=process, final_concentration_udf=final_concentration_udf
+        )
+        set_artifact_volumes(
+            artifacts=artifacts,
+            final_concentration=final_concentration,
+            total_volume_udf=total_volume_udf,
+            sample_volume_udf=sample_udf,
+            buffer_volume_udf=buffer_udf,
+            concentration_udf=concentration_udf,
+        )
+        if failed_samples:
+            failed_samples_string = ", ".join(failed_samples)
+            error_message = f"The following artifacts had a lower concentration than targeted: {failed_samples_string}"
+            LOG.info(error_message)
+            raise InvalidValueError(error_message)
+        message: str = "Volumes were successfully calculated."
+        LOG.info(message)
+        click.echo(message)
+    except LimsError as e:
+        LOG.error(e.message)
+        sys.exit(e.message)

cg_lims/get/udfs.py

@@ -5,7 +5,7 @@
 
 from cg_lims.exceptions import MissingUDFsError
 from cg_lims.get.artifacts import get_latest_analyte
-from genologics.entities import Artifact, Entity
+from genologics.entities import Artifact, Entity, Process
 from genologics.lims import Lims
 
 LOG = logging.getLogger(__name__)
@@ -71,3 +71,28 @@ def get_analyte_udf(lims: Lims, sample_id: str, process_types: List[str], udf: s
             f"Couldn't find UDF '{udf}' for artifacts of sample {sample_id} generated by the step {process_types}"
         )
     return value
+
+
+def get_final_concentration(process: Process, final_concentration_udf: str) -> float:
+    """Return final concentration value from process."""
+    return float(get_udf(entity=process, udf=final_concentration_udf))
+
+
+def get_artifact_concentration(artifact: Artifact, concentration_udf: str) -> float:
+    """Return concentration value from artifact."""
+    return float(get_udf(entity=artifact, udf=concentration_udf))
+
+
+def get_artifact_volume(artifact: Artifact, sample_volume_udf: str) -> float:
+    """Return volume value from artifact."""
+    return float(get_udf(entity=artifact, udf=sample_volume_udf))
+
+
+def get_total_volume(artifact: Artifact, total_volume_udf: str) -> float:
+    """Return total volume value from artifact."""
+    return float(get_udf(entity=artifact, udf=total_volume_udf))
+
+
+def get_process_total_volume(process: Process, total_volume_udf: str) -> Optional[float]:
+    """Return total volume value from process."""
+    return process.udf.get(total_volume_udf)