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ksilnoaa committed Aug 29, 2023
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Expand Up @@ -199,6 +199,12 @@ Now edit, replace, or store the required input files as described here:

### Run Snakemake

Tourmaline is now run within the **snakemake** conda environment, not the qiime2-2023.5 environment.

```bash
conda activate snakemake
```

Shown here is the DADA2 paired-end workflow. See the Wiki's [Run](https://github.com/aomlomics/tourmaline/wiki/4-Run) page for complete instructions on all steps, denoising methods, and filtering modes.

Note that any of the commands below can be run with various options, including `--printshellcmds` to see the shell commands being executed and `--dryrun` to display which rules would be run but not execute them. To generate a graph of the rules that will be run from any Snakemake command, see the section "Directed acyclic graph (DAG)" on the [Run](https://github.com/aomlomics/tourmaline/wiki/4-Run) page. **Always include the --use-conda option.**
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