You will need an internet connection to install this software.
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Install the National Instruments NIDAQmx driver. This software is provided by National Instruments.
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Install Python on a Windows computer. Make sure when downloading that you install the 64 bit 2.7 version. The version used during development is python-2.7.10.amd64.msi. This software has also been tested on Windows 64 bit Python 3.5.
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Download this respository.
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Double click the shadowlessTIRF.bat file. This should install all the dependencies and start the program.