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ToryDeng committed Dec 22, 2023
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3 changes: 2 additions & 1 deletion README.md
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# LEGEND: An integrative algorithm for identifying co-expressed and cofunctional genes in multimodal transcriptomic sequencing data

We present a novel method called mu**L**timodal co-**E**xpressed **GE**nes fin**D**er (LEGEND) that performs integrated gene clustering on scRNA-seq and SRT data to identify co-expressed genes at both the cell type and tissue domain levels. LEGEND performs a hierarchical gene clustering with the aim of maximizing intra-cluster redundancy and inter-cluster complementarity.
![image](docs/assets/img/workflow.jpg)

![image](docs/assets/img/workflow.png)


## Dependencies
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title: LEGEND
logo: img/logo.png
logo: assets/img/logo.png
description: An integrative algorithm for identifying co-expressed and cofunctional genes in multi-omics.
show_downloads: false
google_analytics:
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4 changes: 2 additions & 2 deletions docs/index.md
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Exploring co-expressed genes is essential for revealing biologically co-functional genes. However, existing methods for this purpose rely solely on sc/snRNA-seq or spatially-resolved transcriptomic (SRT) data, leading to weaker co-functionality among identified genes. We present `LEGEND` (mu**L**timodal co-**E**xpressed **GE**nes fin**D**er), a novel method that performs integrated gene clustering on sc/snRNA-seq and SRT data for identifying genes co-expressed at both the cell type and tissue domain levels.


<img src="img/workflow.png" width="100%">
<img src="assets/img/workflow.png" width="100%">


The above figure illustrates the workflow of `LEGEND`. Under the framework of information theory, `LEGEND` estimates gene relevance, redundancy and complementarity in both SRT and sc/snRNA-seq datasets in a pseudo-semi-supervised manner. This information is used to construct a gene-gene redundancy graph, on which hierarchical gene clustering is performed using relative redundancy index (RRI) between neighboring gene nodes. The resulting clusters contain genes that are co-expressed at both tissue domain and cell type levels, suggesting a higher likelihood of biological co-functionality.
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sq.pl.spatial_scatter(adata_st, layer='STAGATE_ReX', color=["Caly", "Zcchc18"], figsize=(5, 5))
```

<img src="img/co-expression.png" width="100%">
<img src="assets/img/co-expression.png" width="100%">

Areas with high expression of one gene correlate with high expression of the other, and similarly for low expression areas. This illustrates the effectiveness of `LEGEND` in identifying biologically relevant gene clusters.

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