change constants for minimum lane volume

[idalindegaard]

Added

Changed

• Constants for minimum volume per lane for flowcells 1.5, 10 and 25B with +1ul. see lims issue 522

Fixed

Steps to consider while deploying

• Configuration changes:
• Documentation updates:
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• Code approved by
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• "Merge and deploy" approved by

This version is a:

• MAJOR - when you make incompatible API changes
• MINOR - when you add functionality in a backwards compatible manner
• PATCH - when you make backwards compatible bug fixes or documentation/instructions

[Karl-Svard]

👍

Just one question, won't this affect the ratio of denaturation agents and such?

[idalindegaard]

👍

Thanks for the review, I was also planning on doing a quick test and add screenshots.

Just one question, won't this affect the ratio of denaturation agents and such?

Good question! This will not affect the ratio of reagents since this is not the final volume taken to the denaturation process and further to the library tube strip. The volume affected is just the bulk pool volume from which we will aliquot to a plate (where the denaturation process will take place)

[idalindegaard]

Testing the volume calculation with the 1.5B, 10B and 25B flowcell modes, first with one lane and then with eight lanes with a minimal sample volume - the screenshots below show a desired result. With eight lanes the Adjusted bulk pool volume should be 8*(changed volume constant).

The change of the volume constants does not change the settings/view in the Make Pool and Denature (NovaSeq X) step - which is correct, the volume change is only supposed to change the Adjusted bulk pool volume from which we take the volumes that are supposed to be denatured later. See image below.