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Add Cell Count to SubCluster Morpheus Heatmap #33

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gwaybio opened this issue Nov 13, 2019 · 0 comments
Open

Add Cell Count to SubCluster Morpheus Heatmap #33

gwaybio opened this issue Nov 13, 2019 · 0 comments

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@gwaybio
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gwaybio commented Nov 13, 2019

in #25 I performed a subcluster analysis to identify differential features across mutant and wild-type clones in each cluster independently.

There is one large big red cluster in the heatmap. We noticed that random example images from this block had high confluence (see below).

To more systematically address this question, I will add cell count to morpheus heatmaps.

Cells in Big Red Cluster

WT Clones in Well C02

image

Mut Clones in Well B06

image

WT Parental Clones in D11

image

Cells not in big red cluster

Mut Clones in D09

image

WT Clones in E05

image

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