Update 05-lipid-and-fatty-acid-extraction-protocol-from-biological-sa…

…mples.md

updated reference & layout

docs/_Experimental-Procedure-Standards-SOPs/05-lipid-and-fatty-acid-extraction-protocol-from-biological-samples.md

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 Note: Method of measurement of cellular/microbial mass should be established with biologist/ microbiologist depending on experimental design. The method can be biomass weight or OD measurements which can be further used for normalization of lipid levels. Similar decision should be made for quenching procedures
 
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-Susan S. Bird, Vasant R. Marur, Matthew J. Sniatynski, Heather K. Greenberg, and Bruce S. Kristal., Serum Lipidomics Profiling using LC-MS and High Energy Collisional Dissociation Fragmentation: Focus on Triglyceride Detection and Characterization. Anal Chem. 2011 September 1; 83(17): 6648–6657. doi:10.1021/ac201195d. 
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 Specific instructions for homogenization of frozen tissues (eg; liver) prior to lipid extraction 
 1)	Starting with frozen tissue*, grind the tissue to a powder while frozen using a hand-held ceramic mortar and pestle. The mortar and pestle (any other apparatus eg; tweezers)  should be liquid nitrogen cooled before and should be kept cold throughout the process. A CryoMill can also be used for the homogenization of the tissue. 
 
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 *It is difficult to process tissue samples at weights below 10 mg, so aim for at least 10 mg of tissue to start with 
 
 
+Reference:
+{% cite <Bird_2011> %}
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