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Merge pull request #6 from thecaffiend/patch-1
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Update beginners-guide.md
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rpetit3 authored Jun 28, 2024
2 parents 9b7be66 + 6d0c41e commit 01fd4d9
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Expand Up @@ -68,7 +68,7 @@ Bactopia on. We will look at the following inputs:

### Illumina and/or Nanopore Reads

Let's start with the most common inputs, plain on FASTQ files for a _single sample_. Bactopia
Let's start with the most common inputs, plain old FASTQ files for a _single sample_. Bactopia
accepts both Illumina (pair-end or single-end) and Nanopore reads, and can even process them
together for a hybrid assembly.

Expand Down Expand Up @@ -218,7 +218,7 @@ file will be processed by Bactopia just like your normal local FASTQs.
!!! question "Why only Experiment accessions?"
In the grand scheme of accession hierarchies, Experiment accessions are really the only
unique ones. For example, a multiple Run accessions can be associated with a single
Experiment accession. Or, multiple Exeriment accessions can be associated with a single
Experiment accession. Or, multiple Experiment accessions can be associated with a single
BioSample accession. So, by using Experiment accessions, you can be confident you are
getting only the sequences associated with that "_unique_" Experiment.

Expand Down Expand Up @@ -247,7 +247,7 @@ to go fetch the associated assembly and process it just like a local assembly.
```

!!! question "Do I need to provide the assembly version? (e.g. GCF_000000000.1)"
Overtime I've found the assembly version to be unstable. For example, sometimes
Over time I've found the assembly version to be unstable. For example, sometimes
an assembly might be corrected, and the previous version is not made available
any longer. So, to avoid any issues, Bactopia will always use the latest version
of a given NCBI Assembly accession.
Expand Down Expand Up @@ -623,7 +623,7 @@ With this, Nextflow will use Docker to run all the processes in Bactopia (even t
Bactopia is installed with Conda!).

!!! tip "Always prefer containers over Conda"
While I will be the first to admit that I love Conda, it is not perfect. Overtime tools
While I will be the first to admit that I love Conda, it is not perfect. Over time tools
can become broken or incompatible due to dependencies. Containers are a great way
to avoid these issues. If you are using Bactopia, and have Docker or Singularity
available I would recommend using them over Conda.
Expand Down Expand Up @@ -742,4 +742,4 @@ successfully, the appropriate results are pulled out and placed in the sample's
The `work` directory can grow very large very quickly! Please keep this in mind when using
Bactopia (_and other Nextflow pipelines_). To help prevent the build up of the `work`
directory you can use `--cleanup_workdir` to automatically delete the `work` directory after
a successful run.
a successful run.

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