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Update README-snv_plotter_pcaumaptsne.md
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smartinezzz authored May 15, 2024
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Expand Up @@ -11,7 +11,7 @@ in the dataset and utilize existing packages, such as Seurat, Slingshot, and scT
## Input
The script accepts three inputs:
- STAR solo output directory (-m) that contains features.tsv.gz, barcodes.tsv.gz, and matrix.mtx.gz OR a Seurat object (-r) that was saved as a .RDS
- SCReadCounts output file (-t) saved as a .tsv
- SCReadCounts output file (-t) that is tab-delimited and either a .tsv or .txt

See sample files for reference.

Expand All @@ -37,7 +37,7 @@ The produced figures include:
## Examples
ml R

Rscript 240508_multivar_plotter_tsne_blRed.r -t SNV_file.tsv -m SAMNXX_wasp_Solo.out/Gene/filtered/
Rscript 240508_multivar_plotter_tsne_blRed.r -t SNV_file.txt -m SAMNXX_wasp_Solo.out/Gene/filtered/

Rscript 240508_multivar_plotter_tsne_blRed.r -t SNV_file.tsv -m SAMNXX_wasp_Solo.out/Gene/filtered/ --th-vars=1 --th-reads=10 --tissue-type=Liver -c -d -e
Rscript 240508_multivar_plotter_tsne_blRed.r -t SNV_file.txt -m SAMNXX_wasp_Solo.out/Gene/filtered/ --th-vars=1 --th-reads=10 --tissue-type=Liver -c -d -e

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