SRA Metadata toolkit

currently hosting at http://g86.dbcls.jp/sra as a beta version. (sometimes responce is very slow, sorry!)

Table of Contents

• 1 What's this
• 2 How to use
  • 2.1 SRA ID Converter
  • 2.2 SRA Metadata parser
  • 2.3 FastQC result
    • 2.3.1 readfile
• 3 Development
  • 3.1 About this project
  • 3.2 License

1 What's this

SRA Metadata toolkit is the project and the web service to use Metadata of the NGSeq data archived in Sequence Read Archive. It allows command-line access to get JSON format data parsed as specified with query. It also provides quality summary of each sequence data. Details and updates are here.

2 How to use

User's can get a part or whole set of metadata by specifying with query.

2.1 SRA ID Converter

convert SRA ID to belonged/related ID(s). JSON format output

/idconvert/<origin SRA ID>.to_<dest SRA ID type>

<origin SRA ID>

valid SRA ID, three letters prefix and six numbers

<dest SRA ID type>

submission, study, experiment, sample, run, pmid, all

example:

/idconvert/DRA000001.to_study
  => ["DRP000001"]
/idconvert/DRA000001.to_all
  => {"submission":["DRA000001"],"study":["DRP000001"],"experiment":["DRX000001"],"sample":["DRS000001"],"run":["DRR000001"],"pmid":["20398357"]}

2.2 SRA Metadata parser

gives JSON format metadata for specified SRA ID

/metadata/<origin SRA ID>.<method>

<origin SRA ID>

valid SRA ID, three letters perfix and six numbers

<method>

method to specify which part of metadata to be given. use ".methods" to get a list of valid methods.

example:

/metadata/DRA000001.submission_comment
  => ["Bacillus subtilis subsp. natto BEST195 draft sequence, the chromosome and plasmid pBEST195S"]
/metadata/DRA000001.all
  => {"submission_comment":"Bacillus subtilis subsp. natto BEST195 draft sequence, the chromosome and plasmid pBEST195S","center_name":"KEIO","lab_name":"Bioinformatics Lab.","submission_date":"2009-05-14 14:16:00 UTC","alias":"DRA000001"}
/metadata/DRA000001.methods
  => ["alias","submission_comment","center_name","lab_name","submission_date","all"]

2.3 FastQC result

gives JSON format result of FastQC if available

/fastqc/<origin sequence run file name>.<method>

<origin sequence run file name>

single sequence run file name, descriminate forward/reverse. list of run file those belong to run id can be got from "filename" api.

<method>

method to specify which part of metadata to be given. use ".methods" to get a list of valid methods.

2.3.1 readfile

A list of readfile

/readfile/<origin SRA Run ID>

example:

/readfile/DRR000001
  => ["DRR000001_1","DRR000001_2","DRR000001"]

3 Development

3.1 About this project

The main contributor of this project is me, Tazro Ohta, and that's all. Wow. This project is a part of large-scale nucleotide sequence archive team in DBCLS.

3.2 License

All code is shared under the Beer-ware License.