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@@ -111,7 +111,7 @@ picard CreateSequenceDictionary R={ref} O={ref.replace('.fa','.dict') | |
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1) Run it start-to-finish and submit a SBATCH job: | ||
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``` | ||
#!/bin/bash | ||
#SBATCH --job-name=searcHPV | ||
#SBATCH --mail-user=[email protected] | ||
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@@ -128,19 +128,19 @@ picard CreateSequenceDictionary R={ref} O={ref.replace('.fa','.dict') | |
source ~/anaconda3/etc/profile.d/conda.sh; | ||
conda activate searcHPV; | ||
searcHPV -fastq1 Sample_81279.R1.fastq.gz -fastq2 Sample_81279.R2.fastq.gz -humRef hs37d5.fa -virRef HPV.fa -output /home/scratch/HPV_fusion/Sample_81279 -gz -index; | ||
``` | ||
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2) Run it step-by-step: | ||
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``` | ||
searchHPV -alignment -fastq1 Sample_81279.R1.fastq.gz -fastq2 Sample_81279.R2.fastq.gz -humRef hs37d5.fa -virRef HPV.fa -output /home/scratch/HPV_fusion/Sample_81279 -gz -index | ||
searchHPV -genomeFusion -fastq1 Sample_81279.R1.fastq.gz -fastq2 Sample_81279.R2.fastq.gz -humRef hs37d5.fa -virRef HPV.fa -output /home/scratch/HPV_fusion/Sample_81279 -gz | ||
searchHPV -assemble -fastq1 Sample_81279.R1.fastq.gz -fastq2 Sample_81279.R2.fastq.gz -humRef hs37d5.fa -virRef HPV.fa -output /home/scratch/HPV_fusion/Sample_81279 -gz | ||
searchHPV -hpvFusion -fastq1 Sample_81279.R1.fastq.gz -fastq2 Sample_81279.R2.fastq.gz -humRef hs37d5.fa -virRef HPV.fa -output /home/scratch/HPV_fusion/Sample_81279 -gz | ||
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``` | ||
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Note: if run it step-by-step, please make sure the output directories for all steps are the same. | ||
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