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genomewalker committed Sep 30, 2024
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4 changes: 2 additions & 2 deletions .github/workflows/filterBAM_ci.yml
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Expand Up @@ -11,8 +11,8 @@ jobs:
- uses: actions/checkout@v2
- uses: conda-incubator/setup-miniconda@v2
with:
python-version: 3.9
mamba-version: "*"
python-version: "3.9"
solver: "libmamba"
channels: conda-forge,bioconda,defaults
channel-priority: true
environment-file: environment.yml
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4 changes: 2 additions & 2 deletions README.md
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Expand Up @@ -241,7 +241,7 @@ filterBAM filter --bam c55d4e2df1.reassigned.bam --bam-filtered c55d4e2df1.dedup
**--min-read-ani**: Minimum average read ANI that a reference has
**--sort-by-name**: Sort filtered BAM file by read name so it can be used in [metaDMG](https://metadmg-dev.github.io/metaDMG-core/)
**--sort-by-name**: Sort filtered BAM file by read name so it can be used in [metaDMG](https://github.com/metaDMG-dev/metaDMG-cpp)
**--sort-memory**: Memory used for each thread when sorting the filtered BAM file
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### Applications and recommendations
One of the main applications of **bam-filter** is to reliably identify which potential organisms are present in a metagenomic ancient sample, and get relatively accurate taxonomic abundances, even when they are present in very low abundances. The resulting BAM file then can be used as input for [metaDMG](https://metadmg-dev.github.io/metaDMG-core/). We rely on several measures to discriminate between noise and a potential signal, analyzing the mapping results at two different levels:
One of the main applications of **bam-filter** is to reliably identify which potential organisms are present in a metagenomic ancient sample, and get relatively accurate taxonomic abundances, even when they are present in very low abundances. The resulting BAM file then can be used as input for [metaDMG](https://github.com/metaDMG-dev/metaDMG-cpp). We rely on several measures to discriminate between noise and a potential signal, analyzing the mapping results at two different levels:
- Is the observed breadth aligned with the expected one?
- Are the reads spread evenly across the reference or they are clumped in a few regions?
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