Microstacking

Microstacking is a project that allows you to control a camera and a stepper motor for focus stacking in a microscopy context. The application is built using Python and Tkinter for the GUI, and an Arduino for controlling the stepper motor.

Features

• Control camera settings such as shutter speed, ISO, white balance, and image format.
• Capture images and display them in a treeview.
• Control a stepper motor for precise focus stacking.
• Preview camera feed.
• Save captured images to a specified folder.

Requirements

• Python 3.x
• Tkinter
• ttkthemes
• PIL (Pillow)
• gphoto2
• pyserial
• Arduino with A4988 stepper motor driver

Installation

1. Clone the repository:

   git clone https://github.com/yourusername/microstacking.git
   cd microstacking

2. Install the required Python packages:

   pip install tk ttkthemes pillow gphoto2 pyserial

3. Upload the Arduino firmware to your Arduino board:

   cd firmware
   arduino --upload stepper_firmware.ino

Usage

1. Connect your camera and Arduino to your computer.
2. Run the Python application:

   python microstacking.py

Video Demonstration