-
Notifications
You must be signed in to change notification settings - Fork 6
Commit
This commit does not belong to any branch on this repository, and may belong to a fork outside of the repository.
- Loading branch information
Showing
4 changed files
with
85 additions
and
1 deletion.
There are no files selected for viewing
This file contains bidirectional Unicode text that may be interpreted or compiled differently than what appears below. To review, open the file in an editor that reveals hidden Unicode characters.
Learn more about bidirectional Unicode characters
Original file line number | Diff line number | Diff line change |
---|---|---|
@@ -0,0 +1,56 @@ | ||
# HiP-CT data | ||
|
||
HiP-CT stands for hierarchical phase-contrast tomography. | ||
For more information on the modality see the [HiP-CT website](https://mecheng.ucl.ac.uk/hip-ct/). | ||
Most of the time HiP-CT consists of a single 'low' (but still quite high!) resolution scan of a whole sample, and then one or more 'high' resolution scans of particular volumes of interest within the same sample. | ||
|
||
## Data information | ||
|
||
There are HiP-CT scans of two donors, S45 and I58. | ||
The data are part of the [Pilot human brains dataset](https://lincbrain.org/dandiset/000005) on lincbrain. | ||
|
||
Datasets have the name `sub-<donor>_sample-<sample name>_chunk<chunk number>_PC.ome.zarr`. | ||
The chunk number is set to `yyxx`, where yy is the resolution of the scan in micrometers, and xx is a scan number that is unique between datasets. | ||
For example, a scan number of `402` is the second 4um scan. | ||
The highest lowest resolution dataset (equivalently, highest chunk number) is the overview dataset that contains an image of the whole sample. | ||
There are then one or more datsets at higher resolution. | ||
|
||
## Registration | ||
|
||
Each high resolution dataset is registered to the overview dataset using [`hipct-reg`](https://github.com/hipCTProject/hipct-reg). | ||
Each transform maps the pixel coordinates of the high resolution zoom datasets to the overview full-sample dataset. | ||
The transform is of the form: | ||
|
||
$$ | ||
T(\mathbf{x}) = s \left ( \mathbf{R} \cdot\mathbf{x} \right ) + \mathbf{t} | ||
$$ | ||
|
||
Where | ||
|
||
- $s$ is a scaling factor | ||
- $\mathbf{R}$ is a rotation matrix that rotates about the z-axis. | ||
- $\mathbf{t}$ is a translation vector | ||
|
||
The rotation matrix takes the form | ||
|
||
$$ | ||
\mathbf{R} = \begin{pmatrix} | ||
\cos(-\theta) & \sin(-\theta) & 0 \\ | ||
-\sin(-\theta) & \cos(-\theta) & 0 \\ | ||
0 & 0 & 1 \\ | ||
\end{pmatrix} | ||
$$ | ||
|
||
The transform has 5 parameters (1 scaling, 1 rotation, 3 translation). | ||
|
||
### Registration accuracy | ||
|
||
The HiP-CT registration pipeline is designed to be accurate enough to approximately visually align two datasets. **It is not designed for pixel perfect registration.** We recommend anyone doing analysis relying on the registration between two datasets performs a more accurate registration themselves, using our registration as an initial starting point. | ||
|
||
### Registration data | ||
|
||
|
||
| Dataset | Overview dataset | $\mathbf{t}$ | $s$ | $\theta$ (degrees) | | ||
| -------- | -------- | -------- | -------- | -------- | | ||
| `sub-I58_sample-blockIC2_chunk-101_PC` | `sub-I58_sample-blockIC2_chunk-401_PC` | (3746.4467746089194, 3660.0610535229666, 1127.3616712249263) | 0.2016106611944697 | -6.496401244495998 | | ||
| `sub-I58_sample-blockIC2_chunk-201_PC` | `sub-I58_sample-blockIC2_chunk-401_PC` | (2119.695989239648, 2321.6572801771194, 971.0041825720597) | 0.5181803632402424 | 0.5004912711693281 | |
This file contains bidirectional Unicode text that may be interpreted or compiled differently than what appears below. To review, open the file in an editor that reveals hidden Unicode characters.
Learn more about bidirectional Unicode characters
Original file line number | Diff line number | Diff line change |
---|---|---|
@@ -0,0 +1,19 @@ | ||
window.MathJax = { | ||
tex: { | ||
inlineMath: [["\\(", "\\)"]], | ||
displayMath: [["\\[", "\\]"]], | ||
processEscapes: true, | ||
processEnvironments: true | ||
}, | ||
options: { | ||
ignoreHtmlClass: ".*|", | ||
processHtmlClass: "arithmatex" | ||
} | ||
}; | ||
|
||
document$.subscribe(() => { | ||
MathJax.startup.output.clearCache() | ||
MathJax.typesetClear() | ||
MathJax.texReset() | ||
MathJax.typesetPromise() | ||
}) |
Empty file.
This file contains bidirectional Unicode text that may be interpreted or compiled differently than what appears below. To review, open the file in an editor that reveals hidden Unicode characters.
Learn more about bidirectional Unicode characters