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LupienLab/RepeatEnrichment
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Input to the pipeline: 1) Peaks folder consisting of all ATAC peaks bed file. 2) A consensus of all peaks bed file combined i.e. cat, merged and sorted. 3) Metadata file consisting of 4 columns rowsname "filename" "SampleName" "Group". Install 1) git clone https://github.com/LupienLab/RepeatEnrichment.git 2) GO to RepeatEnrichment/config_slurm/ and pull singularity image 3) run - singularity pull --arch amd64 library://nandankita/default/lupien-lab:ml_repeat_pipeline_v1.0 To run the pipeline: 1) Snakemake is installed/loaded means run this command: module load snakemake/5.20.1 2) Make sure you have all your input files. 3) Edit config.yaml to pass all correct variables. 4) from RepeatEnrichment directory run the command: sbatch config_slurm/run-pipeline.sh Singlarity is installed here on cluster: h4h: /cluster/tools/software/centos7/singularity/3.5.2/bin/singularity mordor: /mnt/work1/software/centos7/singularity/3.5.2/bin/singularity
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Repeat enrichment analysis
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