Skip to content

Commit

Permalink
Merge branch 'develop' into lk-PD2432-gex-barcodes-to-fragment-file
Browse files Browse the repository at this point in the history
  • Loading branch information
ekiernan authored Nov 30, 2023
2 parents a9f5040 + 41bb4b3 commit a1403e5
Show file tree
Hide file tree
Showing 13 changed files with 23 additions and 18 deletions.
Original file line number Diff line number Diff line change
@@ -1,7 +1,8 @@
# 3.1.14
2023-11-21 (Date of Last Commit)
2023-11-29 (Date of Last Commit)

* Fixed bug in ReblockGVCFs; this does not affect this pipeline.
* Reverted the VerifyBamID docker image back to the 3.1.10 ExomeGermlineSingleSample pipeline version

# 3.1.13
2023-10-10 (Date of Last Commit)
Expand Down
Original file line number Diff line number Diff line change
Expand Up @@ -2,6 +2,7 @@
2023-11-21 (Date of Last Commit)

* Fixed bug in ReblockGVCFs; this does not affect this pipeline.
* Reverted the VerifyBamID docker image back to the 1.0.9 UltimaGenomicsWholeGenomeGermline pipeline version

# 1.0.10
2023-09-18 (Date of Last Commit)
Expand Down
Original file line number Diff line number Diff line change
@@ -1,7 +1,8 @@
# 3.1.15
2023-11-21 (Date of Last Commit)
2023-11-29 (Date of Last Commit)

* Fixed bug in ReblockGVCFs; this does not affect this pipeline.
* Reverted the VerifyBamID docker image back to the 3.1.11 WholeGenomeGermlineSingleSample pipeline version

# 3.1.14
2023-10-10 (Date of Last Commit)
Expand Down
Original file line number Diff line number Diff line change
@@ -1,7 +1,8 @@
# 2.1.13
2023-11-21 (Date of Last Commit)
2023-11-29 (Date of Last Commit)

* Fixed bug in ReblockGVCFs; this does not affect this pipeline.
* Reverted the VerifyBamID docker image back to the 2.1.10 VariantCalling pipeline version

# 2.1.12
2023-09-18 (Date of Last Commit)
Expand Down
Original file line number Diff line number Diff line change
@@ -1,7 +1,8 @@
# 1.0.11
2023-11-21 (Date of Last Commit)
2023-11-29 (Date of Last Commit)

* Fixed bug in ReblockGVCFs; this does not affect this pipeline.
* Reverted the VerifyBamID docker image back to the 1.0.8 UltimaGenomicsWholeGenomeCramOnly pipeline version

# 1.0.10
2023-09-18 (Date of Last Commit)
Expand Down
Original file line number Diff line number Diff line change
@@ -1,7 +1,8 @@
# 1.0.12
2023-11-21 (Date of Last Commit)
2023-11-29 (Date of Last Commit)

* Fixed bug in ReblockGVCFs; this does not affect this pipeline.
* Reverted the VerifyBamID docker image back to the 1.0.9 BroadInternalUltimaGenomics pipeline version

# 1.0.11
2023-09-18 (Date of Last Commit)
Expand Down
Original file line number Diff line number Diff line change
@@ -1,7 +1,8 @@
# 3.1.14
2023-11-21 (Date of Last Commit)
2023-11-29 (Date of Last Commit)

* Fixed bug in ReblockGVCFs; this does not affect this pipeline.
* Reverted the VerifyBamID docker image back to the 3.1.10 ExomeReprocessing pipeline version

# 3.1.13
2023-10-10 (Date of Last Commit)
Expand Down
Original file line number Diff line number Diff line change
@@ -1,7 +1,8 @@
# 3.1.16
2023-11-21 (Date of Last Commit)
2023-11-29 (Date of Last Commit)

* Fixed bug in ReblockGVCFs; this does not affect this pipeline.
* Reverted the VerifyBamID docker image back to the 3.1.12 ExternalExomeReprocessing pipeline version

# 3.1.15
2023-10-10 (Date of Last Commit)
Expand Down
Original file line number Diff line number Diff line change
@@ -1,7 +1,8 @@
# 2.1.16
2023-11-21 (Date of Last Commit)
2023-11-29 (Date of Last Commit)

* Fixed bug in ReblockGVCFs; this does not affect this pipeline.
* Reverted the VerifyBamID docker image back to the 2.1.12 ExternalWholeGenomeReprocessing pipeline version

# 2.1.15
2023-10-10 (Date of Last Commit)
Expand Down
Original file line number Diff line number Diff line change
@@ -1,7 +1,8 @@
# 3.1.15
2023-11-21 (Date of Last Commit)
2023-11-29 (Date of Last Commit)

* Fixed bug in ReblockGVCFs; this does not affect this pipeline.
* Reverted the VerifyBamID docker image back to the 3.1.11 WholeGenomeReprocessing pipeline version

# 3.1.14
2023-10-10 (Date of Last Commit)
Expand Down
6 changes: 1 addition & 5 deletions tasks/broad/BamProcessing.wdl
Original file line number Diff line number Diff line change
Expand Up @@ -417,9 +417,7 @@ task CheckContamination {
Float contamination_underestimation_factor
Boolean disable_sanity_check = false

# this docker image was built using the commit hash c1cba76e979904eb69c31520a0d7f5be63c72253 (v1.0.6)from the VerifyBamID repo
# https://github.com/Griffan/VerifyBamID/commit/c1cba76e979904eb69c31520a0d7f5be63c72253
String docker = "us.gcr.io/broad-gotc-prod/verify-bam-id:1.0.1-c1cba76e979904eb69c31520a0d7f5be63c72253-1692099985"
String docker = "us.gcr.io/broad-gotc-prod/verify-bam-id:1.0.1-c1cba76e979904eb69c31520a0d7f5be63c72253-1639071840"
}

Int disk_size = ceil(size(input_bam, "GiB") + size(ref_fasta, "GiB")) + 30
Expand Down Expand Up @@ -468,8 +466,6 @@ task CheckContamination {
preemptible: preemptible_tries
memory: "7.5 GiB"
disks: "local-disk " + disk_size + " HDD"
# this docker image was built using the commit hash c1cba76e979904eb69c31520a0d7f5be63c72253 from the VerifyBamID repo
# https://github.com/Griffan/VerifyBamID/commit/c1cba76e979904eb69c31520a0d7f5be63c72253
docker: docker
cpu: 2
}
Expand Down
5 changes: 2 additions & 3 deletions tasks/broad/UltimaGenomicsWholeGenomeGermlineTasks.wdl
Original file line number Diff line number Diff line change
Expand Up @@ -390,9 +390,8 @@ task CheckContamination {
References references
String output_prefix
# this docker image was built using the commit hash f6cb51761861e57c43879aa262df5cf8e670cf7c (v2.0.1) from the VerifyBamID repo
# https://github.com/Griffan/VerifyBamID/commit/f6cb51761861e57c43879aa262df5cf8e670cf7c
String docker = "us.gcr.io/broad-gotc-prod/verify-bam-id:1.0.1-f6cb51761861e57c43879aa262df5cf8e670cf7c-1692216226"
String docker = "us.gcr.io/broad-gotc-prod/verify-bam-id:f6cb51761861e57c43879aa262df5cf8e670cf7c-1606775309"
Int disk_size_gb = ceil(if ceil((size(input_bam, "GB")) +
(size(references.ref_fasta, "GB") +
size(references.ref_fasta_index, "GB") +
Expand Down
2 changes: 1 addition & 1 deletion website/docs/Pipelines/ATAC/README.md
Original file line number Diff line number Diff line change
Expand Up @@ -71,7 +71,7 @@ To see specific tool parameters, select the task WDL link in the table; then vie

| Task name and WDL link | Tool | Software | Description |
| --- | --- | --- | ------------------------------------ |
| [FastqProcessing as SplitFastq](https://github.com/broadinstitute/warp/blob/master/tasks/skylab/FastqProcessing.wdl) | fastqprocess | custom | Dynamically selects the correct barcode orientation, corrects cell barcodes and splits FASTQs into 30 GB FASTQs that are grouped by cell barcode with each read having the corrected (CB) and raw barcode (CR) in the read name. |
| [FastqProcessing as SplitFastq](https://github.com/broadinstitute/warp/blob/master/tasks/skylab/FastqProcessing.wdl) | fastqprocess | custom | Dynamically selects the correct barcode orientation, corrects cell barcodes and splits FASTQs into smaller FASTQs. The number of files output depends on either the bam_size parameter, which determines the size of the output FASTQs produced, or the num_output_files parameter, which determines the number of FASTQS that should be output. The smaller FASTQs are grouped by cell barcode with each read having the corrected (CB) and raw barcode (CR) in the read name. |
| [TrimAdapters](https://github.com/broadinstitute/warp/blob/master/pipelines/skylab/multiome/atac.wdl) | Cutadapt v4.4 | cutadapt | Trims adaptor sequences. |
| [BWAPairedEndAlignment](https://github.com/broadinstitute/warp/blob/master/pipelines/skylab/multiome/atac.wdl) | bwa-mem2 | mem | Aligns reads from each set of partitioned FASTQ files to the genome and outputs a BAM with ATAC barcodes in the CB:Z tag. |
| [Merge.MergeSortBamFiles as MergeBam](https://github.com/broadinstitute/warp/blob/master/tasks/skylab/MergeSortBam.wdl) | MergeSamFiles | Picard | Merges each BAM into a final aligned BAM with corrected cell barcodes in the CB tag. |
Expand Down

0 comments on commit a1403e5

Please sign in to comment.