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iliapopov17 committed Sep 29, 2024
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Expand Up @@ -27,24 +27,4 @@ _Typical workflow of NGS data analysis_
┃ ┗ 📃05_03_Parkinsons_disease<br>
┗ ℹ️ README.md

_Handbook's structure_

## 05 16S Amplicon Analysis 🧫

In the [16S amplicon analysis folder](05_16S_amplicon_analysis) there is an introductory guide on conducting analysis using `DADA2` followed by two interesting examples of "real-life" analysis pipeline with the data from studies on Crohn's and Parkinson's diseases.

## 04 Phylogenetics 🌳

In the [Phylogenetics folder](04_Phylogenetics) there is a complete pipeline of simple research in phylogenetics, from working with NCBI (and other databases) to building trees, evaluating them, and getting some worthwhile results.

## 03 Whole Genome and Pangenome Analyses 🧬

In the [Whole (pan)genome analyses](03_Whole_(pan)genome_analyses) there is a pipeline of whole genome and pangenome analyses with `PanACoTA` pipeline which includes genomes filtering with `mash`, annotating with `prokka` & `prodigal`, pangenome building with `mmseqs`, core genomes alignment with `mafft` and finally building phylogenetic tree with `iq-tree`.

## 02 Genomic Variation Analysis 🔬

In the [Genomic Variation Analysis folder](02_Genomic_Variation_Analysis) there is a detailed guide how to conduct studies on Variant Calling using `fastqc`, `trimmomatic`, `bwa`, `samtools`, `abra2`, `bcftools`, `snpEff` & `SnpSift`.

## 01 Quality Control of raw data 💎

In the [Quality Control folder](01_Quality_Control) there is a detailed guide how to conduct quality control of raw data using `fastqc` and `trimmomatic`.
_Handbook's structure_

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