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Lk atac percent target #1419

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6 changes: 3 additions & 3 deletions pipeline_versions.txt
Original file line number Diff line number Diff line change
Expand Up @@ -30,11 +30,11 @@ ExomeReprocessing 3.3.3 2024-11-04
BuildIndices 3.0.0 2023-12-06
scATAC 1.3.2 2023-08-03
snm3C 4.0.4 2024-08-06
Multiome 5.9.0 2024-10-21
PairedTag 1.8.1 2024-11-04
Multiome 5.9.1 2024-11-12
PairedTag 1.8.2 2024-11-12
MultiSampleSmartSeq2 2.2.22 2024-09-11
MultiSampleSmartSeq2SingleNucleus 2.0.3 2024-11-04
Optimus 7.8.1 2024-11-04
atac 2.5.0 2024-10-23
atac 2.5.1 2024-11-12
SmartSeq2SingleSample 5.1.21 2024-09-11
SlideSeq 3.4.4 2024-11-04
6 changes: 6 additions & 0 deletions pipelines/skylab/atac/atac.changelog.md
Original file line number Diff line number Diff line change
@@ -1,7 +1,13 @@
# 2.5.1
2024-11-12 (Date of Last Commit)

* Renamed the ATAC workflow library metric percent_target to atac_percent_target for compatibility with downstream tools

# 2.5.0
2024-10-23 (Date of Last Commit)

* Updated the tabix flag in CreateFragmentFile task to use CSI instead of TBI indexing, which supports chromosomes larger than 512 Mbp
* Renamed the ATAC workflow library metric percent_target to atac_percent_target for compatibility with downstream tools

# 2.4.0
2024-10-23 (Date of Last Commit)
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4 changes: 2 additions & 2 deletions pipelines/skylab/atac/atac.wdl
Original file line number Diff line number Diff line change
Expand Up @@ -49,7 +49,7 @@ workflow ATAC {
String adapter_seq_read3 = "TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG"
}

String pipeline_version = "2.5.0"
String pipeline_version = "2.5.1"

# Determine docker prefix based on cloud provider
String gcr_docker_prefix = "us.gcr.io/broad-gotc-prod/"
Expand Down Expand Up @@ -575,7 +575,7 @@ task CreateFragmentFile {
print("Print number of cells", number_of_cells)
atac_percent_target = number_of_cells / expected_cells*100
print("Setting percent target in nested dictionary")
data['Cells']['percent_target'] = atac_percent_target
data['Cells']['atac_percent_target'] = atac_percent_target


# Flatten the dictionary
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8 changes: 8 additions & 0 deletions pipelines/skylab/multiome/Multiome.changelog.md
Original file line number Diff line number Diff line change
@@ -1,7 +1,15 @@
# 5.9.1
2024-11-12 (Date of Last Commit)

* Renamed the ATAC workflow library metric percent_target to atac_percent_target for compatibility with downstream tools

# 5.9.0
2024-10-21 (Date of Last Commit)

* Updated the tabix flag in JoinMultiomeBarcodes task in H5adUtils.wdl to use CSI instead of TBI indexing, which supports chromosomes larger than 512 Mbp; this task changes the format for the ATAC fragment file index
* Renamed the fragment file index from atac_fragment_tsv_tbi to atac_fragment_tsv_index


# 5.8.0
2024-10-23 (Date of Last Commit)

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2 changes: 1 addition & 1 deletion pipelines/skylab/multiome/Multiome.wdl
Original file line number Diff line number Diff line change
Expand Up @@ -9,7 +9,7 @@ import "../../../tasks/broad/Utilities.wdl" as utils

workflow Multiome {

String pipeline_version = "5.9.0"
String pipeline_version = "5.9.1"

input {
String cloud_provider
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5 changes: 5 additions & 0 deletions pipelines/skylab/paired_tag/PairedTag.changelog.md
Original file line number Diff line number Diff line change
@@ -1,3 +1,8 @@
# 1.8.2
2024-11-12 (Date of Last Commit)

* Renamed the ATAC workflow library metric percent_target to atac_percent_target for compatibility with downstream tools

# 1.8.1
2024-11-04 (Date of Last Commit)

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2 changes: 1 addition & 1 deletion pipelines/skylab/paired_tag/PairedTag.wdl
Original file line number Diff line number Diff line change
Expand Up @@ -8,7 +8,7 @@ import "../../../tasks/broad/Utilities.wdl" as utils

workflow PairedTag {

String pipeline_version = "1.8.1"
String pipeline_version = "1.8.2"


input {
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2 changes: 1 addition & 1 deletion website/docs/Pipelines/ATAC/README.md
Original file line number Diff line number Diff line change
Expand Up @@ -8,7 +8,7 @@ slug: /Pipelines/ATAC/README

| Pipeline Version | Date Updated | Documentation Author | Questions or Feedback |
| :----: | :---: | :----: | :--------------: |
| [2.4.0](https://github.com/broadinstitute/warp/releases) | October, 2024 | WARP Pipelines | Please [file an issue in WARP](https://github.com/broadinstitute/warp/issues). |
| [2.5.1](https://github.com/broadinstitute/warp/releases) | November, 2024 | WARP Pipelines | Please [file an issue in WARP](https://github.com/broadinstitute/warp/issues). |


## Introduction to the ATAC workflow
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2 changes: 1 addition & 1 deletion website/docs/Pipelines/ATAC/library-metrics.md
Original file line number Diff line number Diff line change
Expand Up @@ -31,6 +31,6 @@ The [ATAC pipeline](README.md) uses [SnapATAC2](https://github.com/kaizhang/Snap
| Number_of_peaks | The total number of peaks, or regions of accessible chromatin, identified in the dataset, representing potential regulatory elements. |
| fraction_of_genome_in_peaks | The fraction of the genome that is covered by identified peaks, indicating the extent of chromatin accessibility across the genome. |
| fraction_of_high-quality_fragments_overlapping_peaks | The fraction of high-quality fragments that overlap with identified peaks, providing an indication of the efficiency of the assay in capturing accessible regions. |
| percent_target | Percent of cells recovered; value is calculated as estimated_cells/expected_cells. |
| atac_percent_target | Percent of cells recovered; value is calculated as estimated_cells/expected_cells. |


6 changes: 3 additions & 3 deletions website/docs/Pipelines/Multiome_Pipeline/README.md
Original file line number Diff line number Diff line change
Expand Up @@ -7,7 +7,7 @@ slug: /Pipelines/Multiome_Pipeline/README

| Pipeline Version | Date Updated | Documentation Author | Questions or Feedback |
| :----: | :---: | :----: | :--------------: |
| [Multiome v5.8.0](https://github.com/broadinstitute/warp/releases) | October, 2024 | WARP Pipelines | Please [file an issue in WARP](https://github.com/broadinstitute/warp/issues). |
| [Multiome v5.9.1](https://github.com/broadinstitute/warp/releases) | November, 2024 | WARP Pipelines | Please [file an issue in WARP](https://github.com/broadinstitute/warp/issues). |

![Multiome_diagram](./multiome_diagram.png)

Expand Down Expand Up @@ -108,9 +108,9 @@ The Multiome workflow calls two WARP subworkflows, one external subworkflow (opt
| multiome_pipeline_version_out | N.A. | String describing the version of the Multiome pipeline used. |
| bam_aligned_output_atac | `<input_id>_atac.bam` | BAM file containing aligned reads from ATAC workflow. |
| fragment_file_atac | `<input_id>_atac.fragments.sorted.tsv.gz` | Sorted and bgzipped TSV file containing fragment start and stop coordinates per barcode. The columns are "Chromosome", "Start", "Stop", "ATAC Barcode", "Number of reads", and "GEX Barcode". |
| fragment_file_index | `<input_id>_atac.fragments.sorted.tsv.gz.tbi` | tabix index file for the fragment file. |
| fragment_file_index | `<input_id>_atac.fragments.sorted.tsv.gz.csi` | Tabix CSI index file for the fragment file. |
| snap_metrics_atac | `<input_id>_atac.metrics.h5ad` | h5ad (Anndata) file containing per-barcode metrics from SnapATAC2. Also contains the equivalent gene expression barcode for each ATAC barcode in the `gex_barcodes` column of the `h5ad.obs` property. See the [ATAC Count Matrix Overview](../ATAC/count-matrix-overview.md) for more details. |
| atac_library_metrics | `<input_id>_atac_<nhash_id>.metrics.csv` | CSV with library-level metrics produced by SnapATAC2. See the ATAC [Library Level Metrics Overview](../ATAC/library-metrics.md) for more details. |
| atac_library_metrics | `<input_id>_atac_<nhash_id>_library_metrics.csv` | CSV with library-level metrics produced by SnapATAC2. See the ATAC [Library Level Metrics Overview](../ATAC/library-metrics.md) for more details. |
| genomic_reference_version_gex | `<reference_version>.txt` | File containing the Genome build, source and GTF annotation version. |
| bam_gex | `<input_id>_gex.bam` | BAM file containing aligned reads from Optimus workflow. |
| matrix_gex | `<input_id>_gex_sparse_counts.npz` | NPZ file containing raw gene by cell counts. |
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2 changes: 1 addition & 1 deletion website/docs/Pipelines/PairedTag_Pipeline/README.md
Original file line number Diff line number Diff line change
Expand Up @@ -7,7 +7,7 @@ slug: /Pipelines/PairedTag_Pipeline/README

| Pipeline Version | Date Updated | Documentation Author | Questions or Feedback |
|:---:| :---: | :---: | :---: |
| [PairedTag_v1.8.0](https://github.com/broadinstitute/warp/releases) | October, 2024 | WARP Pipelines | Please [file an issue in WARP](https://github.com/broadinstitute/warp/issues). |
| [PairedTag_v1.8.2](https://github.com/broadinstitute/warp/releases) | November, 2024 | WARP Pipelines | Please [file an issue in WARP](https://github.com/broadinstitute/warp/issues). |


## Introduction to the Paired-Tag workflow
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