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update usage
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WenjinGudaisy committed Feb 1, 2022
1 parent 681523f commit 1aa2b7e
Showing 1 changed file with 4 additions and 4 deletions.
8 changes: 4 additions & 4 deletions docs/index.md
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Expand Up @@ -111,7 +111,7 @@ picard CreateSequenceDictionary R={ref} O={ref.replace('.fa','.dict')

1) Run it start-to-finish and submit a SBATCH job:

```
#!/bin/bash
#SBATCH --job-name=searcHPV
#SBATCH --mail-user=[email protected]
Expand All @@ -128,19 +128,19 @@ picard CreateSequenceDictionary R={ref} O={ref.replace('.fa','.dict')
source ~/anaconda3/etc/profile.d/conda.sh;
conda activate searcHPV;
searcHPV -fastq1 Sample_81279.R1.fastq.gz -fastq2 Sample_81279.R2.fastq.gz -humRef hs37d5.fa -virRef HPV.fa -output /home/scratch/HPV_fusion/Sample_81279 -gz -index;
```



2) Run it step-by-step:


```
searchHPV -alignment -fastq1 Sample_81279.R1.fastq.gz -fastq2 Sample_81279.R2.fastq.gz -humRef hs37d5.fa -virRef HPV.fa -output /home/scratch/HPV_fusion/Sample_81279 -gz -index
searchHPV -genomeFusion -fastq1 Sample_81279.R1.fastq.gz -fastq2 Sample_81279.R2.fastq.gz -humRef hs37d5.fa -virRef HPV.fa -output /home/scratch/HPV_fusion/Sample_81279 -gz
searchHPV -assemble -fastq1 Sample_81279.R1.fastq.gz -fastq2 Sample_81279.R2.fastq.gz -humRef hs37d5.fa -virRef HPV.fa -output /home/scratch/HPV_fusion/Sample_81279 -gz
searchHPV -hpvFusion -fastq1 Sample_81279.R1.fastq.gz -fastq2 Sample_81279.R2.fastq.gz -humRef hs37d5.fa -virRef HPV.fa -output /home/scratch/HPV_fusion/Sample_81279 -gz

```

Note: if run it step-by-step, please make sure the output directories for all steps are the same.

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